Hybrid Pectin-Liposome Formulation against Multi-Resistant Bacterial Strains

Ribeiro, Lígia Nunes de Morais; Paula, Eneida de; Rossi, Daise Aparecida; Monteiro, Guilherme Paz; Júnior, Edson Campos Valadares; Silva, Rogério Reis; Espíndola, Foued Salmen; Goulart, Luiz Ricardo; Fonseca, Belchiolina Beatriz

doi:10.3390/pharmaceutics12080769

Cited by 23 publications

(14 citation statements)

References 53 publications

(65 reference statements)

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“…However, blood samples collected in research with CE do not always allow this analysis because it is not easy and takes time. Therefore, alternatives samples have been used to assess these parameters, such as amniotic fluid and allantoic fluid (5,7,46). In this work, we compared the biochemical analyses of serum and allantoic fluid and observed that the values found for the two samples were not always similar.…”

Section: Discussionmentioning

confidence: 98%

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Physiological Changes in Chicken Embryos Inoculated With Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Sommerfeld

Mundim

Silva

et al. 2021

Preprint

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Background: Several works have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardise the evaluated parameters are needed to better understand and identify the viability of chicken embryos (CE) as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are peculiar to the model. To achieve this goal, in ovo assays were performed injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult chickens and other animals. Results: Embryo response to virus and drug challenges may not occur as expected for adult chickens and even different species. While macroscopic and microscopic damage was visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not result in the expected effects for CE. On the other hand, with dexamethasone (DX), changes in blood parameters and biomarkers were inherent to the model and depended on the stage of CE development.Conclusions: Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are recorded.

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Section: Discussionmentioning

confidence: 98%

“…Then, the embryo and yolk were weighed immediately after collecting blood and allantoic fluid on the day of collection. As the embryo weight is related to the initial egg weight, we performed an adjustment for an initial egg weight of 50 grams, according to Ribeiro et al (2020) (7) 2.4.…”

Section: Weight Of the Ce And Annexesmentioning

confidence: 99%

Physiological Changes in Chicken Embryos Inoculated With Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Sommerfeld

Mundim

Silva

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, blood samples collected in research with CE do not always allow this analysis because it is not easy and takes time. Therefore, alternatives samples have been used to assess these parameters, such as amniotic uid and allantoic uid 5,7,33 . In this work, we compared the biochemical analyses of serum and allantoic uid and observed that the values found for the two samples were not always similar.…”

Section: Discussionmentioning

confidence: 99%

“…Then, the embryo and yolk were weighed immediately after collecting blood and allantoic uid on the day of collection. As the embryo weight is related to the initial egg weight, we performed an adjustment for an initial egg weight of 50 grams, according to Ribeiro et al (2020) 7…”

Section: Weight Of the Ce And Annexesmentioning

confidence: 99%

“…Numerous works have been carried out using the Gallus gallus embryo as an experimental model for evaluating the toxic effects of drugs and infections. These studies usually evaluated blood, allantoic and amniotic uid biochemical parameters, erythrocyte morphology, oxidative stress and histopathological lesions [3][4][5][6][7] . As well as in the evaluation methods and collected materials, there is much variation in the ages and inoculation routes, among which the shell membrane, the allantoid uid, the chorioallantoic membrane (CAM), the yolk sac and the amnion are widely used [7][8][9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Physiological Changes in Chicken Embryos Inoculated with Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Sommerfeld

Mundim

Silva

et al. 2021

Preprint

View full text Add to dashboard Cite

Several works have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardise the evaluated parameters are needed to better understand and identify the viability of chicken embryos (CE) as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are peculiar to the model. To achieve this goal, in ovo assays were performed injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult chickens and other animals. Embryo response to virus and drug challenges may not occur as expected for adult chickens and even different species. While macroscopic and microscopic damage was visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not result in the expected effects for CE. On the other hand, with dexamethasone (DX), changes in blood parameters and biomarkers were inherent to the model and depended on the stage of CE development. Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are recorded.

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A new standardization for the use of chicken embryo: selection of target from the phage display library and infection

de Souza,

Sommerfeld,

Almeida-Souza

et al. 2024

Appl Microbiol Biotechnol

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The filamentous bacteriophage M13KO7 (M13) is the most used in phage display (PD) technology and, like other phages, has been applied in several areas of medicine, agriculture, and in the food industry. One of the advantages is that they can modulate the immune response in the presence of pathogenic microorganisms, such as bacteria and viruses. This study evaluated the use of phage M13 in the chicken embryos model. We inoculated 13-day-old chicken embryos with Salmonella Pullorum (SP) and then evaluated survival for the presence of phage M13 or E. coli ER2738 (ECR) infected with M13. We found that the ECR bacterium inhibits SP multiplication in 0.32 (M13-infected ECR) or 0.44 log UFC/mL (M13-uninfected ECR) and that the ECR-free phage M13 from the PD library can be used in chicken embryo models. This work provides the use of the chicken embryo as a model to study systemic infection and can be employed as an analysis tool for various peptides that M13 can express from PD selection. Key points • SP-infected chicken embryo can be a helpful model of systemic infection for different tests. • Phage M13 does not lead to embryonic mortality or cause serious injury to embryos. • Phage M13 from the PD library can be used in chicken embryo model tests.

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Hybrid Pectin-Liposome Formulation against Multi-Resistant Bacterial Strains

Cited by 23 publications

References 53 publications

Physiological Changes in Chicken Embryos Inoculated With Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Physiological Changes in Chicken Embryos Inoculated With Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Physiological Changes in Chicken Embryos Inoculated with Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

A new standardization for the use of chicken embryo: selection of target from the phage display library and infection

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