Hybrid Seed Production in<i>Capsicum</i>

Berke, Terry

doi:10.1300/j153v01n03_02

Cited by 24 publications

(17 citation statements)

References 20 publications

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“…According to different authors (Azurdia, 1995;Berke, 2000;Barrios et al, 2007), the stigma position at the anthers level facilitates self-pollination, whereas when the stigma is excerpt the percentage of cross-pollination increases. Such is the case of creole pepper morphotypes (C. annuum) collected in Yucatan that commonly show an excerpt stigma, which has favored cross-pollination and therefore the formation of new variants or hybrids is frequent (Latournerie, 2001).…”

Section: Resultsmentioning

confidence: 99%

Caracterización morfológica de híbridos de chile manzano

Arias

Carranza

Estrada

et al. 2017

Remexca

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ResumenEl chile manzano (Capsicum pubescens R y P) cultivado en el centro de México presenta variabilidad genética aprovechable en programas de mejoramiento genético. El objetivo de la investigación fue evaluar dos métodos de hibridación y caracterizar morfológicamente los descendientes F1 de las cruzas entre morfotipos de chile manzano. El experimento se desarrolló de 2012 a 2013 en invernadero con los progenitores identificados como flor blanca (FB), Flor morada-entrenudo largo (FML) y flor morada-entrenudo corto (FMC). Se hibridaron por los métodos "sin corola y "con corola" y la F1 se evaluó a prendimiento de fruto y caracterización morfológica. Los resultados indicaron que la protección de la flor hibridada afectó el prendimiento de fruto en más del 50%. No hubo diferencias significativas entre cruzas directas ni recíprocas (t; p= 0.42), lo que sugiere ausencia de efectos maternos. La posición del estilo tanto en progenitores como en los híbridos fue heteromórfica, con 70-90% al nivel de las anteras. Los variantes de los caracteres color del tallo, color de la corola, color del filamento y color del estilo se encontraron en el progenitor FB y fueron recesivos a los correspondientes de FML y FMC. El porte bajo de la planta AbstractThe manzano pepper (Capsicum pubescens R and P) cultivated in central México shows genetic variability that can be used in breeding programs. The purpose of this research was to evaluate two hybridization methods and to morphologically characterize F1 descendants of crosses between Manzano pepper morphotypes. The experiment was carried out from 2012 to 2013 in greenhouse with the parents identified as white flower (FB), purple flower-long internode (FML) and purple flower-short internode (FMC). They were hybridized by the "with corolla" and "without corolla" methods and the F1 was evaluated for fruit picking and morphological characterization. The results indicated that the protection of the hybridized flower affected fruit picking by more than 50%. There were no significant differences between direct or reciprocal crosses (t; p= 0.42), suggesting no maternal effects. The position of the style in both progenitors and hybrids was heteromorphic, with 70-90% at the anther level. The variants of the characters stem color, corolla color, filament color and style color were found in the FB progenitor and were recessive to the corresponding FML and FMC. The low plant size and smaller leaves present in the FB and FMC progenitors were dominant in826 Rev. Mex. Cienc. Agríc. Vol.8 Núm. 4 16 de mayo -29 de junio, 2017 Brenda Ayala Arias et al.y hojas de menor tamaño presentes en los progenitores FB y FMC fueron dominantes en los híbridos y afectaron negativa y significativamente en el rendimiento de frutos y heterosis. Los resultados sugieren estrategias de mejoramiento genético adicionales en híbridos como selección de segregantes y retrocruzas que mejorar el tamaño del fruto.Palabras claves: Capsicum pubescens, hibridización, morpotipos, variación genética. IntroducciónEl chile manzano (Caps...

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Section: Resultsmentioning

confidence: 99%

Caracterización morfológica de híbridos de chile manzano

Arias

Carranza

Estrada

et al. 2017

Remexca

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“…Cabai merupakan tanaman asli daerah tropis yang berasal dari Amerika Selatan dan Amerika Tengah, kemudian menyebar ke seluruh dunia (Berke, 2002). Tanaman cabai termasuk famili Solanaceae yang memiliki banyak jenis dan varietas, tetapi yang umum dibudidayakan untuk konsumsi adalah cabai besar, cabai keriting, cabai rawit, dan paprika (Wiryanta, 2002).…”

Section: Pendahuluanunclassified

Induksi Kalus serta Regenerasi Tunas dan Akar Cabai melalui Kultur In Vitro

Manzila¹,

Hidayat²,

Mariska³

et al. 2016

J. AgroBiogen

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Callus Induction and Regeneration of Shoot and Root ofChill through In Vitro Culture. Ifa Manzila, Sri H. Hidayat, Ika Mariska, and Sriani Sujiprihati. In vitro culture is one way for a fast and effective plant propagation. This method is also useful for preliminary selection of plant resistance to disease, including the chili. In vitro propagation method for chili has not been widely reported. A study was conducted to obtain effective techniques for callus induction and regeneration into shoots on three red chili cultivars (cv) Gelora, Sudra, and Chili 109. The study consisted of four activities, namely the induction of callus formation, induction of embryogenic callus, callus regeneration into adventitious shoots, and root induction from the adventitious shoots. Murashige Skoog (MS) medium + 0.6% agar + 3% sucrose were used as basal medium, 20 ml/bottle. Young leaves, hypocotyls and root tips of 21-day-old chili seedlings were used as sources of explants. Each experiment was arranged in a completely randomized design with 10 replications, one culture bottle for each treatment. The callus induction experiments using the explants of young leaf explants, hypocotyl, and root tips were done separately. Each treatment consisted of explants from the three chili cultivars on MS medium containing three composition of growth regulators (PGR) BAP + NAA, 10 explants/bottle. The embryogenic callus induction was conducted by growing the callus in bottles containing a medium that contains three compositions PGR 2,4-D + thidiazuron 0.5 mg/l. Induction of shoot formation was done by growing the embryogenic callus on medium containing three composition of plant growth regulator BAP + NAA. Induction of root formation was performed by growing adventitious shoots on ½ MS and 1 MS medium + NAA 0.5 to 1.0 mg/l. The results showed that young leaves are the best explant source for callus and shoot formations in chili through tissue culture compared with the hypocotyl and the tip. Gelora is the most responsive chili cultivar to callus, shoots, and roots formation of in their respective medium, compared to Sudra and Chile 109. MS medium containing BAP 3-7 mg/ml and NAA 1 mg/ml can be used to induce the growth of callus from young leaf explants, hypocotyl and seedling root tip chili cv Gelora, Sudra, and Chile 109, but its growth was very slow and did not produce embryogenic callus. Embryogenic callus formation can be induced by both non-embryogenic callus Hak Cipta © 2010, BB-Biogen growing the callus on MS medium containing 2,4-D 3 mg/l + thidiazuron 0.5 mg/ l. Formation of callus that can regenerate into shoots should use an MS medium containing 2,4-D 3 mg/l + thidiazuron 0.5 mg/ l followed by subculture on MS medium + BAP 3 mg/l + thidiazuron 0.5 mg/l to induce shoot elongation. Medium ½ MS and 1 MS containing NAA 0.5-1.0 mg/l can be used to induce root formations on shoot culture of chili cv Gelora but not for cv Chili 109.

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“…Seed maturation takes 25-85 days, depending on the genotype and the environmental conditions (Berke 2000). In the present study, seeds [immature, starting at 0 day post-anthesis (DPA), 18 days post-inoculation (DPI)] were collected from the self-pollinated flowers on the fourth branching node of PMMoV-infected C. annuum cv.…”

Section: Sampling and Detection Of Pmmov Around Seeds After Anthesismentioning

confidence: 99%

Immunolocalization of Pepper mild mottle virus in developing seeds and seedlings of Capsicum annuum

Genda¹,

Sato²,

Nunomura³

et al. 2011

J Gen Plant Pathol

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The location of Pepper mild mottle virus (PMMoV) within seeds as they developed on inoculated seedlings of pepper (Capsicum annuum) was followed over time by detecting the viral coat protein using immunofluorescence microscopy. Seedlings were inoculated with PMMoV when the flower buds on the first and second branching nodes were in bloom. Fluorescence indicating the presence of PMMoV was first observed around immature seeds and placentas in the ovaries on the fourth branching node at 20 days post-anthesis (20 DPA), which corresponded to 39 days post-inoculation (39 DPI). The area with fluorescence gradually expanded from the placenta into the integument and the parenchyma, and finally reached the tip of the immature seeds by 34 DPA (53 DPI). The embryo or endosperm beyond the endothelium never fluoresced during the experiment [i.e., ending at 81 DPA (102 DPI)]. For visualizing viral routes of invasion from seeds into new seedlings, PMMoV-infected C. annuum seeds that were heterozygous for the L 3 tobamovirus-resistance gene were sown in soil at 30°C. After *2 weeks, the cotyledon developed virally induced necrosis. These findings shed light on the infection cycle of PMMoV through vertical transmission in C. annuum.

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Hybrid Seed Production inCapsicum

Cited by 24 publications

References 20 publications

Caracterización morfológica de híbridos de chile manzano

Caracterización morfológica de híbridos de chile manzano

Induksi Kalus serta Regenerasi Tunas dan Akar Cabai melalui Kultur In Vitro

Immunolocalization of Pepper mild mottle virus in developing seeds and seedlings of Capsicum annuum

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