Hybrid spheroid microscaffolds as modular tissue units to build macro-tissue assemblies for tissue engineering

Guillaume, Olivier; Kopinski-Grünwald, Oliver; Weisgrab, Gregor; Baumgartner, Theresia; Arslan, Aysu; Whitmore, Karin; Vlierberghe, Sandra Van; Ovsianikov, Aleksandr

doi:10.1016/j.actbio.2022.03.010

Cited by 25 publications

(29 citation statements)

References 48 publications

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“…[33] ASCs Human Scaffold, Hydrogel When adipose-derived stem cell spheroids are cultured within BBs, the spheroids retain their differentiation potential. [34] MSCs Human Pellet culture…”

Section: Mscsmentioning

confidence: 99%

“…These authors proposed that heparin’s chondrogenic activity is due to the intrinsic nature of heparin, which secures endogenous growth factor (i.e., TGF-β) secretion from cells, which can enhance chondrogenesis. Guillaume et al [ 34 ] proved for the first time that the presence of a micro scaffold has no effect on the ability of cells to form spheroids or their viability. The study also revealed that the design of the micro scaffolds was chosen on the basis of the chemical structure of fullerene, known as buckyball (BB), because it has a spherical structure and is highly porous with thin struts (i.e., 35 m), which allows a cell suspension to form a spheroid within its core.…”

Section: Application Of 3d Spheroid Culture System To Chondrogenic Di...mentioning

confidence: 99%

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3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

Lee

2022

Life

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Cartilage is a connective tissue that constitutes the structure of the body and consists of chondrocytes that produce considerable collagenous extracellular matrix and plentiful ground substances, such as proteoglycan and elastin fibers. Self-repair is difficult when the cartilage is damaged because of insufficient blood supply, low cellularity, and limited progenitor cell numbers. Therefore, three-dimensional (3D) culture systems, including pellet culture, hanging droplets, liquid overlays, self-injury, and spinner culture, have attracted attention. In particular, 3D spheroid culture strategies can enhance the yield of exosome production of mesenchymal stem cells (MSCs) when compared to two-dimensional culture, and can improve cellular restorative function by enhancing the paracrine effects of MSCs. Exosomes are membrane-bound extracellular vesicles, which are intercellular communication systems that carry RNAs and proteins. Information transfer affects the phenotype of recipient cells. MSC-derived exosomes can facilitate cartilage repair by promoting chondrogenic differentiation and proliferation. In this article, we reviewed recent major advances in the application of 3D culture techniques, cartilage regeneration with stem cells using 3D spheroid culture system, the effect of exosomes on chondrogenic differentiation, and chondrogenic-specific markers related to stem cell derived exosomes. Furthermore, the utilization of MSC-derived exosomes to enhance chondrogenic differentiation for osteoarthritis is discussed. If more mechanistic studies at the molecular level are conducted, MSC-spheroid-derived exosomes will supply a better therapeutic option to improve osteoarthritis.

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“…[33] ASCs Human Scaffold, Hydrogel When adipose-derived stem cell spheroids are cultured within BBs, the spheroids retain their differentiation potential. [34] MSCs Human Pellet culture…”

Section: Mscsmentioning

confidence: 99%

Section: Application Of 3d Spheroid Culture System To Chondrogenic Di...mentioning

confidence: 99%

3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

Lee

2022

Life

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“…Due to the variable and complex shape and size of cartilage defects. An important benefit of microscaffold-based assembly units is the better control of size observed in the formation of larger tissue structures . Microswimmers can take advantage of size and reach the target site for assembly under the control of an external magnetic field multiple times.…”

Section: Resultsmentioning

confidence: 99%

“…Scaffolds with porous structures can protect the MSCs during injection and facilitate cell adhesion, growth, and migration; owing to these benefits, the implantation of scaffolds to repair cartilage has become a major treatment option for intra-articular cartilage defects. , Recently, the implantation of small-scale scaffolds called microscaffolds is emerging as a significant treatment alternative ,, because they can penetrate deep into cartilage defects more effectively than millimeter/centimeter-sized scaffolds. , MSCs cultured inside the microscaffolds retain denseness and differentiation potential. In addition, microscaffolds can be used as building blocks for tissue assembly, a potential modular tool for tissue engineering and regenerative medicine . Nevertheless, it remains a great challenge to target narrow or complex defects because microscaffolds cannot actively spread into the articular cavity and migrate to the site of cartilage defects.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, microscaffolds can be used as building blocks for tissue assembly, a potential modular tool for tissue engineering and regenerative medicine. 13 Nevertheless, it remains a great challenge to target narrow or complex defects because microscaffolds cannot actively spread into the articular cavity and migrate to the site of cartilage defects. To address this issue, researchers in the field of microrobotics attempted to use magnetic actuation to complement the targeting and migration shortcomings of microscaffolds.…”

Section: ■ Introductionmentioning

confidence: 99%

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2D Magnetic Microswimmers for Targeted Cell Transport and 3D Cell Culture Structure Construction

Chen

Song

et al. 2023

ACS Appl. Mater. Interfaces

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Cell delivery using magnetic microswimmers is a promising tool for targeted therapy. However, it remains challenging to rapidly and uniformly manufacture cell-loaded microswimmers that can be assembled into cell-supporting structures at diseased sites. Here, rapid and uniform manufacturable 2D magnetic achiral microswimmers with pores were fabricated to deliver bone marrow mesenchymal stem cells (BMSCs) to regenerate articular-damaged cartilage. Under actuation with magnetic fields, the BMSC-loaded microswimmers take advantage of the achiral structure to exhibit rolling or swimming motions to travel on smooth and rough surfaces, up inclined planes, or in the bulk fluid. Cell viability, proliferation, and differentiation tests performed days after cell seeding verified the microswimmers' biocompatibility. Long-distance targeting and in situ assemblies into 3D cell-supporting structures with BMSCloaded microswimmers were demonstrated using a knee model and U-shaped wells. Overall, combining the advantages of preparing an achiral 2D structured microswimmer with magnetically driven motility results in a platform for cell transport and constructing 3D cell cultures that can improve cell delivery at lesion sites for biomedical applications.

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Multiphoton Lithography as a Promising Tool for Biomedical Applications

Gréant

Durme

Hoorick

et al. 2023

Adv Funct Materials

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Multiphoton lithography (MPL) is a powerful and useful structuring tool capable of generating 2D and 3D arbitrary micro‐ and nanometer features of various materials with high spatial resolution down to nm‐scale. This technology has received tremendous interest in tissue engineering and medical device manufacturing, due to its ability to print sophisticated structures, which is difficult to achieve through traditional printing methods. Thorough consideration of two‐photon photoinitiators (PIs) and photoreactive biomaterials is key to the fabrication of such complex 3D micro‐ and nanostructures. In the current review, different types of two‐photon PIs are discussed for their use in biomedical applications. Next, an overview of biomaterials (both natural and synthetic polymers) along with their crosslinking mechanisms is provided. Finally, biomedical applications exploiting MPL are presented, including photocleaving and photopatterning strategies, biomedical devices, tissue engineering, organoids, organ‐on‐chip, and photodynamic therapy. This review offers a helicopter view on the use of MPL technology in the biomedical field and defines the necessary considerations toward selection or design of PIs and photoreactive biomaterials to serve a multitude of biomedical applications.

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Hybrid spheroid microscaffolds as modular tissue units to build macro-tissue assemblies for tissue engineering

Cited by 25 publications

References 48 publications

3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

2D Magnetic Microswimmers for Targeted Cell Transport and 3D Cell Culture Structure Construction

Multiphoton Lithography as a Promising Tool for Biomedical Applications

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