Hybrid Systems Biomolecule-Polymeric Nanoparticle: Synthesis, Properties and Biotechnological Applications

Palocci, Cleofe; Chronopoulou, Laura

doi:10.1007/978-90-481-3192-1_5

Cited by 2 publications

(1 citation statement)

References 239 publications

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“…Moreover, the hydrophilic shell can be decorated by pilot molecules, so allowing for targeted delivery. Among the commonly used synthetic polymers, poly(lactide -co- glycolide) (PLGA) has received considerable attention , because it combines biocompatibility and degradability with a degradation rate easily tuned by the copolymer composition [i.e., lactide (LA)/glycolide (GA) ratio]. Nevertheless, to stabilize PLGA nanocarriers in the body fluids and to increase the circulation time after injection in the bloodstream, the surface of the carrier can be covered and shielded by poly(ethylene oxide) (PEO) chains .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Poly(lactide-co-glycolide-co-ε-caprolactone)-graft-mannosylated Poly(ethylene oxide) Copolymers by Combination of “Clip” and “Click” Chemistries

et al. 2012

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Poly(lactide-co-glycolide) (PLGA) is extensively used in pharmaceutical applications, for example, in targeted drug delivery, because of biocompatibility and degradation rate, which is easily tuned by the copolymer composition. Nevertheless, synthesis of sugar-labeled amphiphilic copolymers with a PLGA backbone is quite a challenge because of high sensitivity to hydrolytic degradation. This Article reports on the synthesis of a new amphiphilic copolymer of PLGA grafted by mannosylated poly(ethylene oxide) (PEO). A novel building block, that is, α-methoxy-ω-alkyne PEO-clip-N-hydroxysuccinimide (NHS) ester, was prepared on purpose by photoreaction of a diazirine containing molecular clip. This PEO block was mannosylated by reaction of the NHS ester groups with an aminated sugar, that is, 2-aminoethyl-α-d-mannopyroside. Then, the alkyne ω-end-group of PEO was involved in a copper alkyne- azide coupling (CuAAC) with the pendent azides of the aliphatic copolyester. The targeted mannose-labeled poly(lactide-co-glycolide-co-ε-caprolactone)-graft-poly(ethylene oxide) copolymer was accordingly formed. Copolymerization of d,l-lactide and glycolide with α-chloro-ε-caprolactone, followed by substitution of chlorides by azides provided the azido-functional PLGA backbone. Finally, micelles of the amphiphilic mannosylated graft copolymer were prepared in water, and their interaction with Concanavalin A (ConA), a glyco-receptor protein, was studied by quartz crystal microbalance. This study concluded to the prospect of using this novel bioconjugate in targeted drug delivery.

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Section: Introductionmentioning

confidence: 99%