Hybrid Tea-rose pollen. II. Inheritance of pollen viability

Visser, T.; Vries, D.P. de; Scheurink, J. A. M.; Welles, G.W.H.

doi:10.1007/bf00021698

Cited by 11 publications

(5 citation statements)

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“…Company breeding databases have never been studied to assess fertility using pollen characteristics in roses. In previous studies to evaluate fertility, it has been commonplace to work with a small number of parents (Visser et al 1977b;de Vries and Dubois 1987;Ogilvie et al 1991;Crespel et al 2006). Our breeding database analysis enabled the selection of genotypes that are accurately characterised by high or low fertility in terms of seeds produced per hybridisation.…”

Section: Discussionmentioning

confidence: 99%

“…The integration of this knowledge on pollen quality increased the number of seedlings obtained per hybridisation by more than 200% (Gudin 1995). Visser et al (1977b) observed that pollen viability of hybrid tea roses is influenced by the season and is positively correlated to seedling production. Gudin et al (1991a) observed seasonal influence on pollen fertility and also on pollen size.…”

Section: Introductionmentioning

confidence: 99%

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Pollen morphology as fertility predictor in hybrid tea roses

Pipino

Labeke

Mansuino³

et al. 2010

Euphytica

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Fertility of hybrid tea roses is often reduced due to their interspecific origin but also to intensive inbreeding. New genotypes used as pollen donors represent an economic risk for a breeding programme, as their influence on seed production is unknown. In this study 11 cut rose genotypes were selected from a company database as high fertile or low fertile male parents, according to the number of seeds per hybridisation. Pollen morphology and in vitro germination of the selected genotypes were characterised. Pollen was either small (mean diameter \30 lm), shrunken, and irregular (abnormal), or large (mean diameter [30 lm), elliptical and crossed by furrows (normal). High correlations were found between the number of seeds produced per hybridisation and the pollen diameter (r = 0.94) or the percentage of normal pollen (r = 0.96). In order to evaluate the predictive power of the models, we conducted regression analyses and performed a validation experiment on genotypes not present in the database and without background information on fertility. Pollen diameter and percentage of normal pollen were characterised and fitted in the regression models for seed set predictions. Validation with an independent dataset gave a good prediction for 83.3% of the data. This indicates that using either the mean pollen diameter or the percentage of normal pollen resulted in effective fertility prediction. This tool could enhance the genetic variability in crossings between hybrid tea roses, thus creating possibilities for less economically risky exploitation of new tetraploid genotypes as male parents.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pollen morphology as fertility predictor in hybrid tea roses

Pipino

Labeke

Mansuino³

et al. 2010

Euphytica

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“…Finally it should be noted that pollen grain abortion commonly occurs in rose varieties (see also VISSER et al ., 1977) . The efficiency of germination, staining or storage methods is therefore best assessed in vitro by the percentage of normal pollen grains that are found viable .…”

Section: Discussionmentioning

confidence: 99%

Hybrid Tea-rose pollen. I. Germination and storage

Visser¹,

Vries²,

Welles³

et al. 1977

Euphytica

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Germination and storage trials were carried out with pollen of several rose varieties . The pollen grains germinated well in a 15%, sucrose solution with 40 ppm boric acid . Staining the pollen with a 0 .1 tetrazolium solution and standardizing the degree of colour at which the pollen grains are counted as viable, provided a good viability estimate, simpler to carry out than in vitro germination . Germination capacity and staining ability of the pollen were greatly impeded -about halved -by dehydration during storage in desiccators at low humidity . This effect could be corrected by humidifying the pollen beforehand for about one hour, though this pre-treatment increased the percentage of germinated pollen grains more than the percentage stained . There was no difference between the two percentages in fresh or in deep-frozen pollen .Pollen stored at 1 °C and high relative humidity soon lost its germination capacity ; between 0 and 20°" humidity a considerable proportion of the pollen remained viable for 9 months and longer . Storage for the same period in vacuum-sealed glass tubes at -24°C maintained viability as well or better and would probably prolong it further . Some of the cold-stored pollen induced a reasonable seed set after one year, a low seed set was obtained even after two years of storage at I °C and low humidity .

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“…All observations of slides were carried out at x 100 magnification using a grid mounted in the eyepiece of a Leitz Wetzlar photomicroscope. Following Visser et al (13,14), absolute pollen via bility, or the effective germination capacity in vivo, was calculated for fresh 1980 pollen samples using the formula. : % normal pollen x % pollen viability 100…”

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confidence: 99%

Pollen Viability in Rosa

Pearson¹,

Harney

1984

horts

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The viability of fresh pollen from 6 Rosa species (Rosa spinosissima L., R. fedtschenkoana Regel, R. pendulina L., R. arkansana Porter, R. Carolina L., R. virginiana Miller), one botanical variety (R. spinosissima altaica Rehd.) and 3 cultivars (‘Betty Bland’, ‘George Will’, ‘Prairie Princess’) were evaluated. Correlation between pollen staining and germination was positive and significant, but absolute pollen viability was found to be a better indicator of viability than staining. Rosa spinosissima and R. fedtshenkoana were considered to be the best pollen parents from the genotypes sampled.

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Hybrid Tea-rose pollen. II. Inheritance of pollen viability

Cited by 11 publications

References 1 publication

Pollen morphology as fertility predictor in hybrid tea roses

Pollen morphology as fertility predictor in hybrid tea roses

Hybrid Tea-rose pollen. I. Germination and storage

Pollen Viability in Rosa

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