Hybridization and in vitro culture of an orchid hybrid Ascocenda ‘Kangla’

Kishor, Rajkumar; Khan, Patan Shaik Sha Valli; Sharma, G. J.

doi:10.1016/j.scienta.2005.12.004

Cited by 33 publications

(20 citation statements)

References 7 publications

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“…Orchid hybrids made up majority of the commercial orchids in Malaysia and Singapore. There are lots of reasons that led to the wide area coverage of orchid hybrids cultivation, such as the ease of cultivation, free blooming habit, and compactness and fantastic arrays of shapes, colours and flowers (Kishor et al, 2006).…”

Section: Orchidaceaementioning

confidence: 99%

Discovering Fragrance Biosynthesis Genes from Vanda Mimi Palmer Using the Expressed Sequence Tag (EST) Approach

Teh¹,

Abdullah²,

Namasivayam³

et al. 2012

Data Mining Applications in Engineering and Medicine

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Section: Orchidaceaementioning

confidence: 99%

Discovering Fragrance Biosynthesis Genes from Vanda Mimi Palmer Using the Expressed Sequence Tag (EST) Approach

Teh¹,

Abdullah²,

Namasivayam³

et al. 2012

Data Mining Applications in Engineering and Medicine

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“…The orchids have highlighted acceptance on the ornamental flowers market (Kishor et al 2006;Ferreira et al 2016) and to meet the growing commercial demand, the main way to produce their seedlings is through in vitro propagation (Rego-Oliveira and Faria 2005;Sharma 2016), a method that provides a large number of seedlings, in reduced space and time, economically viable.…”

Section: Introductionmentioning

confidence: 99%

Chemical Disinfestation for Sowing and in Vitro Re-cultivation of Orchids and its Influence on the Seedlings Nutrition

Fontes¹,

Álvarez²,

Novais³

et al. 2017

JAS

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The use of a commercial bleach solution (CB) for in vitro propagation of orchids has proven to be an efficient technique and an alternative to the use of the autoclave and laminar flow chamber (LFC). The aim of this study was to evaluate the use of CB in disinfestation of culture media (CM) for seeding and for re-cultivation of orchid plantlets and its effect in different types of flasks and on the nutritional state of the plantlets. In the seeding experiment, seeds of Laelia tenebrosa Rolfe were used, with evaluation of three types of flasks; five concentrations of CB applied to the CM of 2, 5, 10, 20 and 40 mL L -1 and the control treatment. In the re-cultivation experiment, Cattleya kerri Brieger & Bicalho plantlets were used. The rates of CB added to the CM were 2, 5, 10, 20, 30, 35 and 38 mL L -1 in polypropylene containers, and the rates sprayed on the plantlets at re-cultivation were 5, 12.5, 25, 50, 75, 87.5 and 95 mL L -1 . The proportion of germinated seeds and the dry matter weight of the protocorms were greater in the control treatment. The increasing rates of CB added to the CM had worse results for germination in glass flasks. In re-cultivation, there was no significant difference in the growth of the plantlets between the autoclaved flasks and those disinfested in CB.Keymessage: Both orchid seeding and re-cultivation of seedlings can be carried out entirely and efficiently without the use of an autoclave and laminar flow chamber, using chemical disinfection with commercial bleach.

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“…The flowers exhibit an excessively wide range of variation in shape, form, size and colouration surpassing flowers of all the other Angiosperms. As a result, they are highly valued in floritrade as species or hybrids for pot plant or cut flower and holds enormous promise (Kishor et al, 2006). Endemic orchids of the world are facing the grim possibility of extinction under intense biotic pressures like forest fires, indiscriminate wild collection and illegal trade by people.…”

Section: Introductionmentioning

confidence: 99%

“…However, development of new hybrid orchid is a tedious work that calls for great patience. Breeding orchids for the synthesis of hybrids with superior characters was extensively performed during the last century (Kishor et al, 2006). Propagation through conventional method such as vegetative propagation is a very slow process.…”

Section: Introductionmentioning

confidence: 99%