Hybridization and its Application in Centrarchids

Shen, Zhigang; Wang, Hanping

doi:10.1002/9781119127291.ch19

Cited by 4 publications

(1 citation statement)

References 92 publications

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“…Changes in the water temperature result in various physiological and molecular stress responses to maintain the essential biological functions in fish and other aquatic species ( Eissa and Wang, 2013 , 2016 ; Eissa et al, 2017 , 2018 ). Bluegill sunfish ( Lepomis macrochirus ) belongs to Centrarchidae ( Near and Koppelman, 2009 ) and receives much attention because of the extraordinary biological characteristics, such as sex-determining mechanism ( Wang et al, 2014 ; Shen et al, 2016 ), hybridization ( Shen and Wang, 2018b ), and alternative mating tactics ( Gross and Charnov, 1980 ; Dominey, 1981 ; Garner and Neff, 2013 ). These biological features influence population sex ratio solely or interactively and make bluegill to be an excellent organism for investigation of interactions, population dynamics, speciation, and sexual selection.…”

Section: Introductionmentioning

confidence: 99%

Effects of Temperature on the Expression of Two Ovarian Differentiation-Related Genes foxl2 and cyp19a1a

et al. 2018

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Exposure to stress induces a series of responses and influences a wide range of biological processes including sex differentiation in fish. The present work investigated the molecular and physiological response to thermal stress throughout the early development stage covering the whole period of sex differentiation of bluegill, Lepomis macrochirus. Larvae were treated using three temperatures, 17, 24, and 32°C from 6 to 90 days posthatching (dph) in 30-L round tanks. There is no significant difference of the sex ratio and survival among the three temperature groups in the geographic population used in this study. Two ovarian differentiation-related genes foxl2 and cyp19a1a were detected at 7 dph suggesting that these genes have already played a role prior to sex differentiation. The expression of foxl2 reached the peak and was thermosensitive just prior to the onset of ovarian differentiation at 27 dph. Histological examination displayed that the proliferation of germ cells and ovarian differentiation were delayed at the low-temperature treatment (17°C) at 97 dph compared with higher temperatures. In conclusion, the water temperature regulates the sex differentiation of bluegill through modulation of the expression of foxl2 and cyp19a1a. A comparative study of the expression profile of sex differentiation-related genes in species will shed light on the evolution of sex-determination mechanisms and the impact of stress on sex differentiation.

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Section: Introductionmentioning

confidence: 99%

Effects of Temperature on the Expression of Two Ovarian Differentiation-Related Genes foxl2 and cyp19a1a

et al. 2018

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Effects of arginine family amino acids supplementation on growth, whole-body amino acid profiles, antioxidant capacity, and gene expression of juvenile largemouth bass (Micropterus salmoides)

Luan,

Li,

et al. 2025

Aquaculture

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Integrating ecological and molecular data to investigate species maintenance and interspecific hybridization between the redbreast sunfish (Lepomis auritus) and the pumpkinseed sunfish (Lepomis gibbosus)

et al. 2022

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True sunfishes (genus Lepomis) of North America are renowned for hybridization, although hybrids often comprise of only a fraction of the sunfish population in natural systems. Species boundaries may be maintained through premating mechanisms (e.g., ethological and habitat isolation), which may be facilitated by the promiscuous sunfish mating system; primarily, singular males guard nests which multiple females visit. Natural hybridization occurs between redbreast (L. auritus (Linnaeus, 1758)) and pumpkinseed (L. gibbosus (Linnaeus, 1758)) sunfishes, but there is no published research into its extent and causes. In this study, we assessed the relationship of ecological variables to the prevalence of hybridization in a sunfish population in a freshwater lake. Nests of both species were evaluated for differences in substrate, date, water depth, temperature, and dissolved oxygen. Sequencing of one fragment of nuclear DNA (calmodulin intron 4) and one fragment of mitochondrial DNA (12S) was employed on adult males and eight offspring from each nest to determine genetic identity and hybridization direction. Nonparametric tests found a significant difference in substrate between species’ nests (p < 0.01), warranting further investigation into nest substrate as a mechanism for species maintenance. Furthermore, we observed asymmetrical hybridization with the less abundant species (redbreast sunfish) disproportionately affected.

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Hybridization and its Application in Centrarchids

Cited by 4 publications

References 92 publications

Effects of Temperature on the Expression of Two Ovarian Differentiation-Related Genes foxl2 and cyp19a1a

Effects of Temperature on the Expression of Two Ovarian Differentiation-Related Genes foxl2 and cyp19a1a

Effects of arginine family amino acids supplementation on growth, whole-body amino acid profiles, antioxidant capacity, and gene expression of juvenile largemouth bass (Micropterus salmoides)

Integrating ecological and molecular data to investigate species maintenance and interspecific hybridization between the redbreast sunfish (Lepomis auritus) and the pumpkinseed sunfish (Lepomis gibbosus)

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