Hybridization chain reaction and DNAzyme-based dual signal amplification strategy for sensitive fluorescent sensing of aflatoxin B1 by using the pivot of triplex DNA

A highly sensitive competitive aptasensor for AFB1 detection based on an exonuclease-assisted target recycling amplification strategy

“…The chemiluminescence method 30 could detect 0.2 ng mL −1 of AFB 1 but the process was time-consuming and tedious. According to a recent report by Zou et al , 31 ultrasensitive performance with 0.066 ng mL −1 LOD of AFB 1 could be achieved by means of fluorescence, but the linear range was relatively narrow. The proposed aptasensor showed good detection capability of a wide linear range and high sensitivity, as well as simple operation and rapid analysis.…”

Organophosphonic Acid-Regulating Assembly of PV–SbIII Polyoxotungstate and Its Potential in Building a Dual-Signal Readout Electrochemical Aptasensor for Carcinogen Detection

“…POM-based electrochemical biosensors (PEBs) have aroused scientists’ attention in recent years. , However, the majority of reported PEBs are based on a single readout signal, which limits the detection precision to some extent. , For the purpose of further improving the accuracy of detection, we have built a POM-based dual-signal readout electrochemical aptasensor (DSREA) for the first time in the POM field, which can be used to reliably recognize aflatoxin B1 (AFB1). As AFB1 is widely known as the most dangerous carcinogen, reliable and sensitive biosensors for AFB1 are urgently needed. − …”

Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

“…Finally, based on network HCR, signal amplification is realized and sensitive detection of AFB 1 in peanut oil was realized with with LOD of 0.492 pg mL −1 and the linear range of 1–1000 pg mL −1 . In the other report, Zuo et al [ 237 ] combined DNAzyme with substrate chain (Zn-Sub) and enzyme chain (Zn-Enz) with HCR products to form a Y-shaped structure, which can significantly enhance the fluorescence intensity of the detection target. The fluorescent aptamer sensor proposed by researchers shows excellent performance with LOD of 0.22 nmol L −1 and the linear range of 0.4–16 nmol L −1 .…”