Hybridization chain reaction (HCR) for amplifying nanopore signals

Sun, Hong; Yao, Fei; Su, Zhuoqun; Kang, Xiaofeng

doi:10.1016/j.bios.2019.111906

Cited by 45 publications

(21 citation statements)

References 39 publications

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“…This assay achieved a desirable LOD of 30 fM which was much lower than that of conventional nanopores without amplification strategies. Sun et al fabricated an HCR-nanopore biosensor based on a polyethylene terephthalate (PET) membrane for circulating tumor DNA (ctDNA) detection, and the amplified nanopore signals exhibited a low LOD of 2.8 fM toward target ctDNA of KRAS G12DM. Moreover, Wu et al expanded the nanopore biosensor to detect tiny metal ions (Figure B).…”

Section: Hcr-based Biosensors With Nanomaterialsmentioning

confidence: 99%

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

et al. 2020

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With the continuous development of biosensors, researchers have focused increasing attention on various signal amplification strategies to pursue superior performance for more applications. In comparison with other signal amplification strategies, hybridization chain reaction (HCR) as a powerful signal amplification technique shows its certain charm owing to nonenzymatic and isothermal features. Recently, on the basis of conventional HCR, this technique has been developed and improved rapidly, and a variety of HCR-based biosensors with excellent performance have been reported. Herein, we present a systematic and critical review on the research progress of HCR in biosensors in the last five years, including the newly developed HCR strategies such as multibranched HCR, migration HCR, localized HCR, in situ HCR, netlike HCR, and so on, as well as the combination strategies of HCR with isothermal signal amplification techniques, nanomaterials, and functional DNA molecules. By illustrating some representative works, we also summarize the advantage and challenge of HCR in biosensors, and offer a deep discussion of the latest progress and future development trends of HCR in biosensors.

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Section: Hcr-based Biosensors With Nanomaterialsmentioning

confidence: 99%

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

et al. 2020

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“…For instance, Sun et al have applied the solid-state nanopore to detect ctDNA originating from serum samples. This strategy cooperates with the hybridization chain reaction to amplify the target's signals, improve data authenticity, and overcome the hurdles of nanopore application (76).…”

Section: Future Directions Improving the Limit Of Detection Of Ctdnamentioning

confidence: 99%

Application of Data Science in Circulating Tumor DNA Detection: A Promising Avenue Towards Liquid Biopsy

Xie

et al. 2021

Front. Oncol.

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The circulating tumor DNA (ctDNA), as a promising biomarker of liquid biopsy, has potential clinical relevance on the molecular diagnosis and monitoring of cancer. However, the trace concentration level of ctDNA in the peripheral blood restricts its extensive clinical application. Recently, high-throughput-based methodologies have been leveraged to improve the sensitivity and specificity of ctDNA detection, showing a promising avenue towards liquid biopsy. This review briefly summarizes the high-throughput data features concerned by current ctDNA detection strategies and the technical obstacles, potential solutions, and clinical relevance of current ctDNA profiling technologies. We also highlight future directions improving the limit of detection of ctDNA for better clinical application. This review may serve as a reference for the crosslinks between data science and ctDNA-based liquid biopsy, benefiting clinical translation in advanced cancer diagnosis.

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“…25 The signal amplication mechanism in this work is the hybridization chain reaction, and its function is to amplify the target signal in an isothermal environment in an enzyme-free manner. 26,27 Its working mechanism is that if the H1 chain is opened by a DNA trigger, this unfurled chain can open the H2 chain, which in turn can be hybridized with H1, triggering a cascading reaction. 28,29 When the two hairpins in the reaction system are completely consumed and an alternating polymer is produced, 30 the reaction stops.…”

Section: Introductionmentioning

confidence: 99%