Hybridization chain reactions on silica coated Qbeads for the colorimetric detection of multiplex microRNAs

Abstract

A robust Qbead platform that integrated with target-binding, hybridization chain reaction and staining was developed for the direct multiplexed detection of endogenous miRNAs by amplified Qbead-colour change.

Label-Free Platform for MicroRNA Detection Based on the Fluorescence Quenching of Positively Charged Gold Nanoparticles to Silver Nanoclusters

“…The detection performance of the present work was compared with literatures based on the assessment of sensitivity and specificity, and the results are listed in Table . Taking advantage of DSN-assisted target cycling, the detection of miRNA-155 was realized at femtomolar level with a detection limit of 33.4 fM, which was comparable with most reference works. ,,,,− Furthermore, the target miRNA-155 could be clearly discriminated from mutated sequences and other miRNAs without obvious interference from common metal ions and protein molecules, illustrating acceptable specificity of the strategy in contrast to reference methods. Therefore, the proposed system is promising for sensing applications.…”

“…Thus, effective detection of miRNAs is of great significance for clinical diagnosis and the research of pathogenesis . Recently, various intriguing sensing methods have been developed for miRNAs detection based on electrochemical, − colorimetric, − electrochemiluminescent, , fluorescent, − and chemiluminescent imaging detection platforms . Among these, the fluorescence-based detection system has been extensively employed in bioanalysis, due to its advantages of high sensitivity and selectivity, fast analysis, cost-effectiveness, and ease of operation. − …”

An enzyme-free molecular catalytic device: dynamically self-assembled DNA dendrimers for in situ imaging of microRNAs in live cells

“…7), a stronger uorescence signal could be observed because the expression level of miR-21 is higher than that of miR-155 in MCF -7 cells. 51,52 In addition, traditional CHA and direct FISH are also performed in which bCHA exhibits a stronger uorescence signal than CHA and direct FISH, demonstrating the proposed bCHA strategy can be used as a general platform for detecting various miRNA targets with high sensitivity and selectivity.…”

Homogeneous and Universal Detection of Various Targets with a Dual‐Step Transduced Toehold Switch Sensor

“…Therefore, various microRNAs have become very important biomarkers for disease diagnostics. MiRNA‐21, a microRNA highly expressed in many cancers, was taken as the model . Because miRNA‐21 only has 21 nt, it would be hard to design an effective toehold switch directly according to its own sequence.…”