Hybridization induced ion-barrier effect for the label-free and sensitive electrochemical sensing of Hepatocellular Carcinoma biomarker of miRNA-122

Gao, Feng; Chu, Yaru; Ai, Yijing; Yang, Weiqiang; Lin, Zhenyu; Wang, Qingxiang

doi:10.1016/j.cclet.2020.12.036

Cited by 23 publications

(13 citation statements)

References 27 publications

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“…The expression levels of circulating miRNAs can vary with disease states and can regulate disease development [2][3][4][5]. Reports have shown that circulating miRNAs are useful biomarkers for diseases as their presence in serum is relatively stable [6,7]. Therefore, accurate detection of disease-related circulating miRNAs is considered an attractive approach for the diagnosis and potentially the treatment of diseases.…”

Section: Introductionmentioning

confidence: 99%

Multistage nucleic acid amplification induced nano-aggregation for 3D hotspots-improved SERS detection of circulating miRNAs

et al. 2022

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Circulating miRNAs in the blood can regulate disease development and thus indicate disease states via their various expression levels. For these reasons, circulating miRNAs constitute useful biomarkers, and an approach to the accurate detection of circulating miRNAs is attractive in the diagnosis and treatment of diseases. However, methods for clinical detecting of circulating miRNA that take both sensitivity and practicality into account are still needed. Therefore, we aimed herein to solve some inherent problems in the actual detection using a robust surface-enhanced Raman scattering (SERS) platform with integrated nucleic acid amplification and nanoparticle aggregation to construct 3D hotspots for improving performance of analyzing circulating miRNAs. After target recognition and initial signal amplification by DNAzyme, we observed that release triggered an open hairpin DNA on gold nanoparticles (AuNPs), which then promote AuNP aggregation, causing the accumulation of a large number of hotspots in three-dimention. The SERS biosensor achieved a better performance than the sandwich-type separation detection, with a low detection limit (0.37 fM) and a broad linear range (1 fM–10 nM) in liquids. This SERS platform can be used as a powerful tool for the detection of circulating miRNAs, and it can be used to improve the sensitivity and accuracy of various clinical-disease diagnoses. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Multistage nucleic acid amplification induced nano-aggregation for 3D hotspots-improved SERS detection of circulating miRNAs

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Multistage Nucleic Acid Amplification Induced Nano-Aggregation for 3D Hotspots-Improved SERS Detection of Circulating miRNAs

Sun

Fang

Yang

et al. 2022

Preprint

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Circulating miRNAs in the blood can regulate disease development and thus indicate disease states via their various expression levels. For these reasons, circulating miRNAs constitute useful biomarkers, and an approach to the accurate detection of circulating miRNAs is attractive in the diagnosis and treatment of diseases. However, methods for detecting circulating miRNA that take both sensitivity and practicality into account are not currently available. Therefore, we aimed herein to solve some inherent problems in the actual detection using a robust surface-enhanced Raman scattering (SERS) platform with integrated nucleic acid amplification and nanoparticle aggregation to construct 3D hotspots for improving performance of analyzing circulating miRNAs. After target recognition and initial signal amplification by DNAzyme, we observed that release triggered an open hairpin DNA on gold nanoparticles (AuNPs), which then promote AuNP aggregation, causing the accumulation of a large number of hotspots in three-dimention. The SERS biosensor achieved a better performance than the sandwich-type separation detection, with a low detection limit (0.37 fM) and a broad linear range (1 fM-10 nM) in liquids. This SERS platform can be used as a powerful tool for the detection of circulating miRNAs, and it can be used to improve the sensitivity and accuracy of various clinical-disease diagnoses.

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“…Blood has long been considered as an optimal sample to monitor the physiological and pathological changes of the whole body. − As a form of “liquid biopsy”, protein tests on blood have received increasing attention for the discovery of disease biomarkers and monitoring of the therapeutic response to treatment. − Compared with single-plex immunoassays, multiplexing, highly specific, antibody-independent, and hypothesis-free mass spectrometry (MS)-based proteomic technology is the dominant method for high-throughput blood proteome profiling and biomarker screening. − Although great efforts have been devoted to discovering blood biomarkers to aid in early disease diagnosis and therapeutic response monitoring, few proteins have been successfully validated and have received FDA approval in recent years; thus, there is extensive research interest in developing new sample treatment and mass spectrometry (MS)-based methods for biomarker screening. ,− The major challenge in MS-based blood biomarker development is the highly complex nature of plasma/serum samples. Plasma/serum proteins have an extremely large abundance dynamic range that spans more than 10 orders of magnitude, with disease-derived proteins often present at the lower end of this range .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Surface-Functionalized Molybdenum Disulfide Nanomaterials for Efficient Adsorption and Deep Profiling of the Human Plasma Proteome by Data-Independent Acquisition

Liu

Yang

et al. 2022

Anal. Chem.

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Blood is one of the most important clinical samples for protein biomarker discovery, as it provides rich physiological and pathological information and is easy to obtain with low invasiveness. However, the discovery of protein biomarkers in the blood by mass spectrometry (MS)-based proteomic strategies has been shown to be highly challenging due to the particularly large concentration range of proteins and the strong interference by the high-abundant proteins in the blood. Therefore, developing sensitive methods for low-abundant biomarker protein identification is a key issue that has received great attention. Here, we report the synthesis and characterization of surface-functionalized magnetic molybdenum disulfide (MoS 2 ) for the large-scale adsorption of low-abundant plasma proteins and deep profiling by MS. MoS 2 nanomaterials resulted in the coverage of more than 3400 proteins (including a single-peptide hit) in a single LC−MS analysis without peptide prefractionation using pooled plasma samples, which were five times more than those obtained by the direct analysis of the plasma proteome. A detection limit in the low ng L −1 range was obtained, which is rare compared with previous reports.

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Hybridization induced ion-barrier effect for the label-free and sensitive electrochemical sensing of Hepatocellular Carcinoma biomarker of miRNA-122

Cited by 23 publications

References 27 publications

Multistage nucleic acid amplification induced nano-aggregation for 3D hotspots-improved SERS detection of circulating miRNAs

Multistage nucleic acid amplification induced nano-aggregation for 3D hotspots-improved SERS detection of circulating miRNAs

Multistage Nucleic Acid Amplification Induced Nano-Aggregation for 3D Hotspots-Improved SERS Detection of Circulating miRNAs

Synthesis of Surface-Functionalized Molybdenum Disulfide Nanomaterials for Efficient Adsorption and Deep Profiling of the Human Plasma Proteome by Data-Independent Acquisition

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