Hybridization of plant ribosomal RNA to DNA: the isolation of a DNA component rich in ribosomal RNA cistrons.

Matsuda, Kazutoshi; Siegel, Albert

doi:10.1073/pnas.58.2.673

Cited by 68 publications

(24 citation statements)

References 24 publications

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“…Essentially all of pumpkin nuclear DNA complementary to ribosomal RNA (rDNA) (2.2-2.4% of nuclear DNA) was found in a satellite (38,44) of similar density to that of the muskmelon satellite. Since pumpkin and muskmelon are in the same family, Cucurbitaceae, our satellite may contain a similar amount of rDNA.…”

Section: Discussionmentioning

confidence: 99%

Novel Properties of Satellite DNA from Muskmelon

Bendich¹,

Anderson²

1974

Proc. Natl. Acad. Sci. U.S.A.

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The purified dense satellite of muskmelon (Cucumis melo L.) represents 30% of the total DNA and forms a sharp, unimodal peak in both neutral and alkaline CsCl gradients. Spectrophotometric melting and DNA reassociation analyses revealed that one-third of the satellite is high melting and has a complexity of about 2.5 X 105 daltons, while the remaining two-thirds of the satellite melts 80 lower and has a complexity of about 5 X 108 daltons. The thermal stability of reassociated satellite DNA indicates that the multiple copies of the two melting fractions are essentially identical. The sharp, unimodal peak in Cs2SO4 becomes two distinct peaks when either silver or mercuric ions are included in the Cs2 S04 gradient One or more extremely rapidly reassociating components of nuclear DNA are present in many animals, often detectable as "satellite" bands in CsCl gradients (1). The kinetics of strand reassociation and the stability of these reformed duplexes are interpreted to mean that the multiple DNA segments are highly reiterated and are closely similar copies (2-4). Although its function is not known, some satellite DNA in mice (5) and insects (6, 7) hybridizes primarily with the centromeric regions of chromosomes in cytological preparations. It is enriched in heterochromatin of guinea pig (8), mouse, crab (9) and calf (10), and in mouse nucleoli (11). The mouse satellite does not appear to be transcribed (12). These unusual properties have led to the suggestion that satellite DNA may have a structural function in chromosome folding, pairing, and movement (12,13).From its reassociation kinetics, mouse satellite was estimated to contain about a million very similar copies comprising some 10% of the total mouse DNA (2, 14). The repeating unit in guinea pig a-satellite DNA has been estimated from oligonucleotide digestion products to be six nucleotides long (somewhat longer in the mouse) with about 12% nucleotide changes in the repeating units (15). Sequencing techniques have also provided length estimates of ten in a kangaroo rat satellite (16) and seven for each of three satellites in Drosophila virilis (17). The original complexity estimate (2) for mouse satellite of 300-400 nucleotide pairs (np) was considered too large after estimates were made of the marked influence of mispairing on reassociation rate (18,19

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Section: Discussionmentioning

confidence: 99%

Novel Properties of Satellite DNA from Muskmelon

Bendich¹,

Anderson²

1974

Proc. Natl. Acad. Sci. U.S.A.

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“…This suggests either that rRNA specificities are of little importance in general protein synthesis, or else that, at least in genera in which nucleolar suppression is widespread, the rRNAs of different species are similar. In this connection, Matsuda and Siegel (1967) showed that rRNAs from four plant species of four different families were remarkably similar to each other in hybridisation behaviour with several different plant DNAs.…”

Section: Discussionmentioning

confidence: 99%

Nucleolar suppression, its inheritance and association with taxonomy and sex in the genus Ribes

Keep¹

1971

Heredity

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“…DNA extracted from nuclei of C. pepo leaves displays a prominent dense rDNA containing satellite component (p = 1.706) upon isopycnic centrifugation (21). Material consisting largely of the satellite component was prepared, digested with restriction endonucleases, and subjected to agarose gel electrophoresis.…”

Section: Resultsmentioning

confidence: 99%

“…Nuclear DNA was isolated from young pumpkin (Cucurbita pepo L. cv Small Sugar) leaves (27) and fractionated by isopycnic centrifugation in CsCl. Preparations enriched in rDNA were obtained by recovering DNA from those fractions containing the dense satellite component (21).…”

Section: Methodsmentioning

confidence: 99%

“…The present study was undertaken to reassess rDNA heterogeneity in a plant species with a relatively large number of repeat units. The organism chosen for study is Cucurbita pepo L. whose rDNA is contained in an easily fractionated dense isopycnic satellite component (21). The genomic repeat unit number has been estimated to be 3400 (30) with some variation possibly resulting from differences between cultivars (22).…”

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