Hybridization properties of ribosomal RNA from rabbit tissues

Partial sequence analysis of ribosomal ribonucleic acid from HeLa cells. IV. Comparison of the primary structures of 28S and 18S ribonucleic acid from HeLa cells and different human tissues

“…Gould et al (1966) analyzed by polyacrylamide gel electrophoresis the products of limited digestion by T1 ribonuclease of rRNA (unfractionated) prepared from different organisms and from two cell types, reticulocytes and liver cells, of the same organism (rabbit): while they found differences in the pattern of degradation of the rRNA from different organisms, the rRNA from the two rabbit cell types analyzed gave identical results. Similarly, RNA-DNA hybridization experiments failed to show any difference in sequence between high molecular weight rRNA components prepared from rabbit reticulocytes and liver (Di Girolamo et al, 1969), or from different developmental stages of sea urchin (Mutolo and Giudice, 1967). The above-mentioned negative results could conceivably reflect the insensitivity of the analytical methods employed.…”