Hybrids derived from the viruses of alastrim and rabbit pox

Bedson, H. S.; Dumbell, K. R.

doi:10.1017/s0022172400039875

Cited by 25 publications

(18 citation statements)

References 10 publications

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“…This finding is in great contrast with that of clones derived from the parent These findings may be best interpreted as that plaguing efficiency is controlled by multiple genes, and hence the genetic recombination between the two vaccinia viruses with different plaguing efficiencies for L cells results in recombinants with a variety of plaguing efficiency. Previous studies indicate ready occurrence of recombination between the members of the variolavaccinia group [1][2][3][4][5][6][7][8][9]12].…”

Section: Stability Of Properties Of Monotypic Clones From Mixed Infecmentioning

confidence: 99%

Genetic Recombination between Vaccinia Virus Strains Distinct in Hemagglutinin Production and Plaquing Efficiency

Ando

Matumoto

1968

Japanese Journal of Microbiology

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Oda (1965) reported the rescue of dermovaccinia (strain Dairen I) abortive infection by ncurovaccinia virus (strain IHD-T) in mouse fibroblastic L cells. The present study, however, provided no evidence for such, but gave evidence for the occurrence of genetic recombination between the viruses in L and HeLa cells. HeLa and L cells were infected simultaneously with strain Dairen I which produces hemagglutinin (H+) and replicates well in HeLa cells but only poorly in L cells, and strain IHD-T which is H-and multiplies well in both cells. Sixty-eight of 225 and 24 of 114 clone preparations from the mixed infection yields in L and HeLa cells, respectively, were mixtures of H+ and H-viruses. The remaining 157 and 90 clones contained only one type of virus, H+ or H-, and varied widely in the plaguing efficiency for L cells. This contrasted with the results from parent viruses. An interpretation is that the plaguing efficiency is controlled by multiple genes and genetic recombination between the two vaccinia viruses with differing plaguing efficiencies results in occurrence of recombinants demonstrating a variety of plaguing efficiency. Hemagglutinin production was an all-or-none character, no intermediates being found, and can readily be tested at the particle level by the plaque-hemadsorption technique. These points render hemagglutinin production as a suitable marker for the genetic studies in contrast to the polygenic plaguing efficiency.

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Section: Stability Of Properties Of Monotypic Clones From Mixed Infecmentioning

confidence: 99%

Genetic Recombination between Vaccinia Virus Strains Distinct in Hemagglutinin Production and Plaquing Efficiency

Ando

Matumoto

1968

Japanese Journal of Microbiology

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“…In answer to this question it may be said that all the arguments advanced in the case of the alastrim-rabbit pox (AR) hybrids (Bedson & Dumbell, 1964) apply with at least equal force to the VC viruses. Thus it is to be noted that 6 of the 10 new types of virus were developed from a single reactivation pock.…”

Section: Analysis Of Resultsmentioning

confidence: 99%

“…The isolation of presumptive hybrids from the viruses of variola major and cowpox has already been described (Dumbell & Bedson, 1964). We report here the detailed characterization of the 16 clones of virus obtained.…”

Section: Introductionmentioning

confidence: 94%

“…We report here the detailed characterization of the 16 clones of virus obtained. As with the alastrimrabbit pox hybrids considered in the previous paper (Bedson & Dumbell, 1964), attention has been paid to the behaviour of the ceiling temperature character in recombination and to the possible relationship it and other markers might have to the virulence of these viruses. Both sets of hybrids may also be regarded as making a minor contribution to pox virus genetics, in that variola viruses have now been shown to form hybrids with other members of the variola-vaccinia group.…”

Section: Introductionmentioning

confidence: 99%

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Hybrids derived from the viruses of variola major and cowpox

Bedson

Dumbell

1964

J. Hyg.

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The characterization of 16 clones of virus derived from mixed infections with variola major and cowpox has been described. This work has involved the description of the plaques produced by variola major in chick embryo monolayers, and a more detailed study of the effects of raised temperature on pock production by pox viruses on the C.A.M.Two of the variola major—cowpox clones were found to correspond to cowpox virus, while the other 14 shared various combinations of parental characters. Among them there were 10 distinct new types of virus. Evidence of the stability of these viruses has been presented.Analysis of the combination of characters encountered among the hybrids has given good evidence that all the eight marker characters used segregate independently. A particular instance of this was the character of chick embryo virulence which, among the hybrid viruses, was shown to be unrelated either to ceiling temperature or speed of plaque formation.The significance of these findings in relation to the general problem of virulence has been briefly discussed. The possibility that vaccinia may have originated as a hybrid of variola and cowpox has lso been considered.

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“…Those cowpox-variola recombinants which had 'd' substance (VC 2,5,7,13,14) all gave intermediate pocks, described by Bedson & Dumbell (1964b) as having some degree of central haemorrhage, while those with no detectable 'd' (VC 6,8,10,12,16) gave uniformly white pocks. The two variola-rabbitpox recombinants (AR 1, 2) which had 'd' gave very small white pocks without any haemorrhage, but these two viruses also gave rabbit skin lesions which were necrotic but not haemorrhagic (Bedson & Dumbell, 1964a).…”

Section: Discussionmentioning

confidence: 99%

A poxvirus antigen associated with pathogenicity for rabbits

Rondle

Dumbell

1982

J. Hyg.

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SummaryWhite pock variants of cowpox virus give papular lesions on intradermal inoculation of rabbits, without the necrosis and haemorrhage that are produced by wild type cowpox viruses. Rondle & Dumbell (1962) have shown that white pock variants of cowpox virus fail to produce a specific, precipitating antigen which they called ‘d’ substance. In this paper it is shown that ‘d’ is demonstrable in soluble antigen preparations of rabbitpox virus and of neurovirulent strains of vaccinia virus but not in soluble antigens of variola viruses. Two series of recombinant viruses prepared by Dumbell & Bedson (1964) from variola and cowpox and from variola and rabbitpox viruses were tested for the production of ‘d’ substance. These results were compared with the previously recorded effects of these recombinants when inoculated intradermally in rabbits. It is concluded that functional genes determining the production of ‘d’ and of rabbit skin pathogenicity are closely linked on the pox virus genome, but that there is insufficient evidence to say that the two functions are interdependent.

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Hybrids derived from the viruses of alastrim and rabbit pox

Cited by 25 publications

References 10 publications

Genetic Recombination between Vaccinia Virus Strains Distinct in Hemagglutinin Production and Plaquing Efficiency

Genetic Recombination between Vaccinia Virus Strains Distinct in Hemagglutinin Production and Plaquing Efficiency

Hybrids derived from the viruses of variola major and cowpox

A poxvirus antigen associated with pathogenicity for rabbits

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