Hydra protein reduces the toxicity of Ag–PVP nanoparticles in a 3D A549 cell line

Lee, Jin Ah; Yeo, Min‐Kyeong; Kim, Soon-Seok

doi:10.1007/s13273-019-00061-w

Cited by 5 publications

(9 citation statements)

References 46 publications

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“…Lee, et al, identified reduced cytotoxicity of PVP-AgNPs 10-20 nm in combination with a hydra protein in a 2D and 3D A549 model. Cytotoxicity in the form of apoptotic and necrotic cells was observed in the 2D cell culture in minor concentration than in the 3D model; this exhibits that the 3D model is an accurate model to simulate the in vivo conditions since more factors are involved [27].…”

Section: Polyvinylpyrrolidone-agnpsmentioning

confidence: 81%

“…Interestingly, it is possible to identify a coating-dependent gene expression from Table 4. Casp3 is upregulated by uncoated [59] or plant extract-AgNPs [52]; on the contrary, PVP-AgNPs downregulate [41] or do not modify [27] the casp3 expression. The same behavior was found for p53 expression.…”

Section: Mechanisms Of Agnpsmentioning

confidence: 91%

“…The same behavior was found for p53 expression. Wogonin-AgNPs formulation increases its expression [113]; meanwhile, PVP-AgNP produces no change [27] or downregulation [41] in p53 expression.…”

Section: Mechanisms Of Agnpsmentioning

confidence: 94%

“…On the other hand, complex models such as co-cultures and 3D models provide access to apical and basolateral compartments, which can be seeded onto a permeable membrane [22,25]. Hence, the characteristics of physiology and functionality obtain relevance in vitro for further extrapolation to ADME in vivo and barrier integrity studies [22,23,27]. However, the results obtained should be considered carefully to avoid misleading interpretations.…”

Section: Sizementioning

confidence: 99%

“…However, selective cytotoxicity to adenocarcinoma human alveolar basal epithelial cells (A549 cells) compared to their healthy counterpart BEAS-2B cells, is only obtained with the application of ultrasound [26]. Moreover, 10-20 nm polyvinylpyrrolidone-AgNPs (PVP-AgNPs) combined with hydra protein produced a similar result in 3D cultures of A549 exposed to them [27], in which viability was less affected than 2D monocultures of the same cellular line. In contrast, the ex vivo precision-cut lung slices (PCLS) model of C57/Bl6 female rats elucidates a more significant cytotoxic response than their in vitro human counterparts' lung fibroblasts (HLF-1).…”

Section: Sizementioning

confidence: 99%

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Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health

et al. 2022

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Nanomaterials (NMs) solve specific problems with remarkable results in several industrial and scientific areas. Among NMs, silver nanoparticles (AgNPs) have been extensively employed as drug carriers, medical diagnostics, energy harvesting devices, sensors, lubricants, and bioremediation. Notably, they have shown excellent antimicrobial, anticancer, and antiviral properties in the biomedical field. The literature analysis shows a selective cytotoxic effect on cancer cells compared to healthy cells, making its potential application in cancer treatment evident, increasing the need to study the potential risk of their use to environmental and human health. A large battery of toxicity models, both in vitro and in vivo, have been established to predict the harmful effects of incorporating AgNPs in these numerous areas or those produced due to involuntary exposure. However, these models often report contradictory results due to their lack of standardization, generating controversy and slowing the advances in nanotoxicology research, fundamentally by generalizing the biological response produced by the AgNP formulations. This review summarizes the last ten years’ reports concerning AgNPs’ toxicity in cellular respiratory system models (e.g., mono-culture models, co-cultures, 3D cultures, ex vivo and in vivo). In turn, more complex cellular models represent in a better way the physical and chemical barriers of the body; however, results should be used carefully so as not to be misleading. The main objective of this work is to highlight current models with the highest physiological relevance, identifying the opportunity areas of lung nanotoxicology and contributing to the establishment and strengthening of specific regulations regarding health and the environment.

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Section: Polyvinylpyrrolidone-agnpsmentioning

confidence: 81%

Section: Mechanisms Of Agnpsmentioning

confidence: 91%

Section: Mechanisms Of Agnpsmentioning

confidence: 94%

Section: Sizementioning

confidence: 99%

Section: Sizementioning

confidence: 99%

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Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health

et al. 2022

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Binding of silver nanowaste using jellyfish immune reaction extract and an assessment of aquatic toxicity

Kim

Yeo

Lee

et al. 2021

Mol. Cell. Toxicol.

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Physical and biomimetic treatment methods to reduce microplastic waste accumulation

Lee

Shim

Park

et al. 2022

Mol. Cell. Toxicol.

Self Cite

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Background Since the Covid-19 pandemic in 2019, the use of plastics has increased exponentially, so it is imperative to manage and dispose of these plastic wastes safely. Objectives This review focuses on the management strategies governed by the policies of each country to reduce plastic waste through physical collection methods and methods that use eco-imitation technologies. Results Thus far, physical treatment methods have been applied to sewage and drinking water treatment. The abilities of bio-inspired treatment methods are being assessed in terms of capturing microplastics (MPs) and nanoplastics (NPs), extracting substances from marine organisms, reducing toxicity, and developing alternatives to petroleum-based plastics. Conclusions Various post-treatment methods have been proposed to collect and remove MPs and NPs that have reached into aquatic ecosystems and subsequently reduce their toxicity. However, there are limitations that the effectiveness of these methods is hindered by the lack of policies governing the entire process of plastic use before the post-treatment. Purpose of Review We purpose to reduce plastic waste through methods that use eco-imitation technologies. Recent Findings These eco-imitation methods are attracting attention as viable future plastic waste treatment options in line with the goals of sustainable development.

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Hydra protein reduces the toxicity of Ag–PVP nanoparticles in a 3D A549 cell line

Cited by 5 publications

References 46 publications

Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health

Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health

Binding of silver nanowaste using jellyfish immune reaction extract and an assessment of aquatic toxicity

Physical and biomimetic treatment methods to reduce microplastic waste accumulation

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