Hydratation of erythrocyte membranes and their interaction with ultrafine silica

Abstract

The in ter ac tion of ultrafine sil ica (UFS) with mem brane prep a ra tions of do nor eryth ro cytes (ghosts) was in ves ti gated by the method of 1 H NMR spec tros copy. The val ues of their hydratation in con tact with sil ica, interphase en ergy, con cen tra tions of weakly and strongly bound wa ter were mea sured and cal cu lated. Pos si ble mech a nisms of UFS ef fect on cell hemolysis are dis cussed.

Interaction of unmodified and partially silylated nanosilica with red blood cells

“…Typically, at similar temperatures mobile interfacial water is observed (e.g. by 1 H NMR spectroscopy [37]) in hydrated proteins, cells and other bio-objects [18,24]. Decomposition of large water structures in the RBC/A-300 system at C A−300 = 0.01 and 1 wt% is also confirmed by the calculations of the dielectric constant [24] (for unfrozen bound water/RBCs/silica structures) which decreases from 7.8 − 10 (buffered individual RBCs) to 3.7 − 4.4 (RBC/A-300 at C A−300 = 0.01 wt%) and 2.2 − 2.7 (C A−300 = 1 wt%) calculated from the TSDC data at T < 210 K. Typically the smaller the water clusters (water with bio-organics here), the lower the dielectric constant.…”

“…The NMR study of interaction of shadow corpuscles with nanosilica is described in detail elsewhere [37]. …”