Hydrazines as Substrates and Inhibitors of the Archaeal Ammonia Oxidation Pathway

Schatteman, Arne; Wright, C.; Crombie, Andrew T.; Murrell, J. Colin; Lehtovirta-Morley, Laura E.

doi:10.1128/aem.02470-21

Cited by 12 publications

(9 citation statements)

References 44 publications

(72 reference statements)

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“…57 Thus, disruption of the electron transfer chain due to strong AMO inhibition by MPT should also lead to (partial) inhibition of HAO, similar to what has been previously found for the AMO inhibitors DMP and hydrazine. 30,58 Measurement of the Oxygen (O 2 ) Consumption. As the rate of O 2 uptake by AMO is proportional to the oxidation of NH 3 to NH 2 OH, 57 exploration of the kinetics of O 2 consumption in the presence of MPT enables to obtain insight into the mechanism of enzyme inhibition.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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4-Methyl-1-(prop-2-yn-1-yl)-1H-1,2,3-triazole (MPT): A Novel, Highly Efficient Nitrification Inhibitor for Agricultural Applications

Yildirim,

Nathanael,

Frindte

et al. 2024

ACS Agric. Sci. Technol.

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Nitrogen fertilization in agriculture has serious environmental consequences, including production of the greenhouse gas nitrous oxide (N2O), pollution of groundwater with nitrate (NO3 –), and river eutrophication. Nitrogen use efficiency can be increased by amending fertilizers with inhibitors to slow microbial nitrification processes, which transform ammonia to NO3 –. Unfortunately, commercial inhibitors have failed to perform reliably across various agroecosystems for reasons not well understood. Using a combination of bacterial studies and soil incubations, we demonstrate here that 4-methyl-1-(prop-2-yn-1-yl)-1H-1,2,3-triazole (MPT) exhibits superior nitrification inhibitory properties. Unlike the commercial reversible inhibitors, MPT acts as a mechanistic, irreversible inhibitor of the key enzyme ammonia monooxygenase, enabling effective retention of ammonium (NH4 +) and suppression of NO3 – and N2O production over 21 days in several agricultural soils with pH values ranging from 4.7 to 7.5. A bacterial viability stain and a suite of freshwater and terrestrial ecotoxicity tests did not indicate any acute or chronic toxicity. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed an enhanced inhibitory effect of MPT on both ammonia-oxidizing bacteria and archaea. Thus, MPT outperforms currently available nitrification inhibitors and has great potential for broad application in various agricultural settings.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…However, AMO and HAO are not independently operating enzymes but are interconnected through an electron shuttle mechanism . Thus, disruption of the electron transfer chain due to strong AMO inhibition by MPT should also lead to (partial) inhibition of HAO, similar to what has been previously found for the AMO inhibitors DMP and hydrazine. , …”

Section: Resultsmentioning

confidence: 99%

4-Methyl-1-(prop-2-yn-1-yl)-1H-1,2,3-triazole (MPT): A Novel, Highly Efficient Nitrification Inhibitor for Agricultural Applications

Yildirim,

Nathanael,

Frindte

et al. 2024

ACS Agric. Sci. Technol.

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“…AmoC_B is an important subunit of ammonia monooxygenase, which participates in the oxidation of ammonia nitrogen to NH 2 OH [ 50 , 51 ]. The nitrite oxidoreductase encoded by the hao gene is involved in the oxidation of NH 2 OH to nitrite in the nitrogen cycle and serves as a key regulatory factor in the process [ 52 ]. In the present study, the relative abundance of amoC_B and hao genes was higher in the group with adding probiotics compared to other groups.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water

Mang,

Gao,

et al. 2024

Microorganisms

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Recently, probiotics have been widely applied for the in situ remediation of aquatic water. Numerous studies have proved that probiotics can regulate water quality by improving the microbial community. Nitrogen cycling, induced by microorganisms, is a crucial process for maintaining the balance of the aquatic ecosystem. Nevertheless, the underlying mechanisms by which probiotics enhance water quality in aquatic systems remain poorly understood. To explore the water quality indicators and their correlation with nitrogen cycling-related functional genes, metagenomic analysis of element cycling was performed to identify nitrogen cycling-related functional genes in Coilia nasus aquatic water between the control group (C) and the groups supplemented with probiotics in feed (PF) or water (PW). The results showed that adding probiotics to the aquatic water could reduce the concentrations of ammonia nitrogen (NH4+-N), nitrite (NO2−-N), and total nitrogen (TN) in the water. Community structure analysis revealed that the relative abundance of Verrucomicrobiota was increased from 30 d to 120 d (2.61% to 6.35%) in the PW group, while the relative abundance of Cyanobacteria was decreased from 30 d to 120 d (5.66% to 1.77%). We constructed a nitrogen cycling pathway diagram for C. nasus aquaculture ponds. The nitrogen cycle functional analysis showed that adding probiotics to the water could increase the relative abundance of the amoC_B and hao (Nitrification pathways) and the nirS and nosZ (Denitrification pathways). Correlation analysis revealed that NH4+-N was significantly negatively correlated with Limnohabitans, Sediminibacterium, and Algoriphagus, while NO2−-N was significantly negatively correlated with Roseomonas and Rubrivivax. Our study demonstrated that adding probiotics to the water can promote nitrogen element conversion and migration, facilitate nitrogen cycling, benefit ecological environment protection, and remove nitrogen-containing compounds in aquaculture systems by altering the relative abundance of nitrogen cycling-related functional genes and microorganisms.

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“…The relative abundance of active nitrifying families Nitrospiraceae and Nitrosomonadacea was higher in the test reactor than the control for the first 38 days of pharmaceutical exposure (Figure 6), which may explain why nitrogen removal briefly recovered between days zero and 20 (Figure 1). Nitrifier activity may also be responsible for pharmaceutical degradation in the first 12 days of the test: the ammonia monooxygenase enzyme used by both ammonia oxidizing bacteria and archaea (AOA) is known to react non-specifically with aromatic compounds, typically through an oxidation reaction (Keener and Arp, 1994;Schatteman et al, 2022). The primary degradation product of DCF, and all detected GEM degradation products, are formed through oxidation reactions.…”

Section: Links Between Active Bacterial Community Wastewater Treatmen...mentioning

confidence: 99%

Treatment performance and microbial community structure in an aerobic granular sludge sequencing batch reactor amended with diclofenac, erythromycin, and gemfibrozil

Bodle,

Mueller,

Pernat

et al. 2023

Front. Microbiomes

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This study characterizes the effects of three commonly detected pharmaceuticals—diclofenac, erythromycin, and gemfibrozil—on aerobic granular sludge. Approximately 150 µg/L of each pharmaceutical was fed in the influent to a sequencing batch reactor for 80 days, and the performance of the test reactor was compared with that of a control reactor. Wastewater treatment efficacy in the test reactor dropped by approximately 30-40%, and ammonia oxidation was particularly inhibited. The relative abundance of active Rhodocyclaceae, Nitrosomonadaceae, and Nitrospiraceae families declined throughout exposure, likely explaining reductions in wastewater treatment performance. Pharmaceuticals were temporarily removed in the first 12 days of the test via both sorption and degradation; both removal processes declined sharply thereafter. This study demonstrates that aerobic granular sludge may successfully remove pharmaceuticals in the short term, but long-term tests are necessary to confirm if pharmaceutical removal is sustainable.

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Hydrazines as Substrates and Inhibitors of the Archaeal Ammonia Oxidation Pathway

Abstract: Ammonia-oxidizing archaea (AOA) are among the most numerous living organisms on Earth, and they play a pivotal role in the global biogeochemical nitrogen cycle. Despite this, little is known about the physiology and metabolism of AOA.

Cited by 12 publications

References 44 publications

4-Methyl-1-(prop-2-yn-1-yl)-1H-1,2,3-triazole (MPT): A Novel, Highly Efficient Nitrification Inhibitor for Agricultural Applications

4-Methyl-1-(prop-2-yn-1-yl)-1H-1,2,3-triazole (MPT): A Novel, Highly Efficient Nitrification Inhibitor for Agricultural Applications

Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water

Treatment performance and microbial community structure in an aerobic granular sludge sequencing batch reactor amended with diclofenac, erythromycin, and gemfibrozil

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