Hydro‐Sensitive, In Situ Ultrafast Physical Self‐Gelatinizing, and Red Blood Cells Strengthened Hemostatic Adhesive Powder with Antibiosis and Immunoregulation for Wound Repair

Abstract

Immediate and effective hemostatic treatments for complex bleeding wounds are an urgent clinical demand. Hemostatic materials with characteristics of adhesion, sealing, anti‐infection, and concrescence promotion have drawn growing concerns. However, pure natural multifunctional hemostatic materials with in situ ultrafast self‐gelation are rarely reported. In this study, a hydro‐sensitive collagen/tannic acid (ColTA) natural hemostatic powder is developed that can in situ self‐gel to form adhesive by the non‐covalent crosslinking between tannic acid (TA) and collagen (Col) in liquids. The physical interactions endow ColTA adhesive with the characteristics of instantaneous formation and high adhesion at various substrate surfaces. Crucially, ColTA powder adhesive shows an enhanced adhesion performance in the presence of blood due to the electrostatic interactions between ColTA adhesive and red blood cells, conducive to effective in situ sealing and rapid hemostasis. The biocompatible and hemocompatible ColTA adhesive can effectively control bleeding and seal the wounds of the caudal vein, liver, heart, and femoral arteries in rats. Furthermore, the low‐cost and ready‐to‐use ColTA adhesive powder also possesses good antibacterial and inhibiting biofilm formation ability, and can efficiently regulate immune response by the NF‐κB pathway to promote wound repair, making it a highly promising hemostatic material with great potential for biomedical applications.

Durable and Robust Antibacterial Polypropylene Hernia Mesh for Abdominal Wall Defect Repair

“…In the meantime, as compared to the PA/PHMG(1) and PA/PHMG(2) surfaces, an obvious peak at 287.15 eV was observed, which is attributed to the much higher intensity of CN peak detected on the corresponding PA/PHMG(3) surface (Figure h), in accordance with the fact that PHMG possesses massive N species (amino and guanidino groups) and reflecting the successful deposition of PA/PHMG coating on PP meshes. In addition, the π–π* peak at 291.24 eV further confirmed the presence of PA in the PA/PHMG(3) surface …”

“…In addition, the π−π* peak at 291.24 eV further confirmed the presence of PA in the PA/PHMG(3) surface. 33 Moreover, the WCA of pristine PP, PA/PHMG(1), PA/ PHMG(2), and PA/PHMG(3) was tested to investigate the hydrophilic properties of the different sample surfaces (Figure 2i). The pristine PP was inherently hydrophobic and showed a WCA of around 97.17°.…”

Metal–Polyphenol–Collagen Self-Assembled Bioadhesive with Immune Regulation for Infected Wound Healing

“…The antibacterial performances of the ColTA-Zn adhesive were investigated to previous reports . Typically, ColTA-Zn powders (20, 50, and 80 mg) were added to 1 mL of normal saline to prepared material leach liquors.…”

“…In addition, our previous study has demonstrated the biocompatibility of ColTA powder. 13 In this study, a live/dead cell staining assay was used for assessing the cytocompatibility. The results showed that after 48 h of culture with the ColTA-Zn material leach liquor, the proportion of living cells (green) of the ColTA-Zn group was similar to that of the control group (Figure 5a).…”

“…Tannic acid (TA), a natural polyphenol derived from many herbs, can act as a cross-linker to interact with various polymers due to its abundant phenolic hydroxyl group . Based on the polyphenol–protein reaction mechanism, our previous study has developed a self-gelled collagen/tannic acid adhesive powder via the hydrogen bonds between Col-II and TA . Nevertheless, the self-gelled adhesive by hydrogen bonds cross-linking, in comparison with synthetic polymer hydrogels, shows relatively weak cross-linking effect and mechanical properties, thus making it difficult to satisfy the high demands of a promising skin dressing.…”