Hydroalcoholic Extract of Cyperus rotundus Ameliorates H2O2-Induced Human Neuronal Cell Damage via Its Anti-oxidative and Anti-apoptotic Machinery

Kumar, K. Hemanth; Khanum, Farhath

doi:10.1007/s10571-012-9865-8

Cited by 52 publications

(23 citation statements)

References 43 publications

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“…Antioxidants are exogenous or endogenous molecules which act against oxidative stress by neutralizing ROS and other kinds of free radicals, thus exhibiting their therapeutic potential. Hydrogen peroxide-induced cytotoxicity is the general method employed for the measurement of potential neuroprotective antioxidants (Ghaffari et al 2014;Hemanth Kumar and Khanum 2013). The principal objective of this study was to explore the protective effects of bikaverin, a secondary metabolite produced by F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of Bikaverin on H2O2-Induced Oxidative Stress Mediated Neuronal Damage in SH-SY5Y Cell Line

et al. 2014

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The generation of free radicals and oxidative stress has been linked to several neurodegenerative diseases including Parkinson's disease, Alzheimer's disease, Huntington's disease, and Amyotrophic lateral sclerosis. The use of free radical scavenging molecules for the reduction of intracellular reactive oxygen species is one of the strategies used in the clinical management of neurodegeneration. Fungal secondary metabolism is a rich source of novel molecules with potential bioactivity. In the current study, bikaverin was extracted from Fusarium oxysporum f. sp. lycopersici and its structural characterization was carried out. Further, we explored the protective effects of bikaverin on oxidative stress and its anti-apoptotic mechanism to attenuate H 2 O 2 -induced neurotoxicity using human neuroblastoma SH-SY5Y cells. Our results elucidate that pretreatment of neurons with bikaverin attenuates the mitochondrial and plasma membrane damage induced by 100 lM H 2 O 2 to 82 and 26 % as evidenced by MTT and LDH assays. H 2 O 2 induced depletion of antioxidant enzyme status was also replenished by bikaverin which was confirmed by Realtime Quantitative PCR analysis of SOD and CAT genes. Bikaverin pretreatment efficiently potentiated the H 2 O 2 -induced neuronal markers, such as BDNF, TH, and AADC expression, which orchestrate the neuronal damage of the cell. The H 2 O 2 -induced damage to cells, nuclear, and mitochondrial integrity was also restored by bikaverin. Bikaverin could be developed as a preventive agent against neurodegeneration and as an alternative to some of the toxic synthetic antioxidants.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of Bikaverin on H2O2-Induced Oxidative Stress Mediated Neuronal Damage in SH-SY5Y Cell Line

et al. 2014

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“…The data are expressed as percent of inhibition in relation to respective control (without plant extract). Where indicated, solutions of FeSO 4 were made just before use in distilled water.…”

Section: Lipid Peroxidation Assay In Brain Homogenatesmentioning

confidence: 99%

“…Of particular importance, during last years it has been given a special attention to the role of OS in neurodegenerative disorders 3 . Accordingly, as compared to other organs, brain is characterized by its high oxygen consumption rate, high levels of fatty acids, reduced ability of cell regeneration and low levels of antioxidant enzymes 4 . Thus, pharmacological approach to resist OS seems to be a promising therapeutic strategy to counteract neurodegenerative disorders.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of some Medicinal Plant Extracts: Implications for Neuroprotection

Puntel¹,

Tamborena²,

Gularte³

et al. 2015

Pharmacologia

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Background and Objective:This study was conducted in order to evaluate the effect of aqueous and ethanolic extracts of Phyllanthus niruri, Uncaria tomentosa and Mentha pulegium against Lipid Peroxidation (LP). Additionally, it was determined the ability of these plants to scavenge specific free radicals and the interaction with iron ions. Methodology: The LP, scavenging activity, total phenolics and flavonoids were determined by colorimetric methods, whereas HPLC analysis was used to characterize the phytoconstituents. Results: Extracts significantly prevented both basal or induced (by Fe 2+ , malonate, sodium nitroprusside or ferrocyanide) LP. Accordingly, the extracts were better antioxidants at higher used concentrations, the effect of aqueous seems to be higher than ethanolic; the effect of plants were found to be P. niruri>M. pulegium>U. tomentosa; extracts were better antioxidants when used in rat brain homogenates, as compared to yolk phospholipids. Extracts also presented significant scavenging activity, prevented deoxyribose degradation and chelate Fe 2+. The HPLC analysis revealed that the extracts have different types and quantities of phytoconstituents. Conclusion: Altogether, presented results support the idea that antioxidant activities of extracts are related to the ability to scavenge specific free radicals and/or interaction with redox chemistry of iron ions. Thus, the broad range of antioxidant activity of these plants indicates the use with potential application to reduce LP associated to neurodegenerative disorders.

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“…This herb is also widely used as an analgesic, nerve tonic, nootropic, sedative, anti-spasmodic, anti-malarial, antidiarrheal and a reliever of stomach disorders (13)(14)(15)(16)(17). Previous pharmacological investigations indicated that the extracts and/ or components of C. rotundus rhizomes have marked hypotensive, anti-mutagenic, anti-obese, neuroprotective, antioxidant, anti-inflammatory and anti-pyretic effects (18)(19)(20)(21)(22)(23)(24)(25)(26). In addition, this herb is often prescribed in combination with other herbs to treat stomach ache, inflammation and peptic ulcer disease (27,28).…”

Section: Induction Of Apoptosis In Mda-mb-231 Human Breast Carcinoma mentioning

confidence: 99%

Induction of apoptosis in MDA-MB-231 human breast carcinoma cells with an ethanol extract of Cyperus rotundus L. by activating caspases

et al. 2014

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Abstract. Cyperus rotundus L. belongs to the Cyperaceae family and is a well documented traditional medicinal herb. Its rhizome has been reported to possess wide spectrum pharmacological activities including anti-inflammatory and antioxidant activity. However, the cellular and molecular mechanisms of the anticancer activity have not been elucidated. In the present study, we investigated the pro-apoptotic effects of C. rotundu rhizomes in a human breast carcinoma MDA-MB-231 cell model. Treatment of MDA-MB-231 cells with an ethanol extract of C. rotundu rhizomes (EECR) and a methanol extract of C. rotundu rhizomes (MECR), but not a water extract of C. rotundu rhizomes, resulted in potent antiproliferative activity. The activity of the EECR was higher than that of the MECR and was associated with the induction of apoptosis. The induction of apoptosis by the EECR was associated with upregulation of death receptor 4 (DR4), DR5 and pro-apoptotic Bax, as well as downregulation of anti-apoptotic survivin and Bcl-2. EECR treatment also downregulated Bid expression and activated caspase-8 and -9, the respective initiator caspases of the extrinsic and intrinsic apoptotic pathways. The increase in mitochondrial membrane depolarization was correlated with activation of effector caspase-3 and cleavage of poly(ADP-ribose) polymerase, a vital substrate of activated caspase-3. Blockage of caspase activation by pretreatment with a pan-caspase inhibitor consistently inhibited apoptosis and abrogated growth inhibition in EECR-treated MDA-MB-231 cells. Although reactive oxygen species (ROS) increased following treatment with the EECR, inhibiting ROS with a ROS scavenger did not attenuate EECR-induced apoptosis. Furthermore, inhibitors of phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK) signaling pathways failed to reverse EECR-induced apoptosis and growth inhibition. These results suggest that the pro-apoptotic activity of the EECR may be regulated by a caspase-dependent cascade through activation of both intrinsic and extrinsic signaling pathways that is not associated with ROS generation or the PI3K/Akt and MAPK pathways.

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Hydroalcoholic Extract of Cyperus rotundus Ameliorates H2O2-Induced Human Neuronal Cell Damage via Its Anti-oxidative and Anti-apoptotic Machinery

Cited by 52 publications

References 43 publications

Neuroprotective Effects of Bikaverin on H2O2-Induced Oxidative Stress Mediated Neuronal Damage in SH-SY5Y Cell Line

Neuroprotective Effects of Bikaverin on H2O2-Induced Oxidative Stress Mediated Neuronal Damage in SH-SY5Y Cell Line

Antioxidant Activity of some Medicinal Plant Extracts: Implications for Neuroprotection

Induction of apoptosis in MDA-MB-231 human breast carcinoma cells with an ethanol extract of Cyperus rotundus L. by activating caspases

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