Stable carbon isotopic compositions were determined for diploptene in the sediment core KH-79-3, L 3 (core length: 235 cm) taken from the Oki Ridge in the southern part of the Japan Sea. Two major groups of diploptene with different 813C values were recognized. The first group appears in the 0-130 cm section (present to 10 kyr B.P.) of the core, the 813C values being -25.0 ± 0.7%o on average. Diploptene in this section should have originated from cyanobacteria. The second group which is strongly depleted in 13C ( 53.1 ± 5.3%o on average), is observed in the 145-204 cm section (12-21 kyr B.P.). Diploptene in this section might have originated from methanotrophic bacteria. This indicates that an anoxic bottom water condition was developed during 12-21 kyr B.P. (the last glacial period), which was perhaps caused by the development of seawater stratification in the Japan Sea.