Columnar sediment samples were collected from five representative estuaries of Dianchi Lake, China. And the vertical distribution of each fraction of nitrogen (IEF-N, CF-N, IMOF-N, OSF-N) were tested. The results showed that the TN content in sediments from areas A, B, C, D and E gradually decreased with depth between 0 and 15 cm, then sharply decreased with depth between 15 and 30 cm and stabilized at depth below 30 cm, indicating the exogenous input of N in these areas has not been controlled effectively. The proportion of TN occupied by various N fractions in the sediments ranked as follows: OSF-N > IMOF > CF-N > IEF-N. Correlation analysis results showed both IEF-N and IMOF-N were significantly correlated with the content of TFe 2 O 3 + MnO + Al 2 O 3 in deeper sediments, while no correlation in superficial sediments. The areas A and B have extremely high release risks for N in superficial sediments. However, the N in the sediments of areas C, D and E were in relative equilibrium with the overlying water, indicating release potential risk was relatively low.