Hydrocarbon Utilizing and Metal Tolerant Bacteria Simultaneously Degrade Hydrocarbons and Detoxify Metals in Petroleum Contaminated Soil

Wani, Parvaze Ahmad; Ebudola, Adebimpe Nusirat; Olusebi, Yusuff Kareem; Rafi, Nusrat; O, Sanusi Jadesola Fawzhia; Oluwaseun, Oyelami Isaac

doi:10.1080/01490451.2023.2176573

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…In most cases, Lysinibacillus fusiformis has been isolated from soil, water, and plants. The research focus has been limited to the production of biosurfactants [55] and exopolysaccharides [48], degradation of crude oil [56], soil pollution remediation [57], inhibition of fungi [53], algae-lytic activity, and productivity of microalgae [58]. However, there are few reports on the control of nematode diseases.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol Potential of Bacteria Isolated from Vermicompost against Meloidogyne incognita on Tomato and Cucumber Crops

Liang,

Yang,

Dong

et al. 2024

Horticulturae

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Root-knot nematodes are the major diseases in protected cultivation around the world. Bio-organic fertilizer has become a research hotspot, with a variety of microorganisms that control various vegetable soil-borne diseases. This study screened nematocidal microorganisms from fresh vermicompost, explored the inhibitory substances produced by biocontrol agents, and evaluated their potential biocontrol ability in the pot and field under greenhouse conditions. The highly effective antagonistic microbes of Meloidogyne incognita (M. incognita) were screened. Strains YL1 and YL31 were identified as Peribacillus frigoritolerans, and strain YL6 was identified as Lysinibacillus fusiformis. The three strains all produced chitinase and protease, which prevented the normal development of eggs and the second-stage juveniles (J2) by destroying their appearance. The three strains all improved potassium-dissolving ability, and the strains YL1 and YL6 also enhanced phosphorus-dissolving ability. Pot experiments showed that tomato root knots were reduced, and plant growth improved. Field tests showed that the root-knot index and nematode population were reduced significantly, and cucumber growth and yield were enhanced. Strain YL1 had the best control effect with 70.6%, and the yield increased by 14.9% compared with the control. Overall, this study showed the ability of antagonistic bacteria YL1, YL6, and YL31 to control root-knot nematodes, and these antagonistic bacteria could be developed as biocontrol agents for sustainable agriculture.

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Section: Discussionmentioning

confidence: 99%

Biocontrol Potential of Bacteria Isolated from Vermicompost against Meloidogyne incognita on Tomato and Cucumber Crops

Liang,

Yang,

Dong

et al. 2024

Horticulturae

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“…therefore could find applicability as an ideal model organism for manganese removal from ferro-manganese contaminated sites in estuaries and hypersaline environments. Wani et al (2023) conducted in vitro studies and documented the use of heterotrophic bacteria as bio-inoculants beneficial in the removal of metal pollutants.…”

Section: Discussionmentioning

confidence: 99%

A halophilic Chromohalobacter species from estuarine coastal waters as a detoxifier of manganese, as well as a novel bio-catalyst for synthesis of n-butyl acetate

Pereira¹,

Kerkar

Dias³

et al. 2023

Front. Microbiol.

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Anthropogenic pollution due to ferro-manganese ore transport by barges through the Mandovi estuary in Goa, India is a major environmental concern. In this study a manganese (Mn) tolerant, moderately halophilic Chromohalobacter sp. belonging to the family Halomonadaceae was isolated from the sediments of a solar saltern adjacent to this Mandovi estuary. Using techniques of Atomic absorption spectroscopy, Scanning electron microscopy-Energy dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy and Atomic Force Microscopy, the Chromohalobacter sp. was explored for its ability to tolerate and immobilize Mn in amended and unamended media with 20% natural salt concentration (w/v). In aqueous media supplemented with 0.1 mM Mn, the Chromohalobacter sp. was capable of sequestering up to 76% Mn with an average immobilization rate of 8 mg Mn /g /day. Growth rate kinetic analysis using Gompertz mathematical functions was found to model the experimental data well. The model inferred that the maximum growth rate of Chromohalobacter sp. was at 10% natural salt concentration (w/v). The Chromohalobacter sp. was further found to be multimetal tolerant showing high tolerance to Iron (Fe), Nickel (Ni) and Cobalt (Co), (each at 4 mM), and tolerated Manganese (Mn) up to 6 mM. Morphologically, the Chromohalobacter sp. was a non-spore forming, Gram negative motile rod (0.726 μ× 1.33 μ). The adaptative mechanism of Chromohalobacter sp. to elevated Mn concentrations (1 mM) resulted in the reduction of its cell size to 0.339 μ× 0.997 μ and the synthesis of an extracellular slime, immobilizing Mn from the liquid phase forming Manganese oxide, as confirmed by Scanning Electron Microscopy. The expression of Mnx genes for manganese oxidation further substantiated the finding. This bacterial synthesized manganese oxide also displayed catalytic activity (∼50% conversion) for the esterification of butan-1-ol with CH3COOH to yield n-butyl acetate. This Chromohalobacter sp. being indigenous to marine salterns, has adapted to high concentrations of heavy metals and high salinities and can withstand this extremely stressed environment, and thus holds a tremendous potential as an environmentally friendly “green bioremediator” of Mn from euryhaline environments. The study also adds to the limited knowledge about metal-microbe interactions in extreme environments. Further, since Chromohalobacter sp. exhibits commendable catalytic activity for the synthesis of n-butyl acetate, it would have several potential industrial applications.

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“…Bioremediation is one mechanism used in many cases for cleaning polluted environments. Hydrocarbon or oil biodegradation and metal remediation is carried out efficiently by natural indigenous microbes and is an enzymatic process [50].…”

Section: Discussionmentioning

confidence: 99%

Remediation of hydrocarbons and metals by hydrocarbon utilizing Pseudomonas taiwanensis YSA‐17 isolated from soil contaminated with petroleum

Wani,

Olusebi,

Rilwan

2023

J Basic Microbiol

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Careless handling of petroleum in petrochemical industries releases toxic hydrocarbons and metals to soil and water. The aim of the present study was to isolate hydrocarbon‐utilizing and metal‐tolerant bacteria. Hydrocarbon‐utilizing bacteria from petroleum‐contaminated soils were isolated on the Bushnell Hass medium. Hydrocarbon degradation by Pseudomonas taiwanensis strain YSA‐17 was observed by gas chromatography–mass spectrometry. Bioaccumulation of metals by strain YSA‐17 was assessed in nutrient broth. Among different strains, YSA‐17 showed the highest potential for hydrocarbon utilization. After 20 days of incubation, YSA‐17 completely degraded one compound and during its degradation, there was the formation of 13 new compounds which were absent in uninoculated control. Results of scanning electron microscope and Fourier transmission infrared (FTIR) indicated degradation of hydrocarbons. FTIR showed the formation of new functional groups in YSA‐17 inoculated medium. Expression of the total quantity of hydrocarbon‐degrading gene (AlkB and NehAc) in petroleum‐amended nutrient broth inoculated with strain YSA‐17 enhanced significantly during 20 days of incubation compared to control. YSA‐17 also significantly removed metals. This study concluded that bioinoculant can be utilized for the bioremediation of pollutants cocontaminated with hydrocarbons and metals. Petroleum‐contaminated soil will be remediated for pollutants.

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Hydrocarbon Utilizing and Metal Tolerant Bacteria Simultaneously Degrade Hydrocarbons and Detoxify Metals in Petroleum Contaminated Soil

Cited by 8 publications

References 27 publications

Biocontrol Potential of Bacteria Isolated from Vermicompost against Meloidogyne incognita on Tomato and Cucumber Crops

Biocontrol Potential of Bacteria Isolated from Vermicompost against Meloidogyne incognita on Tomato and Cucumber Crops

A halophilic Chromohalobacter species from estuarine coastal waters as a detoxifier of manganese, as well as a novel bio-catalyst for synthesis of n-butyl acetate

Remediation of hydrocarbons and metals by hydrocarbon utilizing Pseudomonas taiwanensis YSA‐17 isolated from soil contaminated with petroleum

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