Hydrocarbon-utilizing bacteria of natural crude oil seepages, Digboi oilfield, Northeastern region of India

Bhagobaty, Ranjan Kumar

doi:10.1007/s43217-020-00013-8

Cited by 11 publications

(8 citation statements)

References 37 publications

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“…Some of the bacteria excrete biosurfactants that emulsify hydrocarbons, use enzymes reacting with hydrocarbons, or biopolymers that convert hydrocarbons into polar compounds that have better solubility in water. Only a few bacteria can utilize hydrocarbons directly [ 65 , 66 , 67 , 68 ]. To overcome the problem of hydrophobicity in high-weight PH fractions, which limits the transfer to microbial cells, cultivation of surfactant-producing bacteria or addition of a biosurfactant to the contaminated substrate should be added [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Zając

Fabiańska

Jedrszczyk

et al. 2022

IJERPH

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To improve the environmental sustainability of cleanup activities of contaminated sites there is a need to develop technologies that minimize soil and habitat disturbances. Cleanup technologies, such as bioremediation, are based on biological products and processes, and they are important for the future of our planet. We studied the potential of γ-poly glutamic acid (PGA) as a natural component of biofilm produced by Bacillus sp. to be used for the decomposition of petroleum products, such as heavy naphtha (N), lubricating oil (O), and grease (G). The study aimed to assess the impact of the use of different concentrations of PGA on the degradation process of various fractions of petroleum hydrocarbons (PH) and its effect on bacterial population growth in harsh conditions of PH contamination. In laboratory conditions, four treatments of PGA with each of the petroleum products (N, O, and G) were tested: PGA0 (reference), PGA1 (1% PGA), PGA1B (1% PGA with Bacillus licheniformis), and PGA10 (10% PGA). After 7, 28, 56, and 112 days of the experiment, the percentage yield extraction, hydrocarbon mass loss, geochemical ratios, pH, electrical conductivity, and microorganisms survival were determined. We observed an increase in PH removal, reflected as a higher amount of extraction yield (growing with time and reaching about 11% in G) and loss of hydrocarbon mass (about 4% in O and G) in all treatments of the PGA compared to the reference. The positive degradation impact was intensive until around day 60. The PH removal stimulation by PGA was also reflected by changes in the values of geochemical ratios, which indicated that the highest rate of degradation was at the initial stage of the process. In general, for the stimulation of PH removal, using a lower (1%) concentration of PGA resulted in better performance than a higher concentration (10%). The PH removal facilitated by PGA is related to the anionic homopoliamid structure of the molecule and its action as a surfactant, which leads to the formation of micelles and the gradual release of PH absorbed in the zeolite carrier. Moreover, the protective properties of PGA against the extinction of bacteria under high concentrations of PH were identified. Generally, the γ-PGA biopolymer helps to degrade the hydrocarbon pollutants and stabilize the environment suitable for microbial degraders development.

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Section: Discussionmentioning

confidence: 99%

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Zając

Fabiańska

Jedrszczyk

et al. 2022

IJERPH

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“…Considering all the criteria employed in evaluating the ef f iciencies of the isolates, Providencia vermicola W8 isolated f rom water source perf ormed the lowest. Few scientists have reported the crude oil degradation potential of this organism (Bhagobaty, 2020;Chikere et al, 2017;Uba et al, 2018). Contrary to the f indings of this present study, Chikere et al (2017) worked with isolates f rom crude oil contaminated soil in Gokana, Rivers State, Nigeria and reported higher degradation potential by Providencia sp than Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Crude oil hydrocarbon degradation efficiency of indigenous bacterial strains isolated from contaminated sites in Nigeria

Nnabuife¹,

Ogbonna²,

Anyanwu³

2022

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The crude oil degradation potential of bacterial isolates from three contaminated sites in Nigeria were investigated. Seven bacterial isolates namely Pseudomonas aeruginosa strain W15, Pseudomonas aeruginosa strain N3R, Serratia marcescens strain N4, Providencia vermicola strain W8, Serratia marcescens strain W13, Pseudomonas aeruginosa strain W11 and Pseudomonas protegens strain P7 were isolated and identified using molecular methods. Isolates N4, N3 and W13 showed higher % total petroleum hydrocarbon (TPH) degradation of 79.26%, 78.96% and 78.69% respectively than W15, P7, W8 and W11 with % TPH degradation of 68.96%, 62.14%, 59.75% and 59.00% respectively. W13 showed the fastest degradation rate with 78.72% within the first 14 days of incubation; however, after the 14th day, there was no progressive change in % TPH. W11 showed degradation of wider range of hydrocarbon components originally in the crude oil as well as the complete degradation of most intermediates formed. The isolates showed good degradation potentials for bioremediation applications.

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“…Observation of heterotrophic bacteria in aerobic condition was conducted in 5 cm-deep sediments because the oxygen level in the sediments at this level is higher than that of the sediments in low depths, leading to high microbial diversity (Dias et al, 2009;Somerfield et al, 1998). A total of 233 bacterial isolates were isolated and identified using biochemical approaches and ABIS online tools (Bhagobaty, 2020;Kayode et al, 2020;Dumitru et al, 2021;Ndukwu et al, 2021;Vansia et al, 2021). The results showed that the bacteria belonged to 29 genera and 16 families.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Community of Klong Tub Mangrove Forest in Chonburi Province, Thailand

Ganjanasiripong

Neesanant

Taechowisan

et al. 2022

Environ. Nat. Resour. J.

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Mangrove forests are located in the transition zone of terrestrial and river/marine ecosystems, making these forests a unique environment harbouring diverse microbes. This study investigated the bacterial community of Klong Tub Mangrove Forest in Chonburi Province, Thailand. The distinct feature of this forest is its nearby location to a narrowleaf cattail wetland. Assessment of the abiotic parameters of the sediments from site#1 nearby the narrowleaf cattail wetland and site#2 in the mangrove forest revealed differences in pH and salinity values between these two sites. Biochemical identification of bacterial isolates (n=233) indicated that these species belonged to 16 families and 29 genera as follows: Moraxellaceae (17.60%) > Vibrionaceae (16.31%) > Paenibacillaceae (15.88%) > Staphylococcaceae and Bacillaceae (9.87% each) > Aeromonadaceae and Pseudomonadaceae (8.58% each) > Enterobacteriaceae (4.29%) > Lactobacillaceae (2.58%) > Moraxellaceae (2.15%) > Comamonadaceae (1.72%) > Alcaligenaceae (0.86%) > Morganellaceae, Burkholderiaceae, Pasteurellaceae and Streptococcaceae (0.43% each). Among the genera, 12 were commonly isolated from both sites. Bacterial strains from 7 and 10 genera were detected only in site#1 and site#2, respectively. Analysis of the partial 16s rRNA gene sequence of four filamentous gram-positive isolates showed their high sequence similarity to three genera, including three novel species, Streptomyces sp. NA03103, Micromonospora fluminis sp. nov. and Bacillus velezensis sp. nov. In conclusion, the Klong Tub Mangrove Forest possesses high microbial diversity, and the bacterial taxon in the sediments differ between the narrowleaf cattail wetland and mangrove forest. Several bacterial isolates from the forest show a high biotechnological potential.

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Hydrocarbon-utilizing bacteria of natural crude oil seepages, Digboi oilfield, Northeastern region of India

Cited by 11 publications

References 37 publications

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Crude oil hydrocarbon degradation efficiency of indigenous bacterial strains isolated from contaminated sites in Nigeria

Bacterial Community of Klong Tub Mangrove Forest in Chonburi Province, Thailand

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