Hydrocarbonoclastic Bacteria From Jakarta Bay and Seribu Islands

Abstract

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It’s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.

Biodegradation of polycyclic aromatic hydrocarbons by a bacterial consortium enriched from mangrove sediments

“…In addition, Darmayati et al . [ 17 ] isolated Pseudidomarina sp. from oil polluted marine sediments but no PAH degrading abilities were reported previously.…”

Insights into Bacterial Community Involved in Bioremediation of Aged Oil-Contaminated Soil in Arid Environment

“… 69 Moreover, this genus was identified among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands that are known as the most polluted marine environment in Indonesia. 70 In addition, a recent study showed the increase of Alcanivorax genus during the remediation of crude oil-polluted soil by bacterial rhizosphere community. 71 Moreover, Alcanivorax borkumensis which is a marine alkane-degrading bacterium harbors enzymes involved in alkane oxidation (cytochromes CYP153 and AlkB homologs which are well-characterized particulate membrane-bound hydroxylase).…”

“… 69 Furthermore, this genus was isolated from oil polluted marine sediments in Indonesia. 70 Moreover, indigenous Pseudidiomarina from mangrove surface sediments of Nay band Bay in Iran showed high potential to degrade fluorene and phenanthrene. 76 In our study, Pseudidiomarina genus showed abundance at T9 simultaneously with an enduring high level of phenanthrene.…”

“…69 Moreover, this genus was identified among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands that are known as the most polluted marine environment in Indonesia. 70 In addition, a recent study showed the increase of Alcanivorax genus during the remediation of crude oil-polluted soil by bacterial rhizosphere community. Control: clean soil with compost; T0: zero day after treatment; T3: 3 months after treatment; T6: 6 months after treatment; T9: 9 months after treatment.…”

Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean

“…Biomolecular approaches have been used for the isolation and identification of oil-degrading bacteria in Indonesia since 2005. Darmayati et al. (2008) isolated 131 different hydrocarbonoclastic bacterial strains from Jakarta Bay and Seribu Island.…”