Aim of this study was to analyze the geochemical mechanisms that influence the composition of groundwater and determine the suitableness of groundwater quality for farming and domestic uses in the agricultural area of Mathura, India. This study also emphasized the significance of using a comprehensive approach which includes water pollution evaluating indexes and multivariate statistical techniques to investigation of hydrochemical analysis of groundwater quality and identification of contamination sources. Water samples were collected from different sampling sites and analyzed. 81.25% and 62.50% of the sampling sites of groundwater above the maximum allowable levels for the concentration of fluoride and nitrate as per BIS, 2012. Cluster analysis categorized sixteen sampling locations into three clusters based (High, moderately and less polluted) on observed data. Piper diagram concluded that the calcium, magnesium, sodium, potassium, are predominate ions showing its unsuitability for irrigation. According to the Wilcox diagram, US salinity laboratory diagram, Kelly index, Magnesium adsorption ratio, Permeability index, 6.25%, 31.25%, 25%, 93.75%, 18.75% respectively, the study area is classified as an unsuitable for irrigation. The present investigation provided useful information of suitable water management resource for healthy crop growth, human health, water resources management, and ultimately the sustainable development of the environment.