Hydrodynamic and Quasi‐elastic Light Scattering Studies on the 12s Globulin From Rapeseed*

Abstract

The 12s globulin, one of the major storage proteins of rapeseeds, has the following physico-chemical constants, as determined by ultracentrifugation, quasi-elastic light scattering measurements and gel chromatography: sedimentation coefficient s:scattering) D&,, = 3.8 X 1 0-7 cm2 s-l; Stokes radius (by quasi-elastic light scattering) R, = 5.7nm and (by gel chromatography) R, = 5.5 nm; partial specific volume (calculated from the amino acid composition) V = 0.729 mlg-'; molecular weight M,,D = 300,000 daltons, Ms,Rs = 294,000 daltons ( R , from the gel chromatography); frictional ratio f J f o = 1.28.

Plant protein interactions studied using AFM force spectroscopy: nanomechanical and adhesion properties

“…The cruciferin is characterized by a low content of helix and a relatively high content of β-sheet conformation. [11][12][13] The ratio of acid to basic amino acid residues is 1.0, the iep is 7.2, 14 and the radius of gyration of the cruciferin, determined by X-ray scattering, is 4.1 nm. 15 Other physicochemical properties for these plant proteins such as aggregation morphology, adhesion and nanomechanical properties have not been yet studied in depth.…”

Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules

“…The dierent conformation must be re¯ected in the universal quantities, whose numerical values have been the subject of successive revisions as the hydrodynamic theory has been improved. Good estimates of F 0 and P 0 have been obtained by Monte Carlo simulation: F 0 = 2.53´10 23 and P 0 = 6.0 for theta conditions [28,29], and F 0 = 1.9´10 23 and P 0 = 5.3 in good solvent conditions [30]. These results can be recast in the form of ratios of radii, for which the values are given in Table 5.…”

“…independent quantities suers some inconveniences. Two of them, unimportant but somehow cumbersome, are related to the diversity not only in the symbols employed to represent them, but mainly in the disparity of their numerical values and the order of magnitude for typical cases; thus, b takes the values of 2.112´10 6 and about 2.3´10 6 for a sphere and a random coil, respectively, while the values for these two structures in the case of the F 0 are 9.23´10 23 and 2.6´10 23 .…”