Hydrodynamic Chromatography in Narrow and Wide-Bores; Whether Radial Diffusion Is Essential or Not

Okada, Tetsuo

doi:10.1080/10826076.2010.484351

Cited by 8 publications

(10 citation statements)

References 25 publications

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“…For an open cylindrical capillary, this dependence can be made explicit analytically in the form where V is the average particle velocity, U is the average velocity of the eluent, and a = d p /(2 R c ) is the ratio of the particle diameter, d p , to the capillary diameter 2 R c , R c being the radius of the capillary. A qualitatively similar dependence is observed in rectangular channels, which may prove more suitable for being integrated in μ-TAS devices. , Laminar flows in open channels have also been used to resolve mixtures of small molecules, either exploiting significant differences in the bare diffusion coefficient of the species or else by entraining molecular aggregates in the flow which possess different affinity toward the molecular species. , Clearly, similar to the case of SEC-based separations, convection-amplified dispersion effects are also present in HDC, yet they are generally less severe and can be reduced by wall patterning strategies. , More importantly, when open channel geometries are being used, the axial dispersion coefficients can be estimated through affordable analytical/numerical approaches, , thus allowing to predict the separation performance with accuracy. In what follows, we refer to HCD separations exploiting open channel geometries as MHDC (microcapillary hydrodynamic chromatography).…”

Section: Introductionmentioning

confidence: 82%

“…16,17 Laminar flows in open channels have also been used to resolve mixtures of small molecules, either exploiting significant differences in the bare diffusion coefficient of the species or else by entraining molecular aggregates in the flow which possess different affinity toward the molecular species. 18,19 Clearly, similar to the case of SEC-based separations, convectionamplified dispersion effects are also present in HDC, yet they are generally less severe and can be reduced by wall patterning strategies. 20,21 More importantly, when open channel geometries are being used, the axial dispersion coefficients can be estimated through affordable analytical/numerical approaches, 22,23 thus allowing to predict the separation performance with accuracy.…”

Section: ■ Introductionmentioning

confidence: 99%

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Brownian Sieving Effect for Boosting the Performance of Microcapillary Hydrodynamic Chromatography. Proof of Concept

et al. 2021

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Microcapillary hydrodynamic chromatography (MHDC) is a well-established technique for the size-based separation of suspensions and colloids, where the characteristic size of the dispersed phase ranges from tens of nanometers to micrometers. It is based on hindrance effects which prevent relatively large particles from experiencing the low velocity region near the walls of a pressure-driven laminar flow through an empty microchannel. An improved device design is here proposed, where the relative extent of the low velocity region is made tunable by exploiting a two-channel annular geometry. The geometry is designed so that the core and the annular channel are characterized by different average flow velocities when subject to one and the same pressure drop. The channels communicate through openings of assigned cut-off length, say A . As they move downstream the channel, particles of size bigger than A are confined to the core region, whereas smaller particles can diffuse through the openings and spread throughout the entire cross section, therein attaining a spatially uniform distribution. By using a classical excluded-volume approach for modeling particle transport, we perform Lagrangian-stochastic simulations of particle dynamics and compare the separation performance of the two-channel and the standard (single-channel) MHDC. Results suggest that a quantitative (up to thirtyfold) performance enhancement can be obtained at operating conditions and values of the transport parameters commonly encountered in practical implementations of MHDC. The separation principle can readily be extended to a multistage geometry when the efficient fractionation of an arbitrary size distribution of the suspension is sought.

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Section: Introductionmentioning

confidence: 82%

Section: ■ Introductionmentioning

confidence: 99%

Brownian Sieving Effect for Boosting the Performance of Microcapillary Hydrodynamic Chromatography. Proof of Concept

et al. 2021

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“…Size-selective separation of particles is an important task in various fields, including micro/nano-science/technology, life science, environmental chemistry, and industrial applications. Particle separation has been conducted using field flow fractionation (FFF), hydrodynamic chromatography, − electrophoresis, − dielectrophoresis, ,, and so forth. In these separation methods, physical fields are utilized to induce different distributions of particles according to their physical properties, such as sizes and charges.…”

Section: Introductionmentioning

confidence: 99%

Size-Tunable Micro-/Nanofluidic Channels Fabricated by Freezing Aqueous Sucrose

et al. 2019

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Upon freezing aqueous sucrose at temperatures higher than the eutectic point (−14 °C in this case), two phases, that is, ice and freeze concentrated solution (FCS), are spontaneously separated. FCS forms through-pore fluidic channels when thin ice septum is prepared from aqueous sucrose. Total FCS volume depends on temperature but is independent of the initial sucrose concentration. This allows us to control the size of the FCS channels simply by changing the initial sucrose concentration as long as temperature is kept constant. In this paper, we show that the size of the channel, which has a layered structure, can be controlled in a range from 50 nm to 3 μm. Thus, the FCS channel is suitable for size-sorting of micro- and nanoparticles. We discuss the size-sorting efficiency of the channel and demonstrate the separation of particles with different sizes.

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“…ACTIS is based on very fast (typically achieved in less than 1 min) transient incomplete separation (TIS) of the complex from the small molecule. TIS of two species always occurs in a pressure-driven laminar flow inside a capillary if their diffusion coefficients differ. − The concept of ACTIS is reiterated in Figure . An equilibrium mixture (EM) of the target and ligand is prepared in an incubation buffer outside of the capillary.…”

mentioning

confidence: 99%

“…There is a universally accepted view that TIS is only applicable to species greatly different in size. Therefore, all examples of TIS in the literature relate to species, which differ in the diffusion coefficient by a factor of 10 or more. − Such a predisposition made previous ACTIS efforts to focus on protein–small-molecule complexes (diffusion coefficients of a small molecule and a protein–small-molecule complex differ by approximately a factor of 10). The goal of this work was to understand if the TIS of protein–large ligand complex from the unbound ligand could be detected and used for K d determination for such complexes.…”

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confidence: 99%

Transient Incomplete Separation of Species with Close Diffusivity to Study the Stability of Affinity Complexes

Wang

Rukundo

et al. 2022

Anal. Chem.

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Large molecules can be generically separated from small ones, though partially and temporarily, in a pressure-driven flow inside a capillary. This transient incomplete separation has been only applied to species with diffusion coefficients different by at least an order of magnitude. Here, we demonstrate, for the first time, the analytical utility of transient incomplete separation for species with close diffusion coefficients. First, we prove in silico that even a small difference in diffusivity can lead to detectable transient incomplete separation of species. Second, we use computer simulation to prove that such a separation can be used for the reliable determination of equilibrium dissociation constant (K d ) of complexes composed of similar-sized molecules. Finally, we demonstrate experimentally the use of this separation for the accurate determination of K d value for a protein−aptamer complex. We conclude that "accurate constant via transient incomplete separation" (ACTIS) can serve as a reference method for affinity characterization of protein−aptamer binding in solution.

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Hydrodynamic Chromatography in Narrow and Wide-Bores; Whether Radial Diffusion Is Essential or Not

Cited by 8 publications

References 25 publications

Brownian Sieving Effect for Boosting the Performance of Microcapillary Hydrodynamic Chromatography. Proof of Concept

Brownian Sieving Effect for Boosting the Performance of Microcapillary Hydrodynamic Chromatography. Proof of Concept

Size-Tunable Micro-/Nanofluidic Channels Fabricated by Freezing Aqueous Sucrose

Transient Incomplete Separation of Species with Close Diffusivity to Study the Stability of Affinity Complexes

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