Hydrodynamic dissection of<i>Stentor coeruleus</i>in a microfluidic cross junction

Paul, Rajorshi; Zhang, Kevin S.; Jalil, Myra Kurosu; Castaño, Nicolas; Kim, Sun-Gu; Tang, Susan

doi:10.1039/d2lc00527a

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Image-based Methods1

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2023

2024

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Cited by 4 publications

(1 citation statement)

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“…The deformability is measured by imaging single-cell geometric changes under the shear stress [93][94][95] or extensional forces. [96][97][98] The accu-racy of cell classification can be improved by combining mechanical typing and deformation dynamics with cell morphology. For example, Otto et al 22 reported a real-time cell deformability cytometry platform (RT-DC) that uses highspeed imaging to measure cell deformability under shear force (Fig.…”

Section: Image-based Methodsmentioning

confidence: 99%

Recent advances in deformation-assisted microfluidic cell sorting technologies

Sun,

Huang,

Chen

et al. 2023

Analyst

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Section: Image-based Methodsmentioning

confidence: 99%

Recent advances in deformation-assisted microfluidic cell sorting technologies

Sun,

Huang,

Chen

et al. 2023

Analyst

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Vortex-induced particle capture in a micro cross-shaped channel

Zhang,

Shi,

Yao

et al. 2024

Separation and Purification Technology

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Adjustable behaviors and dynamic mechanisms of droplets in the cross junction

Liu,

Wang,

Pang

et al. 2023

Physics of Fluids

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Dynamic behaviors of droplets transporting through the cross junction are experimentally and theoretically studied. The influences of initial droplet length, total flux, and flow rate combination of different inlets are systematically analyzed. Under the influence of the opposite flow, more types of flow patterns and different thresholds of two behaviors (breakup and obstruction) are obtained after a comparison with other references. Deformation characteristics in different patterns and stages are discussed in detail regarding the time-dependent lengths and their evolution rates. An empirical relation is proposed to estimate the time difference representing the unsynchronized necking, and the estimations agree well with the experimental results in all cases. Theoretical analysis of the rapid pinch-off during the breakup process proves the important role of the curvature component in the top view, which leads to the delayed onset of the pinch-off in the cross junction. Furthermore, the influence of the opposite flow is shown in flow pattern maps built in terms of the flow rate ratio and the normalized droplet length. To explain the adjustable functions of the cross junction at different flow rate ratios, two different mechanisms are proposed.

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Hydrodynamic dissection ofStentor coeruleusin a microfluidic cross junction

Abstract: Stentor coeruleus, a single-cell ciliated protozoan, is a model organism for wound healing and regeneration studies. Despite Stentor’s large size (up to 2 mm in extended state), microdissection of Stentor...

Cited by 4 publications

References 68 publications

Recent advances in deformation-assisted microfluidic cell sorting technologies

Recent advances in deformation-assisted microfluidic cell sorting technologies

Vortex-induced particle capture in a micro cross-shaped channel

Adjustable behaviors and dynamic mechanisms of droplets in the cross junction

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