Due to their small dimensions, microfluidic devices operate in the low Reynolds number regime. In this case, the hydrodynamics is governed by the viscosity rather than inertia and special elements have to be introduced into the system for mixing and pumping of fluids. Here we report on the realization of an effective pumping device that mimics a ciliated surface and imitates its motion to generate fluid flow. The artificial biomimetic cilia are constructed as long chains of spherical superparamagnetic particles, which selfassemble in an external magnetic field. Magnetic field is also used to actuate the cilia in a simple nonreciprocal manner, resulting in a fluid flow. We prove the concept by measuring the velocity of a cilia-pumped fluid as a function of height above the ciliated surface and investigate the influence of the beating asymmetry on the pumping performance. A numerical simulation was carried out that successfully reproduced the experimentally obtained data.biomimetics | microfluidics | colloids | low Reynold's number | hydrodynamics E fficient pumping and mixing of fluids in microscopic channels is paramount in microfluidic applications (1, 2). Small characteristic dimensions of such devices result in very low Reynolds numbers and one encounters hydrodynamics that is conceptually different from the turbulent macroscopic world. As stated by Purcell's "scallop theorem," nonreciprocal motion is required for generation of fluid flow or directed swimming (3). This is clearly manifested in biological systems, for instance in bending waves of sperm tails or in corkscrew motion of bacterial flagella. Another example are cilia, flexible protrusions on the surface of many eukaryotic cells with a typical length of several micrometers. In humans, ciliated surfaces are found, for example, in the respiratory tract where they sweep mucus, or in the Fallopian tubes where they move an ovum to the uterus. The motion of the fluid above a ciliated surface is generated by periodic beating of cilia. Experimental observations have shown that the beating pattern of an individual cilium is asymmetric and composed of two phases: the effective stroke, during which the outstretched cilium propels the fluid like an oar, followed by the recovery stroke, when the bent cilium returns to the initial position sweeping along the surface in a way that produces as little backward flow as possible (4). Although each cilium can beat independently, cilia densely covering a surface synchronize their cycles and form metachronal waves, thus increasing their fluid pumping efficiency (5). It is believed that the metachronal waves occur as a result of hydrodynamical interactions between the cilia (6, 7).The efficiency of the ciliary pumping mechanism leads to the idea of using the same principle for designing artificial cilia that act as microscale pumps and mixers. An important step towards biomimetic cilia was made by Darnton et al., who created a bacterial carpet by attaching bacteria to a solid surface (8). Due to symmetric rotation and wea...