Hydrodynamic separation of particles using pinched‐flow fractionation

Ashley, John F.; Bowman, Christopher N.; Davis, Robert H.

doi:10.1002/aic.14087

Cited by 17 publications

(19 citation statements)

References 33 publications

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“…Copyright 2005 The Royal Society of Chemistry.). As illustrated in (iii) [ 21 ], the smaller particle will closely follow the fluid streamline and move toward the upper portion of the exit area, while the larger particles move closer to the center. This is due to the smaller cells tends to move faster under hydrodynamic force which resulted them to press closer to the wall as the flow ratio increase (Reprinted with permission from [ 21 ].…”

Section: Figurementioning

confidence: 99%

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

Low

Abas

2015

BioMed Research International

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Circulating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. The importance of CTCs as prognostic biomarker is leveraged when multiple studies found that patient with cutoff of 5 CTCs per 7.5 mL blood has poor survival rate. Despite its clinical relevance, the isolation and characterization of CTCs can be quite challenging due to their large morphological variability and the rare presence of CTCs within the blood. Numerous methods have been employed and discussed in the literature for CTCs separation. In this paper, we will focus on label free CTCs isolation methods, in which the biophysical and biomechanical properties of cells (e.g., size, deformability, and electricity) are exploited for CTCs detection. To assess the present state of various isolation methods, key performance metrics such as capture efficiency, cell viability, and throughput will be reported. Finally, we discuss the challenges and future perspectives of CTC isolation technologies.

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Section: Figurementioning

confidence: 99%

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

Low

Abas

2015

BioMed Research International

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“…In contrast to these methods, there are passive technologies designed based on hydrodynamic forces which can be associated with novel geometries for microchannels to improve the channel efficiency. Passive methods include inertial microfluidics (Amini et al 2014;Di Carlo 2009;Di Carlo et al 2007;Kim et al 2018;Martel and Toner 2014), deterministic lateral displacement (Huang et al 2004;Loutherback et al 2010) and pinched flow fractionation (Ashley et al 2013;Yamada et al 2004). Using the active methods, the Reynolds number must be kept sufficiently low for the channel so that the external field would be practically effective.…”

Section: Introductionmentioning

confidence: 99%

Inertial microfluidics: Determining the effect of geometric key parameters on capture efficiency along with a feasibility evaluation for bone marrow cells sorting

Ghadiri

Hosseini

Sadatsakkak

et al. 2021

Biomed Microdevices

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Despite great developments in inertial microfluidics, there is still a lack of knowledge to precisely define the particles' behavior in the microchannels. In the present study, as a prerequisite to experimental studies, numerical simulations have been used to study the capture efficiency of target particles in the contraction-expansion microchannel, aiming to provide an estimation of the conditions at which the channel performs best. Fluid analysis based on Navier-Stokes equations is conducted using the finite element method to determine the streamlines and vortices. The highest capture efficiency for 10, 15, and 19-micron particles occurs when the center of the vortex is approximately in the middle of the wide section (at the flow rate of 0.35 ml/ min). In addition to investigating the effect of particle diameter and input flow rate, the effect of channel geometry parameters (channel height and initial length of the channel) on particle trapping has also been studied. Also, to consider great interest in separating different-sized bioparticles from a sample, a three-stage platform has been designed to separate four types of bone marrow cells and evaluate the possibility of using contraction-expansion channels in this application.

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“…Two factors indicate the importance of considering hydrodynamic interactions to determine the particle trajectories through a constriction. First, the particles are similar in size to the width of the constriction and, therefore, cannot be considered as tracer particles advected by the flow [1,16,21]. Second, as the particles move through the constriction, the surface-to-surface separation between the particles and the channel wall tend to becomes much smaller that the size of the particles, and lubrication forces can play a significant role.…”

Section: Introductionmentioning

confidence: 99%

Mechanism governing separation in microfluidic pinched flow fractionation devices

Risbud

Drazer

2014

Microfluid Nanofluid

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We present a computational investigation of the mechanism governing size-based particle separation in microfluidic pinched flow fractionation. We study the behavior of particles moving through a pinching gap (i.e., a constriction in the aperture of a channel) in the Stokes regime (negligible fluid and particle inertia) as a function of particle size. The constriction aperture is created by a plane wall and spherical obstacle, and emulates the pinching segment in pinched flow fractionation devices. The simulation results show that the distance of closest approach between the particle and obstacle surfaces (along a trajectory) decreases with increasing particle size. We then use the distance of closest approach to investigate the effect of short-range repulsive non-hydrodynamic interactions (e.g., solid-solid contact due to surface roughness, electrostatic or steric repulsion, etc.). We define a critical trajectory as the one in which the minimum particle-obstacle separation is equal to the range of the non-hydrodynamic interactions. The results further show that the initial offset of the critical trajectory (defined as the critical offset) increases with particle size. We interpret the variation of the critical offset with particle size as the basis for size-based microfluidic separation in pinched flow fractionation. We also compare the effect of different driving fields on the particle trajectories;we simulate a constant force driving the particles in a quiescent fluid as well as a freely suspended particles in a pressure-driven flow. We observe that the particles driven by a constant force approach closer to the obstacle than those suspended in a flow (for the same initial offset). On the other hand, the increment in the critical offset (as a function of particle size) is larger in the pressure-driven case than in the force-driven case. Thus, pressure-driven particle separation using pinched flow fractionation would prove more effective than its force-driven counterpart (e.g., particles settling under gravity through a pinching gap).

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Hydrodynamic separation of particles using pinched‐flow fractionation

Cited by 17 publications

References 33 publications

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

Inertial microfluidics: Determining the effect of geometric key parameters on capture efficiency along with a feasibility evaluation for bone marrow cells sorting

Mechanism governing separation in microfluidic pinched flow fractionation devices

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