Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes

Risbud, Makarand V.; Karamuk, Erdal; Schlosser, Viola; Mayer, Joerg

doi:10.1163/156856203322274969

Cited by 38 publications

(17 citation statements)

References 20 publications

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“…Our observations indicated that nitrocellulose membranes supported HepG2 cell attachment, morphology, proliferation, and function, which were similar to the results reported in other studies (Kataoka et al 2005;Risbud et al 2003;Kasoju et al 2009;She et al 2008), suggesting that we should further explore the application of nitrocellulose membranes in membrane bioreactors. Recently, several new membrane materials have been used in bioreactors, such as cuprophane, cellulose acetate, polysulfone, and polypropylene membranes.…”

Section: Discussionsupporting

confidence: 90%

Use of nitrocellulose membranes as a scaffold in cell culture

Wang

Deng

et al. 2012

Cytotechnology

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Nitrocellulose membranes, one of the most important and oldest cellulose derivatives, are commonly used for nucleic acid and protein detection in research and diagnostic applications. However, a limited number of studies have explored whether they can act as scaffolds for cell growth. In this study, we investigated this polymeric material for its ability to support the growth of human cells. Eight established cell lines were examined for adherence, growth, spread, and survival on nitrocellulose membranes by optical microscopy after hematoxylin and eosin and/or immunocytochemical staining and by scanning electron microscopy. Apoptosis and leakage of lactate dehydrogenase (LDH) were also assessed. All cells readily adhered to and spread on the surface of nitrocellulose membranes as well as coverslips, and the cells maintained the expression of digestive system-specific genes. No significant change was detected in apoptosis or leakage of LDH from cells grown on nitrocellulose membranes. These results suggested that nitrocellulose membranes have a suitable cytocompatibility towards human cells and that they might be used for tissueengineering scaffolds. Moreover, we demonstrate an additional and underused property of nitrocellulose of specific relevance to microscopic imaging, as it can be rendered virtually transparent, thus the cells growing on such membranes can be observed directly under an optical microscope after staining.

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Section: Discussionsupporting

confidence: 90%

Use of nitrocellulose membranes as a scaffold in cell culture

Wang

Deng

et al. 2012

Cytotechnology

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“…[41][42][43][44][45][46][47][48][49] Because of their unique properties, hydrogels have been explored for a wide range of biomedical applications, including drug delivery, 50 protein delivery, 51 gene delivery, 52 wound dressing, 53 water elimination for edema, 54 cosmetic reconstruction, 55 contact lenses, 56 and tissue engineering. 49,[57][58][59] One major challenge in employing hydrogels for load-bearing connective tissue repair in cartilage, tendon, intervertebral disk, ligament, and so on, is insufficient mechanical strength. For instance, articular cartilage contains *70% water and bears loads up to 100 MPa, 60 but most hydrogels, either synthetic or derived from natural sources, can be easily broken by pressing with a finger or pulling with the hand, indicating that they are much weaker than native cartilage tissue.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and characterization of biocompatible, degradable, light‐curable, polyurethane‐based elastic hydrogels

Zhang

Wen

2007

J Biomedical Materials Res

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A series of degradable polyurethane-based light-curable elastic hydrogels were synthesized from polycaprolactone diol, polyethylene glycol (PEG), lysine diisocyanate (LDI), and 2-hydroxyethyl methacrylate (HEMA) through UV light initiated polymerization reaction. LDI was used as hard segment and polycaprolactone (PCL) and/or PEG were used as soft segments. By changing the PCL to PEG ratio during the prepolymer synthesis, polyurethanes with different soft segmental structures, hydrophilicity, and cytophilicity were obtained after light-initiated polymerization. The chemical structures of the synthesized polymers were characterized using differential scanning calorimetry and Fourier transform infrared spectroscopy. Physical properties such as swelling, mechanical properties, and in vitro degradation were evaluated. Materials containing a higher ratio of PEG exhibit higher water absorbance, higher degradation rate in vitro, and lower mechanical strength in the hydrated state. Mouse embryonal carcinoma-derived clonal chondrocytes were used as a model cell type to study the cytocompatibility of the synthesized polymers. Chondrocyte attachment, proliferation rates, and morphologies varied with changes in the PCL/PEG ratio. With a higher PEG ratio, lower cell attachment and proliferation were observed. To improve the cell attachment and proliferation on high PEG content hydrogels, bioactive molecules, such as peptides and proteins, were conjugated or immobilized in the gel matrix during the light-curing process. In this study, a short peptide, Arg-Gly-Asp-Ser, was used as a model biomolecule and incorporated into the gels during the light-curing process and improved cell growth was observed. In summary, the use of PCL/PEG at different ratios, as well as the introduction of HEMA into polyurethane, allows the synthesis of a series of biocompatible elastic hydrogels with tunable physical and cytophilic properties through light-initiated polymerization. This series of materials also allows for controlling cell attachment and growth by incorporating bioactive molecules during the light-curing process.

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“…Hence for rapid hydration and regeneration of skin, aloe is intended to be used in this formulation to make the skin smooth and elastic. Though many studies have shown the incompatibility of skin fibroblasts with chitosan, it has been reported that the addition of collagen facilitates the attachment and proliferation of skin fibroblasts [34][35][36][37] .…”

Section: Fig 2: Sa-β-gal Positive Nih3t3 Mouse Fibro Blasts Cells (Cmentioning

confidence: 99%

Untitled

2018

IJPSR

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Photochemoprotection has nowadays become inevitable to combat aging. Exposure to Ultraviolet radiations accelerates skin aging which eventually culminates in wrinkles, laxity, dyspigmentation, roughness and dryness. Though many active synthetic topicals have been used since years, the present era of treating an aged skin has been diverted towards natural biomaterials as these synthetic topicals pose health and safety risk on human health. In an effort to produce a promising natural anti-aging agent in contemporary cosmetics, this study has been aimed to formulate an anti-aging gel by blending three biopolymers namely Collagen (COL) 3%w/v, Chitosan (CS) 1.5% w/v and Aloe vera (AV) gel 0.21% w/v. The AV blended COL-CS gel was characterized by spreadability and moisture uptake test, which indicated good spreadability and increased hydrophyllicity of the gel. Cell culture studies on NIH3T3 mouse fibroblasts cells were carried out using senescenceassociated-β-gal [SA-β-gal] as a biomarker. The fibroblasts cells on incubation with AV blended COL-CS gel showed increased proliferation rate and the absence of blue stain indicated that the process of senesce has been reversed. In conclusion, the prepared AV blended COL-CS gel helps in regeneration and rejuvenation of the skin and therefore can be used as a promising anti-aging gel.

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Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes

Cited by 38 publications

References 20 publications

Use of nitrocellulose membranes as a scaffold in cell culture

Use of nitrocellulose membranes as a scaffold in cell culture

Synthesis and characterization of biocompatible, degradable, light‐curable, polyurethane‐based elastic hydrogels

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