Hydrogel Encapsulated Core–Shell Photonic Barcodes for Multiplex Biomarker Quantification

Abstract

Acute myocardial infarction (AMI) is one of the most fatal diseases in the world in recent decades. Because rapid and accurate determination of AMI has the potential to save millions of lives globally, the development of new diagnostic method is of great significance. Here, we designed a magnetic responsive structural color core−shell hydrogel microcarrier as a novel platform for a high-throughput detection of a variety of cardiovascular biomarkers. The composite hydrogel shell was formed from methacrylated gelatin, acrylic acid, and poly(ethylene glycol diacrylate), and the core silica photonic crystals acted as a detector. Fe 3 O 4 nanoparticles were infused into the void of the core−shell structure to impart magnetic response properties to the encoded carrier. The findings indicated that our method possessed high sensitivity and reliable specificity in the high-throughput detection of AMI-related biomarkers Myo, cTnI, and BNP. In addition, the developed method not only showed good specificity and high sensitivity in clinical samples but also was comparable to the clinical gold standard method. Therefore, the magnetic response structural color core−shell hydrogel carriers were regarded as a potential approach to detect some AMI disease-related biomarkers.

Multiplex Aptamer-Based Fluorescence Assay Using Magnetism-Encoded Nanoparticles for Simultaneous Detection of Multiple Pathogenic Bacteria

“…Multiplex assays are analytical techniques that allow for the simultaneous detection and quantification of multiple analytes in a single test. Compared to traditional single-analyte assays, multiplex assay offers the advantages of improved efficiency, cost-effectiveness, and conservation of limited sample volume. , More importantly, it enables a more comprehensive analysis and a deeper understanding of complex biological systems and processes. − Accordingly, considerable efforts have been devoted to the development of diverse multiplex assays. − However, current research in this field primarily focuses on the design and fabrication of various detection labels that produce distinguishable signals. − Among these labels, fluorescence is the most widely used due to its rapid response, high sensitivity, and low detection limit. Indeed, many multiplex fluorescence assays have been developed and successfully applied for the simultaneous detection of a wide range of analytes such as bacteria, nucleic acids, and toxins .…”

Bioinspired Confined Assembly of Cellulosic Cholesteric Liquid Crystal Bubbles

“…[ 1 , 2 , 3 , 4 ] Due to its intrinsic coloration mechanism, structural color is stable and easy‐perceivable, and these advantages have long been an impetus for the manufacture of artificial structural color materials. [ 5 , 6 , 7 , 8 ] Through either microfabrication methods or self‐assembly of basic units, such as colloidal nanoparticles and block copolymers, structural color materials with various configurations and functions have been constructed, showing extensive application values in anti‐counterfeiting, optical display, sensing, and biomedical devices. [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ] Although numerous efforts have been made, a large portion of them focus on creating solid photonic structures including microgrooves, stackings, three‐dimensional colloidal crystals, [ 16 , 17 , 18 , 19 , 20 , 21 ] etc.…”

A rapid screening platform for antibiotic susceptibility testing based on a simple colorimetric method

“…For a phenotypic category, the bacteria susceptible to antibiotics are directly observed by exposing them to anti-biotics. Microfluidics is a phenotypic category antibiotic susceptibility testing method, and the test time could be less than 3 h. [22][23][24] However, the initial concentration of microfluidics is high, which requires a pretreatment enrichment process, and the production cost of microfluidics is high.…”