Hydrogel hybrid porcine pericardium for the fabrication of a pre-mounted TAVI valve with improved biocompatibility

Guo, Guanhua; Jin, Wanyu; Jin, Linhe; Chen, Liang; Lei, Yang; Wang, Yunbing

doi:10.1039/c8tb02565g

Cited by 16 publications

(23 citation statements)

References 41 publications

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“…In contrast, the surface ζ potential of the heart valve group modified with RBC/RAPA&AC@PLGA nanoparticles was very stable (from −8.6 to −10.0 mV) at pH values ranging from 3.9 to 8.1, the surface ζ potential was about −8 mV when the pH was 7.4. The weight-loss ratio of heart valves after being treated with collagenase was used to assess the protection of collagen . GLU, RBC/PLGA, RBC/RAPA@PLGA, RBC/AC@PLGA, and RBC/RAPA&AC@PLGA groups (Figure G) emerged with weight-loss ratios of 9.37, 9.69, 9.19, 8.89, and 9.72%, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The weight-loss ratio of heart valves after being treated with collagenase was used to assess the protection of collagen. 21 GLU, RBC/PLGA, RBC/ RAPA@PLGA, RBC/AC@PLGA, and RBC/RAPA&AC@ PLGA groups (Figure 1G) emerged with weight-loss ratios of 9.37, 9.69, 9.19, 8.89, and 9.72%, respectively. This result did not show large differences in the weight-loss percentages compared to the GLU group, demonstrating that the nanoparticle modification did not change the collagen stability of the heart valves.…”

Section: Resultsmentioning

confidence: 99%

“…GLU-treated BHVs could inhibit the adhesion and proliferation of endothelial cells due to the high toxicity of glutaraldehyde, which is closely related to the aldehyde groups of the valve . Still, the GLU-treated BHVs revealed the disadvantages such as immune response, valvular thrombosis, endothelialization difficulty, calcification seriousness, and a shorter life-span of the valve (generally only 10–15 years) due to altered mechanical properties and compromised functionality. , The short life span of the heart valves treated with glutaraldehyde is also detrimental for young patients who need much longer durability of the implanted valves. Therefore, it is the key point to improve the durability, safety, and life span of BHVs.…”

Section: Introductionmentioning

confidence: 99%

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Heart Valves Cross-Linked with Erythrocyte Membrane Drug-Loaded Nanoparticles as a Biomimetic Strategy for Anti-coagulation, Anti-inflammation, Anti-calcification, and Endothelialization

Luo

Wang

2020

ACS Appl. Mater. Interfaces

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In recent years, valvular heart disease has become a serious disease threatening human life and is a major cause of death worldwide. However, the glutaraldehyde (GLU)-treated biological heart valves (BHVs) fail to meet all requirements of clinical application due to disadvantages such as valve thrombus, cytotoxicity, endothelialization difficulty, immune response, and calcification. Encouragingly, there are a large number of carboxyls as well as a few amino groups on the surface of GLU-treated BHVs that can be modified to enhance biocompatibility. Inspired by natural biological systems, we report a novel approach in which the heart valve was cross-linked with erythrocyte membrane biomimetic drug-loaded nanoparticles. Such modified heart valves not only preserved the structural integrity, stability, and mechanical properties of the GLU-treated BHVs but also greatly improved anti-coagulation, anti-inflammation, anti-calcification, and endothelialization. The in vitro results demonstrated that the modified heart valves had long-term anti-coagulation properties and enhanced endothelialization processes. The modified heart valves also showed good biocompatibility, including blood and cell biocompatibility. Most importantly, the modified heart valves reduced the TNF-α levels and increased IL-10 compared to GLU-treated BHVs. In vivo animal experiments also confirmed that the modified heart valves had an ultrastrong resistance to calcification after implantation in rats for 120 days. The mechanism of anti-calcification in vivo was mainly due to the controlled release of anti-inflammatory drugs that reduced the inflammatory response after valve implantation. In summary, this therapeutic approach based on BHVs cross-linking with erythrocyte membrane biomimetic nanoparticles sparks a novel design for valvular heart disease therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Heart Valves Cross-Linked with Erythrocyte Membrane Drug-Loaded Nanoparticles as a Biomimetic Strategy for Anti-coagulation, Anti-inflammation, Anti-calcification, and Endothelialization

Luo

Wang

2020

ACS Appl. Mater. Interfaces

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“…Radical polymerization was widely used to prepare functional biomaterials such as hydrogels due to the diversity of monomers and mild reaction conditions. 20 Our lab developed radical polymerization method to crosslink porcine pericardium and showed superior anti-calcification properties. This new method avoided the use of toxic aldehyde groups.…”

Section: Discussionmentioning

confidence: 99%

Glycidyl methacrylate-crosslinked fish swim bladder as a novel cardiovascular biomaterial with improved antithrombotic and anticalcification properties

Cheng

et al. 2021

J Biomater Appl

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At present, commercial artificial biological valves are mostly prepared by crosslinking bovine or porcine pericardia with glutaraldehyde. Swim bladder has similar components and lower immunogenicity compared to bovine or porcine pericardium. In this study, we used a glycidyl methacrylate (GMA)–based radical polymerization method to crosslink decellularized swim bladders. Amino and carboxyl groups in the swim bladder were reacted with epoxy groups on GMA to introduce carbon–carbon double bonds to the swim bladder. The results showed that the platelet adhesion of GMA-crosslinked swim bladders (GMA-SBs) decreased by 35%, as compared to that of glutaraldehyde-crosslinked swim bladders (GLUT-SBs). Moreover, the superior anticoagulant property was further verified by the ex vivo arteriovenous shunt assay. Meanwhile, the subcutaneous implantation in rats showed that GMA-SBs were able to effectively inhibit the calcification compared with GLUT-SBs. In conclusion, GMA-SBs showed improved antithrombotic and anticalcification properties compared to GLUT-SBs.

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“…Hydrogel coating has been utilized to improve anti‐crimp properties of commercialized BHV made from porcine pericardium (PP) after long‐term folding. [ 20 ] Meanwhile, different hydrogels can be designed and selected based on the need for biomedical functions. [ 21 ] However, for antifatigue properties, homogeneous single network hydrogels are relatively weak, which leads to rapid damage during cyclic hydrodynamic stress.…”

Section: Introductionmentioning

confidence: 99%

Double‐Network Hydrogel Armored Decellularized Porcine Pericardium as Durable Bioprosthetic Heart Valves

Cheng

Liu

Qian

et al. 2022

Adv Healthcare Materials

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Heart valves have extraordinary fatigue resistance which beat ≈3 billion times in a lifetime. Bioprosthetic heart valves (BHVs) made from fixed heteroplasm that are incrementally used in heart valve replacement fail to sustain the expected durability due to thrombosis, poor endothelialization, inflammation, calcification, and especially mechanical damage induced biocompatibility change. No effective strategy has been reported to conserve the biological properties of BHV after long-term fatigue test. Here, a double-network tough hydrogel is introduced, which interpenetrate and anchor into the matrix of decellularized porcine pericardium (dCell-PP) to form robust and stable conformal coatings and reduce immunogenicity. The ionic crosslinked hyaluronic acid (HA) network mimics the glycocalyx on endothelium which improves antithrombosis and accelerates endothelialization; the chemical crosslinked hydrophilic polyacrylamide (PAAm) network further enhances antifouling properties and strengthens the shielding hydrogels and their interaction with dCell-PP. In vitro and rabbit ex vivo shunt assay demonstrate great hemocompatibility of polyacrylamide/HA hydrogel hybrid PP (P/H-PP). Cell experiments and rat subcutaneous implantation confirm satisfactory endothelialization, biocompatibility, and anticalcification properties. For hydrodynamic experiment, P/H-PP gains full mark at different flow conditions and sustains excellent biomechanical and biological properties after 200 000 000 cycles. P/H double-network hydrogel armoring dCell-PP is a promising progress to extend BHV durability for clinical implantation therapy.

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Hydrogel hybrid porcine pericardium for the fabrication of a pre-mounted TAVI valve with improved biocompatibility

Abstract: Hydrogel/porcine pericardium hybrid provides a more biocompatible biomaterial for the pre-mounted TAVI valve.

Cited by 16 publications

References 41 publications

Heart Valves Cross-Linked with Erythrocyte Membrane Drug-Loaded Nanoparticles as a Biomimetic Strategy for Anti-coagulation, Anti-inflammation, Anti-calcification, and Endothelialization

Heart Valves Cross-Linked with Erythrocyte Membrane Drug-Loaded Nanoparticles as a Biomimetic Strategy for Anti-coagulation, Anti-inflammation, Anti-calcification, and Endothelialization

Glycidyl methacrylate-crosslinked fish swim bladder as a novel cardiovascular biomaterial with improved antithrombotic and anticalcification properties

Double‐Network Hydrogel Armored Decellularized Porcine Pericardium as Durable Bioprosthetic Heart Valves

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