Hydrogel increases localized transport regions and skin permeability during low frequency ultrasound treatment

Pereira, Tatiana Aparecida; Ramos, Daniela; Lopez, Renata Fonseca Vianna

doi:10.1038/srep44236

Cited by 36 publications

(28 citation statements)

References 44 publications

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“…On the other hand, the non-ionic Poloxamer gel, which presents a thermosensitive behavior with increased viscosity as a function of increase in the temperature that occurs during the application of the ultrasound, was the one that presented the highest percentage of LTRs. Using the hydrogels as coupling media, LTRs extension was at least 3-fold greater than those observed using the traditional ultrasound treatment with SLS, suggesting a correlation between viscosity increase and LTRs formation (Pereira, Ramos, Lopez, 2017).…”

Section: Low Frequency Sonophoresismentioning

confidence: 82%

“…Recently, the influence of hydrogels as coupling medium and their impact in LTRs distribution after treatment with 20 kHz ultrasound was studied (Pereira, Ramos, Lopez, 2017). Porcine skin was ultrasound treated in vitro using cationic, anionic and non-ionic hydrogels with similar viscosities.…”

Section: Low Frequency Sonophoresismentioning

confidence: 99%

“…(Kigasawa et al, 2010) of the drug recovered from viable epidermis by 2.8-fold, whereas using the anionic hydrogel medium, the amount of DOX retained in the SC increased by approximately 4-fold. As a result, authors suggested that low-frequency ultrasound can potentially target ionized drugs to specific skin layers, by variations in the charge of the coupling medium (Pereira, Ramos, Lopez, 2017). The low and medium frequency ultrasound have been studied in association with different molecules for the treatment of inflammation (Cohen et al, 2015;Chan, Tong, Ng, 2016).…”

Section: Low Frequency Sonophoresismentioning

confidence: 99%

See 2 more Smart Citations

Physical methods for topical skin drug delivery: concepts and applications

Petrilli

Lopez

2018

Braz. J. Pharm. Sci.

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Topical drug delivery is an interesting approach to treat skin diseases and to avoid pain and low patient compliance in cases where a systemic delivery is required. However, the stratum corneum, which is the outermost skin layer, strongly protects the body from the entrance of substances, especially those hydrophilic. In this context, different physical methods have been studied to overcome the stratum corneum barrier and facilitate penetration of drugs into or through the skin. Among them, iontophoresis, low-frequency ultrasound and microneedles have been widely employed for transdermal drug delivery. More recently, they are also studied to aid in the treatment of dermatological disorders, such as skin tumors and inflammation. Basically, iontophoresis refers to the movement of charged and non-charged hydrophilic molecules through the skin due to the application of a low constant electric current and the contributions of electromigration and electroosmosis. In low-frequency ultrasound, cavitation is the main mechanism for skin permeabilization that consists on the formation of microbubbles that disorganize the stratum corneum. Microneedles are microprojections, minimally invasive, that can be designed with different lengths, materials and geometry to increase skin permeability. In this review, concepts, mechanisms and applications of these three physical methods will be presented and discussed with focus on their use in dermatological treatments. Moreover, comparative studies using different physical methods will be presented and also some clinical perspectives will be addressed.

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Section: Low Frequency Sonophoresismentioning

confidence: 82%

Section: Low Frequency Sonophoresismentioning

confidence: 99%

Section: Low Frequency Sonophoresismentioning

confidence: 99%

See 1 more Smart Citation

Physical methods for topical skin drug delivery: concepts and applications

Petrilli

Lopez

2018

Braz. J. Pharm. Sci.

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“…The coupling media were aerated for 20 min and 1 mL of each preparation was exposed to LFU (Sonics & Materials, VCX 500, Newtown, CT USA) operating at 20 kHz, spatial average temporal average intensity (I sata ) of 10 ± 0.5 W/cm 2 , 50% duty cycle (5 s on, 5 s off) for 1 min. The intensity of LFU was measured using calorimetry 36,37 at a duty cycle of 100%. The absorbance for KI 3 was measured at 350 nm after a 1:3 dilution of the samples in triplicate at room temperature using a Shimadzu spectrophotometer (UV 1800, Kyoto, Japan).…”

Section: Cavitational Activity Of Dspe-peg Micelles Potassium Iodide mentioning

confidence: 99%

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Martins¹,

Fonseca²,

Pavan³

et al. 2020

IJN

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Purpose: Sonodynamic therapy (SDT) is a new therapeutic modality for the noninvasive cancer treatment based on the association of ultrasound and sonosensitizer drugs. Topical SDT requires the development of delivery systems to properly transport the sonosensitizer, such as zinc phthalocyanine (ZnPc), to the skin. In addition, the delivery system itself can participate in sonodynamic events and influence the therapeutic response. This study aimed to develop ZnPc-loaded micelle to evaluate its potential as a topical delivery system and as a cavitational agent for low-frequency ultrasound (LFU) application with the dual purpose of promoting ZnPc skin penetration and generating reactive oxygen species (ROS) for SDT. Methods: ZnPc-loaded micelles were developed by the thin-film hydration method and optimized using the Quality by Design approach. Micelles' influence on LFU-induced cavitation activity was measured by potassium iodide dosimeter and aluminum foil pits experiments. In vitro skin penetration of ZnPc was assessed after pretreatment of the skin with LFU and simultaneous LFU treatment using ZnPc-loaded micelles as coupling media followed by 6 h of passive permeation of ZnPc-loaded micelles. The singlet oxygen generation by LFU irradiation of the micelles was evaluated using two different hydrophilic probes. The lipid peroxidation of the skin was estimated using the malondialdehyde assay after skin treatment with simultaneous LFU using ZnPc-loaded micelles. The viability of the B16F10 melanoma cell line was evaluated using resazurin after treatment with different concentrations of ZnPc-loaded micelles irradiated or not with LFU. Results: The micelles increased the solubility of ZnPc and augmented the LFU-induced cavitation activity in two times compared to water. After 6 h ZnPc-loaded micelles skin permeation, simultaneous LFU treatment increased the amount of ZnPc in the dermis by more than 40 times, when compared to non-LFU-mediated treatment, and by almost 5 times, when compared to LFU pretreatment protocol. The LFU irradiation of micelles induced the generation of singlet oxygen, and the lipoperoxidation of the skin treated with the simultaneous LFU was enhanced in three times in comparison to the non-LFU-treated skin. A significant reduction in cell viability following treatment with ZnPc-loaded micelles and LFU was observed compared to blank micelles and non-LFU-treated control groups. Conclusion: LFU-irradiated mice can be a potential approach to skin cancer treatment by combining the functions of increasing drug penetration and ROS generation required for SDT.

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“…Pereira et al 31 delivered doxorubicin transdermally into porcine skin in vitro using ultrasound, while attempting to enhance skin permeability through the application of various coupling mediums. These solutions included poloxamer, hydroxyethyl cellulose, chitosan, and carbopol hydrogels, in addition to sodium lauryl sulfate solution.…”

Section: Single-frequency Sp Of Drugsmentioning

confidence: 99%

Recent advances in ultrasound-based transdermal drug delivery

Seah¹,

Teo²

2018

IJN

120

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The transdermal transport of pharmaceuticals possesses various advantageous properties over conventional drug administration techniques such as oral delivery and hypodermic injections. However, the stratum corneum persists as the main barrier, which impedes percutaneous transport. The ultrasound-based transdermal delivery of therapeutics is one of the techniques that are being investigated to overcome this obstacle. This review outlines the background information pertaining to sonophoresis and then discusses the individual sections of sonophoretic research. These areas include the sonophoretic application of various drugs, dual-frequency sonophoresis, synergistic combinations of transdermal drug delivery techniques, and the use of nanosized carriers in ultrasound-based transdermal delivery. The various challenges associated with sonophoretic drug delivery and trends of future research are also highlighted.

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Hydrogel increases localized transport regions and skin permeability during low frequency ultrasound treatment

Cited by 36 publications

References 44 publications

Physical methods for topical skin drug delivery: concepts and applications

Physical methods for topical skin drug delivery: concepts and applications

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Recent advances in ultrasound-based transdermal drug delivery

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