Hydrogel microfabrication technology toward three dimensional tissue engineering

Yanagawa, Fumiki; Sugiura, Shinji; Kanamori, Toshiyuki

doi:10.1016/j.reth.2016.02.007

Cited by 116 publications

(87 citation statements)

References 147 publications

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“…In these conditions, limitations on polymerization due to light attenuation are not significant. Although other applications such as nerve guidance require thicker constructs, fabrication techniques such as microencapsulation and microextrusion can assist producing smaller cell loaded devices without compromising their function …”

Section: Resultsmentioning

confidence: 99%

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Aregueta‐Robles

Martens

Poole-Warren

et al. 2017

J Polym Sci B Polym Phys

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State‐of‐the‐art neurorprostheses rely on stiff metallic electrodes to communicate with neural tissues. It was envisioned that a soft, organic electrode coating embedded with functional neural cells will enhance electrode‐tissue integration. To enable such a device, it is necessary to produce a cell scaffold with mechanical properties matched to native neural tissue. A degradable poly(vinyl alcohol) (PVA) hydrogel was tailored to have a range of compressive moduli through variation in macromer composition and initiator amount. A regression model was used to predict the amount of initiator required for hydrogel polymerization with nominal macromer content ranging between 5 and 20 wt %. Hydrogels at 5 and 10 wt % were reliably formed but 15 wt % and above were not able to be fabricated due to the light attenuation properties of the initiator ruthenium at increased concentration. Compressive modulus of hydrogels decreased upon incorporation of biomolecules (sericin and gelatin), however, the bulk stiffness spanned the range required to match neural tissue properties (0.04–20kPa). Neuroglia cells, such as Schwann cells survived and grew within the scaffold. The significant finding of this work is that the PVA‐tyramine system can be tuned to provide a soft degradable scaffold for neural tissue regeneration while presenting bioactive molecules for cellular expansion. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2018, 56, 273–287

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Section: Resultsmentioning

confidence: 99%

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Aregueta‐Robles

Martens

Poole-Warren

et al. 2017

J Polym Sci B Polym Phys

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“…Cell-laden hydrogel microcapsules could be fabricated in multiple ways 36 . Electrospraying, which takes advantage of electric fields and Rayleigh-Plateau instability, is conventionally used to generate cell-laden microdroplets and hydrogel microcapsules 37, 38 .…”

Section: Introductionmentioning

confidence: 99%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

et al. 2017

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Hydrogel microcapsules provide miniaturized and biocompatible niches for three-dimensional (3D) in vitro cell culture. They can be easily generated by droplet-based microfluidics with tunable size, morphology, and biochemical properties. Therefore, microfluidic generation and manipulation of cell-laden microcapsules can be used for 3D cell culture to mimic the in vivo environment towards applications in tissue engineering and high throughput drug screening. In this review of recent advances mainly since 2010, we will first introduce general characteristics of droplet-based microfluidic devices for cell encapsulation with an emphasis on the fluid dynamics of droplet breakup and internal mixing as they directly influence microcapsule’s size and structure. We will then discuss two on-chip manipulation strategies: sorting and extraction from oil into aqueous phase, which can be integrated into droplet-based microfluidics and significantly improve the qualities of cell-laden hydrogel microcapsules. Finally, we will review various applications of hydrogel microencapsulation for 3D in vitro culture on cell growth and proliferation, stem cell differentiation, tissue development, and co-culture of different types of cells.

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“…Because cells interact with the ECM and other cells in the complicated 3D environment, the topography of the hydrogel should be considered to better mimic physiological environments. For this purpose, various techniques have been introduced, including soft lithography, fiber generation, laser microstructuration, replica molding, and bioprinting . Although such hydrogel structures show comparable structures and cellular functions as the in vivo tissues, higher resolution, easier fabrication, and mass production of such hydrogel structures are required for 3D cell culture and organ‐on‐a‐chip applications.…”

Section: Discussionmentioning

confidence: 99%

Hydrogel‐based three‐dimensional cell culture for organ‐on‐a‐chip applications

Lee

Shim

Kim

et al. 2017

Biotechnology Progress

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Recent studies have reported that three-dimensionally cultured cells have more physiologically relevant functions than two-dimensionally cultured cells. Cells are three-dimensionally surrounded by the extracellular matrix (ECM) in complex in vivo microenvironments and interact with the ECM and neighboring cells. Therefore, replicating the ECM environment is key to the successful cell culture models. Various natural and synthetic hydrogels have been used to mimic ECM environments based on their physical, chemical, and biological characteristics, such as biocompatibility, biodegradability, and biochemical functional groups. Because of these characteristics, hydrogels have been combined with microtechnologies and used in organ-on-a-chip applications to more closely recapitulate the in vivo microenvironment. Therefore, appropriate hydrogels should be selected depending on the cell types and applications. The porosity of the selected hydrogel should be controlled to facilitate the movement of nutrients and oxygen. In this review, we describe various types of hydrogels, external stimulation-based gelation of hydrogels, and control of their porosity. Then, we introduce applications of hydrogels for organ-on-a-chip. Last, we also discuss the challenges of hydrogel-based three-dimensional cell culture techniques and propose future directions. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:580-589, 2017.

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Hydrogel microfabrication technology toward three dimensional tissue engineering

Cited by 116 publications

References 147 publications

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Hydrogel‐based three‐dimensional cell culture for organ‐on‐a‐chip applications

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