Hydrogel Microparticles Functionalized with Engineered Escherichia coli as Living Lactam Biosensors

Ma, Conghui; Li, Jie; Zhang, Boyin; Liu, Chenxi; Zhang, Jingwei; Liu, Yifan

doi:10.3390/s19245556

Cited by 12 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrogels consist of cross‐linked hydrophilic polymers that can retain large amounts of water in their 3D networks, a feature that benefits cell growth (Ahmed, 2015 ). Among the hydrogels available, the commonly used are agarose and alginate gels (Ma et al, 2019 ). The ratio between the cells and the matrix must be properly optimized.…”

Section: Open Research Challenges For Yeast‐based Biosensorsmentioning

confidence: 99%

Biological and technical challenges for implementation of yeast‐based biosensors

Wahid

Ocheja

Marsili

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

Biosensors are low‐cost and low‐maintenance alternatives to conventional analytical techniques for biomedical, industrial and environmental applications. Biosensors based on whole microorganisms can be genetically engineered to attain high sensitivity and specificity for the detection of selected analytes. While bacteria‐based biosensors have been extensively reported, there is a recent interest in yeast‐based biosensors, combining the microbial with the eukaryotic advantages, including possession of specific receptors, stability and high robustness. Here, we describe recently reported yeast‐based biosensors highlighting their biological and technical features together with their status of development, that is, laboratory or prototype. Notably, most yeast‐based biosensors are still in the early developmental stage, with only a few prototypes tested for real applications. Open challenges, including systematic use of advanced molecular and biotechnological tools, bioprospecting, and implementation of yeast‐based biosensors in electrochemical setup, are discussed to find possible solutions for overcoming bottlenecks and promote real‐world application of yeast‐based biosensors.

show abstract

Section: Open Research Challenges For Yeast‐based Biosensorsmentioning

confidence: 99%

Biological and technical challenges for implementation of yeast‐based biosensors

Wahid

Ocheja

Marsili

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…For example, cell surfaces may be functionalized with thin material layers or objects which promote aggregation, [ 71 ] and cells themselves may be directly attached to larger material building blocks that confer cues of assembly or aggregation. [ 72 ] Composites created using these techniques have been implemented in areas such as biosensing, [ 73 ] water‐treatment, [ 74 ] bioremediation, [ 2b,75 ] drug delivery, [ 76 ] energy, [ 77 ] bioproduction, [ 78 ] “smart packaging,” [ 79 ] tissue engineering, [ 80 ] immune engineering, [ 81 ] and cell encapsulation. [ 82 ] Often, these composites are largely fixed in their final form; despite maintaining cellular viability and function, the overall 3D composite may be limited in functionality and demonstrate no perceptible changes over time.…”

Section: Composite Living Materialsmentioning

confidence: 99%

Hylozoic by Design: Converging Material and Biological Complexities for Cell‐Driven Living Materials with 4D Behaviors

Shariati

Ling

Fuchs

et al. 2021

Adv Funct Materials

View full text Add to dashboard Cite

The emergence of novel techniques in biology and material science, coupled with greater understandings of cell-material interactions, have given rise to the creation of living materials and bioarchitectures imbued with engineered living behaviors. These multidimensional materials and constructs capable of performing programmable and time-or stimuli-dependent activities, namely 4D behaviors, may do so as a result of material features alone, or as a product of incorporated biological or cellular agents. This review discusses fabrication strategies employed in the development of 4D living materials and examples of their 4D activities driven by cellular events or processes. The fabrication strategies investigated throughout are focused on those that facilitate the responsive, functional transformation of the 3D structure with the time that extends beyond changes driven solely by material features, and enable an increase in the cell-dependent functional capacity of the construct. Living materials comprised of cells and their own products, as well as both cells and added non-living materials are investigated. Further, the authors analyze how material complexities and biological complexities have thus far been interfaced to allow for intricate living behaviors, and discuss the design considerations and challenges encountered in developing living materials. Finally, the future directions of living materials are outlined.

show abstract

“…The fabrication of well-defined ELM microparticles on a large scale requires new fabrication methods; at the same time, the fabrication techniques need to be compatible with biological cells. For instance, microfluidic fabrication was used to generated hydrogel microparticles with genetically engineered lactam-responsive E. coli encapsulated inside (Ma et al, 2019), and the microparticles can be used for detection of lactam species in a dose-dependent manner. Electrostatic extrusion was used for the fabrication of bacteria loaded alginate-methacrylate hydrogel beads (Li et al, 2017), the encapsulated reporter bacteria responded with a strong fluorescence signal when exposed to the autoinducer molecules.…”

Section: Elms-based Structures For Biosensingmentioning

confidence: 99%

Engineered Living Materials-Based Sensing and Actuation

Liu

2020

Front. Sens.

View full text Add to dashboard Cite

The integration of functional synthetic materials and living biological entities has emerged as a new and powerful approach to create adaptive and functional structures with unprecedented performance and functionalities. Such hybrid structures are also called engineered living materials (ELMs). ELMs have the potential to realize many highly-desired properties, which are usually only found in biological systems, such as the abilities to self-power, self-heal, response to biosignals, and self-sustainable. Motivated by that, in recent years, researchers have started to explore the use of ELMs in many areas, among them, sensing and actuation is the area that has seen the most progress. In this short review, we briefly reviewed the important recent development in ELMs-based sensors and actuators, with a focus on their materials and structural design, new fabrication technologies, and bio-related applications. Current challenges and future directions in this field are also identified to help with future development in this emerging interdisciplinary field.

show abstract

Hydrogel Microparticles Functionalized with Engineered Escherichia coli as Living Lactam Biosensors

Cited by 12 publications

References 34 publications

Biological and technical challenges for implementation of yeast‐based biosensors

Biological and technical challenges for implementation of yeast‐based biosensors

Hylozoic by Design: Converging Material and Biological Complexities for Cell‐Driven Living Materials with 4D Behaviors

Engineered Living Materials-Based Sensing and Actuation

Contact Info

Product

Resources

About