Hydrogel screening approaches for bone and cartilage tissue regeneration

Benmassaoud, Mohammed Mehdi; Gultian, Kirstene A.; DiCerbo, Matthew; Vega, Sebastián L.

doi:10.1111/nyas.14247

Cited by 25 publications

(18 citation statements)

References 125 publications

(148 reference statements)

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“…Hydrogels mimic the physical and chemical conditions of the extracellular matrix, promoting cell differentiation and multiplication along with bolstering integration with trauma sites (Arakaki et al, 2010). Combinatorial hydrogels are a class of hydrogels that allow for the study of cellular responses to multiple biophysical (e.g., crosslink density) and biochemical (e.g., ligand tethering) signals (Benmassaoud et al, 2020).…”

Section: Hydrogels (Hg)mentioning

confidence: 99%

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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Cartilage, especially articular cartilage, is a unique connective tissue consisting of chondrocytes and cartilage matrix that covers the surface of joints. It plays a critical role in maintaining joint durability and mobility by providing nearly frictionless articulation for mechanical load transmission between joints. Damage to the articular cartilage frequently results from sport-related injuries, systemic diseases, degeneration, trauma, or tumors. Failure to treat impaired cartilage may lead to osteoarthritis, affecting more than 25% of the adult population globally. Articular cartilage has a very low intrinsic self-repair capacity due to the limited proliferative ability of adult chondrocytes, lack of vascularization and innervation, slow matrix turnover, and low supply of progenitor cells. Furthermore, articular chondrocytes are encapsulated in low-nutrient, low-oxygen environment. While cartilage restoration techniques such as osteochondral transplantation, autologous chondrocyte implantation (ACI), and microfracture have been used to repair certain cartilage defects, the clinical outcomes are often mixed and undesirable. Cartilage tissue engineering (CTE) may hold promise to facilitate cartilage repair. Ideally, the prerequisites for successful CTE should include the use of effective chondrogenic factors, an ample supply of chondrogenic progenitors, and the employment of cell-friendly, biocompatible scaffold materials. Significant progress has been made on the above three fronts in past decade, which has been further facilitated by the advent of 3D bio-printing. In this review, we briefly discuss potential sources of chondrogenic progenitors. We then primarily focus on currently available chondrocyte-friendly scaffold materials, along with 3D bioprinting techniques, for their potential roles in effective CTE. It is hoped that this review will serve as a primer to bring cartilage biologists, synthetic chemists, biomechanical engineers, and 3D-bioprinting technologists together to expedite CTE process for eventual clinical applications.

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Section: Hydrogels (Hg)mentioning

confidence: 99%

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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“…The present work was concerned with the development of a valid rabbit model for the induction of mandibular OM based on controlled intra‐dental placement of arsenic trioxide paste followed by efficient treatment trials via injectable biomimetic hydrogels. The biomimetic injectable hydrogels attracted attention because of their ease of administration, drug delivery capability, structural similarity to the native extracellular matrix, and their excellent biological response 33‐35 . Biodegradability of biomimetic hydrogels precludes the need for a secondary surgery with the associated risk of infection.…”

Section: Discussionmentioning

confidence: 99%

Local treatment of experimental mandibular osteomyelitis with an injectable biomimetic gentamicin hydrogel using a new rabbit model

et al. 2021

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Mandibular osteomyelitis (OM) is a challenging disease. Our objective was to assess a new OM model in rabbits induced by arsenic trioxide and to assess the efficacy of local treatment of OM using injectable gentamicin‐collagen hydrogels (GNT‐COLL). OM was induced unilaterally by controlled confinement of arsenic trioxide paste to the root canal of lower incisors of rabbits, while OM progression was characterized for 16 weeks. On the other hand, two injectable COLL hydrogels functionalized with GNT were prepared and characterized for physicochemical properties; a simple GNT‐COLL and a nanohydroxyapatite (nHA)‐ loaded hydrogel (GNT‐COLL/nHA). The two hydrogels were evaluated to treat OM model, while a multidose intramuscular GNT solution served as positive control. Outcomes were assessed by standard methods at 4 and 12 weeks post‐surgery. The clinical, radiographical, and histopathological findings provided evidence for the validity of the arsenic‐induced OM. The results demonstrated that a single intra‐lesional injection of the two hydrogels was more suppressive to OM compared to multidose systemic GNT. The composite GNT‐COLL/nHA hydrogel proved to induce early preservation of alveolar bone (ridge) length and higher amount of bone area\total area at 4 weeks (40.53% ± 2.34) followed by GNT‐COLL (32.21% ± 0.72). On the other hand, the positive control group revealed the least ridge length and bone area\total area (26.22% ± 1.32) at 4 weeks. Both hydrogels successfully arrested OM with no signs of recurrence for up to 12 weeks. Therefore, results support the greater advantages of the composite hydrogel as an osteogenic/antibiotic delivery system in OM treatment.

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“…Among the hydrogel scaffolds, alginate and agarose are inert, presenting no intrinsic adherent molecules, and provide reduced adherence to hydrogels such as collagen and matrigel (Awad, Wickham, Leddy, Gimble, & Guilak, 2004). Increasing evidence has focused on the control of material properties of these systems by changing the chemical components, which simultaneously varied ECM adhesive ability or mechanics, making them become possible to regulate cell behavior and meet the requirement of cartilage repair (Benmassaoud, Gultian, DiCerbo, & Vega, 2019). Compared with bare chondroitin sulfate (CS), mussel-inspired tissue adhesive hydrogel based on polydopamine-CS complex was more effective in exerting its functions on adhered cells to upregulate chondrogenic differentiation (Han et al, 2018).…”

Section: Extracellular Matrix Adhesion Of the Microenvironment Can DImentioning

confidence: 99%

Effect of matrix stiffness and adhesion ligand density on chondrogenic differentiation of mesenchymal stem cells

Zhan

2019

J Biomedical Materials Res

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Adhesion ligands and mechanical properties of extracellular matrix (ECM) play significant roles in directing mesenchymal stem cells' (MSCs) behaviors, but how they affect chondrogenic differentiation of MSCs has rarely been studied. In this study, we investigated the effects of matrix stiffness and adhesion ligand density on proliferation and chondrogenic differentiation of MSCs by using UV crosslinked hydrogels comprised of methacrylated gelatin (GelMA) and poly(ethylene glycol) diacrylate (PEGDA) of different weight ratios. The PEGDA/GelMA hydrogels were fabricated by adjusting the weight ratio of PEGDA and GelMA with low or high adhesion ligand density (0.05 and 0.5% GelMA, respectively) and independent tunable stiffness (1.6, 6, and 25 kPa separately for hydrogels with 5, 10, and 15% PEGDA). MSCs presented differential behaviors to ECM by adjusting its adhesion ligand density and stiffness. Cell proliferation and chondrogenic differentiation could be enhanced with the improvement of adhesive properties and stiffness, evidenced by cell viability assay, hematoxylin-eosin staining, Safranin O staining, immunohistochemistry (Collagen types II, Col2a1), as well as the chondrogenic genes expression of Col2a1, Acan, and Sox9. This study may provide new strategies to design the scaffolds for cartilage tissue engineering. K E Y W O R D S adhesion, chondrogenic differentiation, extracellular matrix (ECM), mesenchymal stem cell (MSCs), stiffness

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Hydrogel screening approaches for bone and cartilage tissue regeneration

Cited by 25 publications

References 125 publications

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Local treatment of experimental mandibular osteomyelitis with an injectable biomimetic gentamicin hydrogel using a new rabbit model

Effect of matrix stiffness and adhesion ligand density on chondrogenic differentiation of mesenchymal stem cells

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