Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment

Belousov, Andrei S.; Titov, Sergei; Shved, Nikita A.; Malykin, Grigorii V.; Kovalev, Vladimir A.; Супрунова, Ірина Валеріївна; Kumeiko, Vadim

doi:10.1016/bs.irn.2020.03.025

Cited by 10 publications

(7 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The possibility of modulating the behavior of glioblastoma cells using hydrogels based on modified pectins and extracellular matrix proteins has already been shown in our previous article [ 8 ]. In this work, we have elaborated pectin films and NPs for prospective anticancer therapy.…”

Section: Discussionmentioning

confidence: 99%

“…Nanomechanical properties of pectin films and NPs are crucial for the effect on tumor cells, which was demonstrated by the example of hydrogels in our recent work [ 8 ]. The films obtained were found to be quite soft.…”

Section: Discussionmentioning

confidence: 99%

“…The main advantages of carbohydrate polymers are low toxicity, biodegradability, biocompatibility and minimal immunogenicity. These features, combined with the ease of chemical modification and the low cost of raw materials, allow for the large-scale production of finely tuned therapeutic systems [ 8 , 9 , 10 ]. Carbohydrates are used to create various forms of materials, such as particles, films and hydrogels [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…At the same time, pectin-based biomaterials could mimic the animal polysaccharides attributed to the components of ECM. Pectin helps to create a supportive microenvironment for neural cells, modulate cell migration and affect tissue-repair processes [ 8 ]. Moreover, chemical modifications of naturally produced pectin or improvements in the functional supplements give great opportunities for the development of pectin-based biomaterials targeted to diversified biomedical applications [ 8 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Preparation and Characterization of Hydrogel Films and Nanoparticles Based on Low-Esterified Pectin for Anticancer Applications

Patlay,

Belousov,

Silant’ev

et al. 2023

Polymers

View full text Add to dashboard Cite

Prospective adjuvant anticancer therapy development includes the establishing of drug delivery systems based on biocompatible and biodegradable carriers. We have designed films and nanoparticles (NPs) based on low-esterified pectin hydrogel using the ionic gelation method. We investigated morphology, nanomechanical properties, biocompatibility and anticancer activity. Hydrogel films are characterized by tunable viscoelastic properties and surface nanoarchitectonics through pectin concentration and esterification degree (DE), expressed in variable pore frequency and diameter. An in vitro study showed a significant reduction in metabolic activity and the proliferation of the U87MG human glioblastoma cell line, probably affected via the adhesion mechanism. Glioma cells formed neurosphere-like conglomerates with a small number of neurites when cultured on fully de-esterified pectin films and they did not produce neurites on the films prepared on 50% esterified pectin. Pectin NPs were examined in terms of size distribution and nanomechanical properties. The NPs’ shapes were proved spherical with a mean diameter varying in the range of 90–115 nm, and a negative zeta potential from −8.30 to −7.86 mV, which indicated their stability. The NPs did not demonstrate toxic effect on cells or metabolism inhibition, indicating good biocompatibility. Nanostructured biomaterials prepared on low-esterified pectins could be of interest for biomedical applications in adjuvant anticancer therapy and for designing drug delivery systems.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation and Characterization of Hydrogel Films and Nanoparticles Based on Low-Esterified Pectin for Anticancer Applications

Patlay,

Belousov,

Silant’ev

et al. 2023

Polymers

View full text Add to dashboard Cite

show abstract

“…in the number of interaction sites) limits the mobility of collagen molecules, leading to the formation of collagen fibrils in the ordered form of precipitates. The mixing in neutral conditions of alkaline de-esterified pectin/CaCl2 with different rations of collagen type I and/or IV (and the subsequent incubation at 37°C) was reported to create an optimal microenvironment for glioblastoma treatment ( Belousov et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics

Merli

Sardelli

Baranzini

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue. Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen). Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments. By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition. In addition, the results indicate that not only cells survive and proliferate following 3D-bioprinting, but they can also interact and reorganize hydrogel microstructure. Taken together, the results suggest that pectin-based hydrogels could be successfully applied for neural cell culture.

show abstract

Polysaccharide-based electroconductive hydrogels: Structure, properties and biomedical applications

Yazdi

Zarrintaj

Khodadadi

et al. 2022

Carbohydrate Polymers

View full text Add to dashboard Cite

Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment

Cited by 10 publications

References 51 publications

Preparation and Characterization of Hydrogel Films and Nanoparticles Based on Low-Esterified Pectin for Anticancer Applications

Preparation and Characterization of Hydrogel Films and Nanoparticles Based on Low-Esterified Pectin for Anticancer Applications

Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics

Polysaccharide-based electroconductive hydrogels: Structure, properties and biomedical applications

Contact Info

Product

Resources

About