Hydrogels composed of hyaluronic acid and dendritic ELPs: hierarchical structure and physical properties

Shmidov, Yulia; Zhou, Mingjun; Yosefi, Gal; Bitton, Ronit; Matson, John B.

doi:10.1039/c8sm02450b

Cited by 25 publications

(28 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The particle size measurement analyses indicated that the coacervation of AADAAC-F4 might follow a stepwise process in which sub-micron aggregates grow into micrometer-sized coacervates. Such a stepwise process is similar to that of the dendritic thermoresponsive ELP 55 and some strong coacervatable short ELPs that we reported earlier 49 – 51 . In addition, DLS measurements were carried out in the presence of Cd 2+ (Fig.…”

Section: Resultssupporting

confidence: 82%

Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence

Sumiyoshi

Suyama

Tatsubo

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The development of simple and safe methods for recovering environmental pollutants, such as heavy metals, is needed for sustainable environmental management. Short elastin-like peptide (ELP) analogues conjugated with metal chelating agents are considered to be useful as metal sequestering agents as they are readily produced, environment friendly, and the metal binding domain can be selected based on any target metal of interest. Due to the temperature dependent self-assembly of ELP, the peptide-based sequestering agents can be transformed from the solution state into the particles that chelate metal ions, which can then be collected as precipitates. In this study, we developed a peptide-based sequestering agent, AADAAC-(FPGVG)4, by introducing the metal-binding sequence AADAAC on the N-terminus of a short ELP, (FPGVG)4. In turbidity measurements, AADAAC-(FPGVG)4 revealed strong self-assembling ability in the presence of metal ions such as Cd2+ and Zn2+. The results from colorimetric analysis indicated that AADAAC-(FPGVG)4 could capture Cd2+ and Zn2+. Furthermore, AADAAC-(FPGVG)4 that bound to metal ions could be readily recycled by treatment with acidic solution without compromising its metal binding affinity. The present study indicates that the fusion of the metal-binding sequence and ELP is a useful and powerful strategy to develop cost-effective heavy metal scavenging agents with low environmental impacts.

show abstract

Section: Resultssupporting

confidence: 82%

Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence

Sumiyoshi

Suyama

Tatsubo

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…It also has a large viscosity and is viscous. When the shear rate increases, the scattered chains are rolled and contracted into a group, thereby reducing mutual hooking and the apparent viscosity, which leads to the phenomenon of shear thinning (30). There was no significant difference in the rheology of the gel at room temperature (25°C) versus normal skin surface temperature in vivo (32°C).…”

Section: Resultsmentioning

confidence: 92%

Transcutol® P/Cremophor® EL/Ethyl Oleate–Formulated Microemulsion Loaded into Hyaluronic Acid–Based Hydrogel for Improved Transdermal Delivery and Biosafety of Ibuprofen

Zhang

Wang

et al. 2019

AAPS PharmSciTech

View full text Add to dashboard Cite

In the present study, a novel transdermal delivery system was developed and its advantages were demonstrated. Ibuprofen is a commonly used anti-inflammatory, antipyretic, and analgesic drug; however, because of its short biological half-life, it must be frequently administered orally and is highly irritating to the digestive tract. To prepare a novel transdermal delivery system for ibuprofen, a microemulsion was used as a drug carrier and dispersed in a hyaluronic acid-based hydrogel (ME/Gel) to increase percutaneous drug absorption while avoiding gastrointestinal tract irritation. The prepared microemulsion had a droplet size of ~90 nm, and the microemulsion had good stability in the hydrogel. Rheological tests revealed that the ME/Gel is a pseudoplastic fluid with decreased viscosity and increased shear rate. It displayed a certain viscoelasticity, and the microemulsion distribution displayed minor effects on the rheological characteristics of the hydrogel system. There was no significant difference in the rheology of the ME/Gel at 25°C and 32°C (normal skin surface temperature), which is beneficial for clinical application. Drug transdermal flux was significantly higher than that of the hydrogel and commercial cream groups (p < 0.01). The 24-h cumulative drug permeation amount was 1.42-fold and 2.52-fold higher than that of the hydrogel and cream groups, respectively. By loading into the ME/Gel, the cytotoxicity of the drug to HaCaT cells was reduced. These results indicate that the prepared ME/Gel can effectively improve transdermal ibuprofen delivery and the biosafety of the drug and could therefore have applicability as a drug delivery system.

show abstract

“…VEGF molecules present in the medium during gelation, may or may not be captured in the gel, depending on the interactions between the VEGF molecules and the modified alginate polymer chains. 39 In Fig. 6A it can be seen that Alg-Hep, Alg-RGD-Hep and Alg-RGD + Alg-Hep gels captured the highest amounts of VEGF.…”

Section: Kinetics Of Vascular Endothelial Growth Factor (Vegf) Releasementioning

confidence: 91%

Effect of heparin and peptide conjugation on structure and functional properties of alginate in solutions and hydrogels

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Hydrogels composed of hyaluronic acid and dendritic ELPs: hierarchical structure and physical properties

Abstract: Hydrogels that mimic the native extracellular matrix were prepared from hyaluronic acid (HA) and amine-terminated dendritic elastin-like peptides (denELPs) of generations 1, 2, and 3 (G1, 2, and 3) as crosslinking units.

Cited by 25 publications

References 41 publications

Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence

Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence

Transcutol® P/Cremophor® EL/Ethyl Oleate–Formulated Microemulsion Loaded into Hyaluronic Acid–Based Hydrogel for Improved Transdermal Delivery and Biosafety of Ibuprofen

Effect of heparin and peptide conjugation on structure and functional properties of alginate in solutions and hydrogels

Contact Info

Product

Resources

About