Hydrogels for the Detection and Management of Protease Levels

Patrick, Alison; Ulijn, Rein V.

doi:10.1002/mabi.200900457

Cited by 25 publications

(15 citation statements)

References 48 publications

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“…Elastase-sensitive peptides (e.g., AAAAAAA, AAPV and AAPVRGGG) [201–204] and chymotrypsin-sensitive peptides (e.g., GGYRG) [205] have been used for proteolytic modification of PEG hydrogels. Short peptide sequences, such as GL, GFL and GFGL, have also been functionalized with dimethacrylate for crosslinking HEMA or HEMA/PEGMA to make papain-sensitive hydrogels [206].…”

Section: Design and Synthesis Of Ecm-mimetic Hydrogel Scaffoldsmentioning

confidence: 99%

Design properties of hydrogel tissue-engineering scaffolds

Zhu

Marchant

2011

Expert Review of Medical Devices

1,234

910

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This article summarizes the recent progress in the design and synthesis of hydrogels as tissue-engineering scaffolds. Hydrogels are attractive scaffolding materials owing to their highly swollen network structure, ability to encapsulate cells and bioactive molecules, and efficient mass transfer. Various polymers, including natural, synthetic and natural/synthetic hybrid polymers, have been used to make hydrogels via chemical or physical crosslinking. Recently, bioactive synthetic hydrogels have emerged as promising scaffolds because they can provide molecularly tailored biofunctions and adjustable mechanical properties, as well as an extracellular matrix-like microenvironment for cell growth and tissue formation. This article addresses various strategies that have been explored to design synthetic hydrogels with extracellular matrix-mimetic bioactive properties, such as cell adhesion, proteolytic degradation and growth factor-binding.

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Section: Design and Synthesis Of Ecm-mimetic Hydrogel Scaffoldsmentioning

confidence: 99%

Design properties of hydrogel tissue-engineering scaffolds

Zhu

Marchant

2011

Expert Review of Medical Devices

1,234

910

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“…Hydrogels decorated with biological ligands or enzyme cleavable groups have promise not only as cell culture scaffolds, 5 but also as implantable biomaterials 6 or protease sensors. 7 In recent examples of the latter, PEGA hydrogels functionalised with cleavable peptide sequences were developed as drug delivery platforms 8 whereas self-assembled peptide gels can respond to enzymatic cleavage by undergoing a sol-to-gel transition. 9 Furthermore, incorporating recognition and/or sensing elements within a three-dimensional cell culture material that can be formed around cells, yet not be degraded by them, would support cell growth and allow the behaviour of these proliferating cells to be non-invasively monitored.…”

Section: Introductionmentioning

confidence: 99%

A modular self-assembly approach to functionalised β-sheet peptide hydrogel biomaterials

et al. 2016

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Two complementary β-sheet-forming decapeptides have been created that form binary self-repairing hydrogels upon combination of the respective free-flowing peptide solutions at pH 7 and >0.28 wt%. The component peptides showed little structure separately but formed extended β-sheet fibres upon mixing, which became entangled to produce stiff hydrogels. Microscopy revealed two major structures; thin fibrils with a twisted or helical appearance and with widths comparable to the predicted lengths of the peptides within a β-sheet, and thicker, longer, interwoven fibres that appear to comprise laterally-packed fibrils. A range of gel stiffnesses (G' from 0.05 to 100 kPa) could be attained in this system by altering the assembly conditions, stiffnesses that cover the rheological properties desirable for cell culture scaffolds. Doping in a RGD-tagged component peptide at 5 mol% improved 3T3 fibroblast attachment and viability compared to hydrogel fibres without RGD functionalisation.

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“…These proteases at elevated concentrations are biomarkers for chronic wound treatment with protease sequestrant dressings [35,36,37]. Therefore, the use of nanocellulosic aerogels (NA) as the transducer surface attached to a fluorescent peptide substrate, such as succinyl-alanine-proline-alanine-4-amino-7-methyl-coumarin (Suc-Ala-Pro-Ala-AMC) or succinyl-alanine-alanine-proline-valine-4-amino-7-methyl-coumarin (Suc-Ala-Ala-Pro-Val-AMC) [38], which has selectivity for HNE, not only offers specificity, but also offers a way to detect HNE in chronic wound fluid. The fluorescent tripeptide-substrate (Suc-Ala-Pro-Ala-AMC) was tethered to a NA by (1) esterification of cellulose C6 surface hydroxyl groups with glycidyl-fluorenylmethyloxycarbonyl (FMOC), (2) deprotection and (3) coupling of the immobilized glycine with the tripeptide (peptide-nanocellulosic aerogel (PepNA)).…”

Section: Introductionmentioning

confidence: 99%

Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton

Edwards

Fontenot

Prevost

et al. 2016

Sensors

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Nanocellulosic aerogels (NA) provide a lightweight biocompatible material with structural properties, like interconnected high porosity and specific surface area, suitable for biosensor design. We report here the preparation, characterization and activity of peptide-nanocellulose aerogels (PepNA) made from unprocessed cotton and designed with protease detection activity. Low-density cellulosic aerogels were prepared from greige cotton by employing calcium thiocyanate octahydrate/lithium chloride as a direct cellulose dissolving medium. Subsequent casting, coagulation, solvent exchange and supercritical carbon dioxide drying afforded homogeneous cellulose II aerogels of fibrous morphology. The cotton-based aerogel had a porosity of 99% largely dominated by mesopores (2–50 nm) and an internal surface of 163 m2·g−1. A fluorescent tripeptide-substrate (succinyl-alanine-proline-alanine-4-amino-7-methyl-coumarin) was tethered to NA by (1) esterification of cellulose C6 surface hydroxyl groups with glycidyl-fluorenylmethyloxycarbonyl (FMOC), (2) deprotection and (3) coupling of the immobilized glycine with the tripeptide. Characterization of the NA and PepNA included techniques, such as elemental analysis, mass spectral analysis, attenuated total reflectance infrared imaging, nitrogen adsorption, scanning electron microscopy and bioactivity studies. The degree of substitution of the peptide analog attached to the anhydroglucose units of PepNA was 0.015. The findings from mass spectral analysis and attenuated total reflectance infrared imaging indicated that the peptide substrate was immobilized on to the surface of the NA. Nitrogen adsorption revealed a high specific surface area and a highly porous system, which supports the open porous structure observed from scanning electron microscopy images. Bioactivity studies of PepNA revealed a detection sensitivity of 0.13 units/milliliter for human neutrophil elastase, a diagnostic biomarker for inflammatory diseases. The physical properties of the aerogel are suitable for interfacing with an intelligent protease sequestrant wound dressing.

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Hydrogels for the Detection and Management of Protease Levels

Cited by 25 publications

References 48 publications

Design properties of hydrogel tissue-engineering scaffolds

Design properties of hydrogel tissue-engineering scaffolds

A modular self-assembly approach to functionalised β-sheet peptide hydrogel biomaterials

Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton

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