Hydrogels for tissue engineering: scaffold design variables and applications

Drury, Jeanie L.; Mooney, David J.

doi:10.1016/s0142-9612(03)00340-5

Cited by 4,557 publications

(3,501 citation statements)

References 138 publications

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“…56 Hyaluronic acid, a simple glucosaminoglycan which is found in most mammalian tissues, is especially prevalent in wounds during the healing process, is known to promote the healing of damaged skin, and could be used with microbial cellulose in order to create an even more effective wound dressing material. 59 Indeed, scientists have already begun to investigate the healing potential of augmented microbial cellulose.…”

Section: Microbial Cellulose As a Wound-healing Systemmentioning

confidence: 99%

The Future Prospects of Microbial Cellulose in Biomedical Applications

et al. 2006

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Microbial cellulose has proven to be a remarkably versatile biomaterial and can be used in wide variety of applied scientific endeavors, such as paper products, electronics, acoustics, and biomedical devices. In fact, biomedical devices recently have gained a significant amount of attention because of an increased interest in tissue-engineered products for both wound care and the regeneration of damaged or diseased organs. Due to its unique nanostructure and properties, microbial cellulose is a natural candidate for numerous medical and tissue-engineered applications. For example, a microbial cellulose membrane has been successfully used as a wound-healing device for severely damaged skin and as a small-diameter blood vessel replacement. The nonwoven ribbons of microbial cellulose microfibrils closely resemble the structure of native extracellullar matrices, suggesting that it could function as a scaffold for the production of many tissue-engineered constructs. In addition, microbial cellulose membranes, having a unique nanostructure, could have many other uses in wound healing and regenerative medicine, such as guided tissue regeneration (GTR), periodontal treatments, or as a replacement for dura mater (a membrane that surrounds brain tissue). In effect, microbial cellulose could function as a scaffold material for the regeneration of a wide variety of tissues, showing that it could eventually become an excellent platform technology for medicine. If microbial cellulose can be successfully mass produced, it will eventually become a vital biomaterial and will be used in the creation of a wide variety of medical devices and consumer products.

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Section: Microbial Cellulose As a Wound-healing Systemmentioning

confidence: 99%

The Future Prospects of Microbial Cellulose in Biomedical Applications

et al. 2006

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“…1 Due to their unique properties such as high porosity, permeability for gases, nutrients and metabolites, as well as their compatibility with physiological conditions, hydrogels have been extensively studied as material support for immobilizing cells, cultivating cells in 3D, 2 biomedical applications, drug delivery and tissue engineering. 3 Two distinct approaches namely "top-down" or "bottom-up" have recently emerged in hydrogel engineering. Top-down approaches control the microscale features (i.e.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

et al. 2016

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We report a method to rapidly fabricate alginate hydrogel particles of specific sizes and shapes.Our method is based on the formation of arrays of droplets of pre-hydrogel solutions on superhydrophobic-hydrophilic patterns using the process of discontinuous dewetting, followed by their gelation via the parallel addition of CaCl 2 to the individual droplets via the sandwiching method. We demonstrate that viability of living cells incorporated within the hydrogel particles is higher during the long-term cultivation than in the case of cells cultured in the bulk threedimensional hydrogel matrix. Incorporation of magnetic particles into the free-standing hydrogel particles containing living cells enabled ease manipulation of the particles using an external magnetic field.

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“…Consequently, there is currently a focus on the encapsulation of cells in hydrogels [19,20]. The highly hydrated structure of hydrogels allows for good mass transport properties [21] so that nutrients, waste products, and signalling molecules can effectively perfuse the scaffold to maintain cell viability and thereby facilitate paracrine interactions.…”

Section: Introductionmentioning

confidence: 99%

An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co‐culture

Hunt

Shelton

Grover

2009

Biotechnology Journal

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International audienceThere is significant interest in the development of tissue engineered skin analogues which replace both the dermal and the epidermal layer, without the use of animal or human derived products such as collagen or de-epidermalised dermis (DED). In this study, we proposed that alginate hydrogel could be used to encapsulate fibroblasts and that keratinocytes could be cultured on the surface to form a bilayered structure which could be used to deliver the co-culture to a wound bed, initially providing wound closure and eventually expediting the healing process. Encapsulation of fibroblasts in 5% alginate hydrogel effectively inhibited their proliferation, whilst maintaining cell viability allowing keratinocytes to grow uninhibited by fibroblast overgrowth to produce a stratified epidermal layer. It was shown that the alginate degradation process was not influenced by the presence of fibroblasts within the hydrogel and that lowering the alginate concentration to 2% w/v increased the rate of degradation. Cells released from the scaffold were able to secrete extra-cellular matrix (ECM) and thus should replace the degrading scaffold with normal ECM following application to the wound site. These findings demonstrate that alginate hydrogel may be an effective delivery vehicle and scaffold for the healing of full-thickness skin wounds

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Hydrogels for tissue engineering: scaffold design variables and applications

Cited by 4,557 publications

References 138 publications

The Future Prospects of Microbial Cellulose in Biomedical Applications

The Future Prospects of Microbial Cellulose in Biomedical Applications

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co‐culture

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