Hydrogen enhances adaptation of rice seedlings to cold stress via the reestablishment of redox homeostasis mediated by miRNA expression

Xu, Sheng; Jiang, Yue; Cui, Weiti; Jin, Qijiang; Zhang, Yihua; Bu, Duo; Fu, Jiangyan; Ren, Wang; Zhou, Feng; Shen, Wenbiao

doi:10.1007/s11104-016-3106-8

Cited by 46 publications

(29 citation statements)

References 75 publications

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“…thaliana , chilling resistance could occurs via negatively regulates miR398 expression and the expressions of AtCSD1 and AtCSD2 was induced accordingly [76]; in Triticum aestivum , the expression of miR398 was inhibited by cold stress and CSD gene was up-regulated synchronously [77]; in O . sativa , the expressions of miR398 and its targets ( OsCSD1 and OsCSD2 ) were inhibited and increased under cold stress, respectively [78]. In the present study, we perform the modification 5’ RLM-RACE experiment to verified the putative regulatory miRNAs of CsSODs .…”

Section: Discussionmentioning

confidence: 91%

Genome-wide investigation of superoxide dismutase (SOD) gene family and their regulatory miRNAs reveal the involvement in abiotic stress and hormone response in tea plant (Camellia sinensis)

Zhou

Zhu

et al. 2019

PLoS ONE

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Superoxide dismutases (SODs), as a family of metalloenzymes related to the removal of reactive oxygen species (ROS), have not previously been investigated at genome-wide level in tea plant. In this study, 10 CsSOD genes were identified in tea plant genome, including 7 Cu/Zn-SODs (CSDs), 2 Fe-SODs (FSDs) and one Mn-SOD (MSD), and phylogenetically classified in three subgroups, respectively. Physico-chemical characteristic, conserved motifs and potential protein interaction analyses about CsSOD proteins were carried out. Exon-intron structures and codon usage bias about CsSOD genes were also examined. Exon-intron structures analysis revealed that different CsSOD genes contained various number of introns. On the basis of the prediction of regulatory miRNAs of CsSODs, a modification 5’ RNA ligase-mediated (RLM)-RACE was performed and validated that csn-miR398a-3p-1 directly cleaves CsCSD4. By prediction of cis-acting elements, the expression patterns of 10 CsSOD genes and their regulatory miRNAs were detected under cold, drought, exogenous methyl jasmonate (MeJA) and gibberellin (GA3) treatments. The results showed that most of CsSODs except for CsFSD2 were induced under cold stress and CsCSDs may play primary roles under drought stress; exogenous GA3 and MeJA could also stimulated/inhibited distinct CsSODs at different stages. In addition, we found that csn-miR398a-3p-1 negatively regulated the expression of CsCSD4 may be a crucial regulatory mechanism under cold stress. This study provides a certain basis for the studies about stress resistance in tea plants, even provide insight into comprehending the classification, evolution, diverse functions and influencing factors of expression patterns for CsSOD genes.

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Section: Discussionmentioning

confidence: 91%

Genome-wide investigation of superoxide dismutase (SOD) gene family and their regulatory miRNAs reveal the involvement in abiotic stress and hormone response in tea plant (Camellia sinensis)

Zhou

Zhu

et al. 2019

PLoS ONE

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“…U6 snRNA was used as internal control. The rest steps were the same as the approaches described previously [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

Hydrogen peroxide is involved in hydrogen sulfide-induced lateral root formation in tomato seedlings

et al. 2017

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BackgroundBoth hydrogen sulfide (H2S) and hydrogen peroxide (H2O2) are separately regarded as a highly reactive molecule involved in root morphogenesis. In this report, corresponding causal link governing lateral root formation was investigated.MethodsBy using pharmacological, anatomic, and molecular approaches, evidence presented here revealed the molecular mechanism underlying tomato lateral root development triggered by H2S.ResultsA H2S donor sodium hydrosulfide (NaHS) triggered the accumulation of H2O2, the up-regulation of RBOH1 transcript, and thereafter tomato lateral root formation. Above responses were sensitive to the H2O2 scavenger (dimethylthiourea; DMTU) and the inhibitor of NADPH oxidase (diphenylene idonium; DPI), showing that the accumulations of H2O2 and increased RBOH1 transcript were respectively prevented. Lateral root primordial and lateral root formation were also impaired. Further molecular evidence revealed that H2S-modulated gene expression of cell cycle regulatory genes, including up-regulation of SlCYCA2;1, SlCYCA3;1, and SlCDKA1, and the down-regulation of SlKRP2, were prevented by the co-treatment with DMTU or DPI. Above mentioned inducing phenotypes were consistent with the changes of lateral root formation-related microRNA transcripts: up-regulation of miR390a and miR160, and with the opposite tendencies of their target genes (encoding auxin response factors). Contrasting tendencies were observed when DMTU or DPI was added together. The occurrence of H2S-mediated S-sulfhydration during above responses was preliminarily discovered.ConclusionsOverall, these results suggested an important role of RBOH1-mediated H2O2 in H2S-elicited tomato lateral root development, and corresponding H2S-target proteins regulated at transcriptional and post-translational levels.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1110-7) contains supplementary material, which is available to authorized users.

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“…At present, the attention on the biological roles of H 2 has progressed from animals to plants (C. Li et al, 2018; Zeng et al, 2013). Ample evidence has confirmed that, in plants, exogenous H 2 plays multiple roles to aid in protecting against ultraviolet‐induced oxidative damage (Xie et al, 2015); in enhancing abiotic tolerance to cold, drought and salinity (Su et al, 2018; Xie et al, 2014; Xie, Mao, Lai, Zhang, & Shen, 2012; S. Xu et al, 2017); in alleviating damage upon exposure to heavy metals (Wu et al, 2020); in improving tolerance to paraquat (Jin et al, 2013) and in ameliorating the effect of light stress (X. Zhang, Zhao, Wang, Shen, & Xu, 2015). It has been shown that H 2 can be positively involved in the regulation of plant growth and development, such as seed germination (S. Xu et al, 2013), root morphogenesis (Cao et al, 2017) and fresh‐keeping of fruits and cut flowers (Hu, Zhao, Li, & Shen, 2018; Su et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Molecular hydrogen–induced salinity tolerance requires melatonin signalling inArabidopsis thaliana

Yang

Shao

et al. 2020

Plant Cell & Environment

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Melatonin (MT) plays positive roles in salinity stress tolerance. However, the upstream signalling components that regulate MT are poorly understood. Here, we report that endogenous MT acts downstream of molecular hydrogen (H2) in the salinity response in Arabidopsis. The addition of hydrogen‐rich water and expression of the hydrogenase1 gene (CrHYD1) from Chlamydomonas reinhardtii increased endogenous H2 and MT levels and enhanced salinity tolerance. These results were not observed in the absence of serotonin N‐acetyltransferase gene (SNAT). H2 increased the levels of SNAT transcripts in the wild‐type and CrHYD1 lines, which had lower Na+/K+ ratios and higher levels of ion transport–related gene transcripts. These changes were not observed in atsnat/CrHYD1‐4 hybrids. The increased MT‐dependent Na+ extrusion observed in the CrHYD1 plants resulted, at least in part, from enhanced Na+/H+ antiport across the plasma membrane. The endogenous H2‐induced MT‐dependent regulation of ion and redox homeostasis was impaired in the atsnat/CrHYD1‐4 hybrids. Taken together, these results demonstrate that MT‐induced salinity tolerance is induced by a H2 signalling cascade that regulates ion and redox homeostasis in response to salinity.

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Hydrogen enhances adaptation of rice seedlings to cold stress via the reestablishment of redox homeostasis mediated by miRNA expression

Cited by 46 publications

References 75 publications

Genome-wide investigation of superoxide dismutase (SOD) gene family and their regulatory miRNAs reveal the involvement in abiotic stress and hormone response in tea plant (Camellia sinensis)

Genome-wide investigation of superoxide dismutase (SOD) gene family and their regulatory miRNAs reveal the involvement in abiotic stress and hormone response in tea plant (Camellia sinensis)

Hydrogen peroxide is involved in hydrogen sulfide-induced lateral root formation in tomato seedlings

Molecular hydrogen–induced salinity tolerance requires melatonin signalling inArabidopsis thaliana

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