Hydrogen Inhalation Ameliorates Oxidative Stress in Transplantation Induced Intestinal Graft Injury

Buchholz, Bettina M.; Kaczorowski, David J.; Sugimoto, Ryujiro; Yang, Runkuan; Wang, Y.; Billiar, Timothy R.; McCurry, Kenneth R.; Bauer, Anthony J.; Nakao, Atsunori

doi:10.1111/j.1600-6143.2008.02359.x

Cited by 283 publications

(233 citation statements)

References 44 publications

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“…Other studies reported that H 2 can downregulate the expressions of pro-inflammatory cytokines such as TNF-α, IL-6, IL-1β, CCL2, etc. 18) Our results suggest that HW would improve the immunopathogenesis of AD by way of modulating the production of Th 2 and pro-inflammatory cytokines, similar to our previous results. 15) Since IL-10 level is strongly correlated to IgE titer, 28) and AD is often involved in the infiltration IgE, the significant reduction of the IL-10 level could lead to inhibition of IgE.…”

Section: )supporting

confidence: 81%

“…12,17) While there are several methods to deliver H 2 into the body including inhalation of H 2 gas, 18) injection of hydrogensaturated saline, 19) and drinking hydrogen water, 20) we utilized HW produced by electrolysis wherein H 2 was highly dissolved in PW passing through the hydraulic system of the generator, thereby producing characteristics such as neutral pH, extremely low ORP, and high DH.…”

Section: Discussionmentioning

confidence: 99%

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Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Yoon

Sajo

Ignacio

et al. 2014

Biological & Pharmaceutical Bulletin

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Atopic dermatitis (AD) is a chronically relapsing, pruritic, eczematous skin disorder accompanying allergic inflammation. AD is triggered by oxidative stress and immune imbalance. In the present study, we investigated the effect of drinking hydrogen water (HW) on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in NC/Nga mice and found that HW ameliorated DNCB-induced AD-like clinical symptoms. In line with this, the level of reactive oxygen species in the HW group was significantly inhibited compared with that in the purified water (PW) group. In parallel, HW enhanced glutathione peroxidase activity in DNCBinduced AD as compared with the PW group. Accordingly, the levels of thymus and activation-regulated chemokine and cytokines were significantly decreased in the HW group compared with the PW group. Notably, the levels of Th 2 cytokine, interleukin-5 (IL-5), and proinflammatory cytokines such as tumor necrosis factor-α and IL-6 in HW-fed mice were significantly lower than in control and PW-fed mice. The total serum immunoglobulin E level was also markedly reduced in the HW group. The collective results indicate that HW suppresses DNCB-induced AD in NC/Nga mice via redox balance and immune modulation and could be a safe clinical fluid treatment for AD.Key words hydrogen water; atopic dermatitis; oxidative stress; immune modulation Atopic dermatitis (AD) is a chronically relapsing, pruritic, eczematous skin disorder accompanying allergic inflammation.1,2) AD is characterized by an impairment of the skinbarrier function, increased oxidative stress, and dysfunctional immune system. Intense infiltration of inflammatory cells release bioactive substance such as cytokines, chemokines, and reactive oxygen species (ROS).3) Of these AD's etiologic factors, inflammatory cytokines are known to regulate skin barrier, thereby aggravating the eczematous reaction in AD. 4)Most acute skin lesions in AD are exhibited by Th 2 inflammatory cytokines, whereas the chronic phase is characterized by Th 1 immune responses.5) The imbalance of cytokine network would play a critical role in the development of AD. 6,7)Together, oxidative stress such as increased ROS and lipid peroxidation is evident in any stage of AD. [8][9][10] An imbalance between ROS and antioxidants can lead to an elevated oxidative stress level. 11)Hydrogen molecule (H 2 ) has been widely used in medical applications as a safe and effective antioxidant and immunomodulator with minimal side effects.12-15) Unlike other antioxidants, which are unable to target organelles, H 2 can penetrate biomembranes and diffuse into the cytosol, mitochondria and nucleus. 13) Moreover, H 2 has been reported to have •OHscavenging activity in culture medium.14) Oral administration of hydrogen water (HW), inhalation of hydrogen gas, and injecting H 2 -dissolved saline is widely accepted to incorporate H 2 in the body. Recently, we reported that HW is effective in house mite-induced dermatitis via immunomodulation. 15) However, this study has a limitation owing to the lack of th...

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Section: )supporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Yoon

Sajo

Ignacio

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Recently, the feasibility of the application of hydrogen to the treatment of diseases attracts attention in the medical field [1][2][3][4][5]. In an earlier work [6] on the application of hydrogen storage alloy (HSA) in the medical field, it has been found that hydrogen in a form of atomic hydrogen (hydrogen radical) causes the death of cancer cells, while no damage is detected in normal cells.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Cancer Cell Death Induced by Hydrogen Discharged from Palladium Base Hydrogen Storage Alloy

Matsuzaki

Kagawa

Motoishi

et al. 2013

AMR

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The mechanism of cancer cell death induced by hydrogen discharged from Pd-5at.% Ni hydrogen storage alloy has been investigated. Cancer cell (HeLa : cervical cancer cell) death was observed in the limited region within ~ 3 mm from the sample. The measurement of surviving fraction of cells revealed that almost all the cancer cells in the well of 96-well multi plate, 6.2 mm in diameter were extinct (p < 0.01), while no detectable influence was observed in the normal cells. From the fluorescent imaging experiment, it was indicated that the cell death induced by discharged hydrogen was due to the "Apoptosis" and hydrogen peroxide was detected in both intracellular and extracellular regions. Furthermore, the generation of hydrogen radical and hydroxyl radical was observed in the ESR measurement. From the results obtained, the mechanism of cancer cell death is proposed.

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“…Ohsawa et al (2007) reported that inhalation of hydrogen (H 2 ) gas could selectively mitigate ·OH damage in a rat model of middle cerebral artery occlusion, generating an antioxidant effect without affecting the signaling of other ROS. Further research demonstrated that the inhalation of H 2 gas produced protective effects on I/R injury in various settings, such as myocardial infarction (Hayashida et al, 2008), intestinal injury (Buchholz et al, 2008), and hepatic injury (Fukuda et al, 2007). Furthermore, H 2 has been reported to downregulate ConA-induced mouse liver inflammation (Kajiya et al, 2009) and to protect mice against multiple organ damage in a zymosaninduced generalized model of inflammation (Xie et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of hydrogen-rich saline on ischemia/reperfusion injury in rat skin flap

Zhao

Wang

Qin

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs in plastic surgery. The mechanisms of I/R injury include hypoxia, inflammation, and oxidative damage. Hydrogen gas has been reported to alleviate cerebral I/R injury by acting as a free radical scavenger. Here, we assessed the protective effect of hydrogen-rich saline (HRS) on skin flap I/R injury. Methods: Abdominal skin flaps of rats were elevated and ischemia was induced for 3 h; subsequently, HRS or physiological saline was administered intraperitoneally 10 min before reperfusion. On postoperative Day 5, flap survival, blood perfusion, the accumulation of reactive oxygen species (ROS), and levels of cytokines were evaluated. Histological examinations were performed to assess inflammatory cell infiltration. Results: Skin flap survival and blood flow perfusion were improved by HRS relative to the controls. The production of malondialdehyde (MDA), an indicator of lipid peroxidation, was markedly reduced. A multiplex cytokine assay revealed that HRS reduced the elevation in the levels of inflammatory cytokines, chemokines and growth factors, with the exception of RANTES (regulated on activation, normal T-cell expressed and secreted) growth factor. HRS treatment also reduced inflammatory cell infiltration induced by I/R injury. Conclusions: Our findings suggest that HRS mitigates I/R injury by decreasing inflammation and, therefore, has the potential for application as a therapy for improving skin flap survival.

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Hydrogen Inhalation Ameliorates Oxidative Stress in Transplantation Induced Intestinal Graft Injury

Cited by 283 publications

References 44 publications

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Mechanism of Cancer Cell Death Induced by Hydrogen Discharged from Palladium Base Hydrogen Storage Alloy

Protective effect of hydrogen-rich saline on ischemia/reperfusion injury in rat skin flap

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