Hydrogen Inhalation Reduces Lung Inflammation and Blood Pressure in the Experimental Model of Pulmonary Hypertension in Rats

Abstract

Hydrogen has been shown to exhibit selective antioxidant properties against hydroxyl radicals, and exerts antioxidant and anti-inflammatory effects. The monocrotaline-induced model of pulmonary hypertension is suitable for studying substances with antioxidant activity because oxidative stress is induced by monocrotaline. On day 1, male Wistar rats were subcutaneously injected with a water–alcohol solution of monocrotaline or a control with an only water–alcohol solution. One group of monocrotaline-injected animals was placed in a plastic box that was constantly ventilated with atmospheric air containing 4% of molecular hydrogen, and the two groups of rats, injected with monocrotaline or vehicle, were placed in boxes ventilated with atmospheric air. After 21 days, hemodynamic parameters were measured under urethane narcosis. The results showed that, although hydrogen inhalation had no effect on the main markers of pulmonary hypertension induced by monocrotaline injection, there was a reduction in systemic blood pressure due to its systolic component, and a decrease in TGF-β expression, as well as a reduction in tryptase-containing mast cells.

Characteristics of hydrogen rich water at different stages of electrolysis

“…The material for the study was four types of drinking water: "Holy Spring" (1), "Chernogolovka" (2), " Kubai " (3), "Lipetsk pump room" (4), as well as distilled (5) and tap water (6). In the first experiment, three samples of each type underwent electrolysis in the Lourdes apparatus HS -81" for 70 minutes.…”

“…Such biologically active additives also include natural inhibitors of free radical oxidation -antioxidants. Many authors consider the use of molecular hydrogen as an effective antioxidant to be one of the promising areas [2][3][4][5][6]. Molecular hydrogen is produced by electrolysis of water.…”