Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings

Li, Shi-Weng; Xue, Lingui; Xu, Shou‐Jun; Feng, Hao; An, Lizhe

doi:10.1016/j.envexpbot.2008.06.004

Cited by 121 publications

(92 citation statements)

References 47 publications

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“…Whilst transient increase of H 2 O 2 seems to be necessary for initiation of in vitro morphogenesis (Siminis et al 1994;Kairong et al 1999;Agrawal and Purohit 2012; data presented here), the long-lasting overproduction of H 2 O 2 was shown to cause severe oxidative stress which inhibits root formation (Li et al 2009a;Pal Singh et al 2009). In our study, the non-rhizogenic hypocotyls were characterized by low H 2 O 2 content at day 3 of culture and relatively high steady-state level at day 7 and 14.…”

Section: Prooxidants Strongly Affect Adventitious Roots Formation Andmentioning

confidence: 61%

“…Scale bar 1 mm the course of rhizogenesis in hypocotyls of M. crystallinum. Previously it was shown that non-enzymatic antioxidants such as GSH and AA are engaged in a sophisticated interactions between auxin and various components of cellular redox system during root formation (Tyburski and Tretyn 2010a;Li et al 2009a) reported that exposure of mung bean seedlings to AA decreased or blocked root differentiation depending on the concentration used. In our study, the addition of AA to auxin-containing medium resulted in total inhibition of rhizogenesis (Table 1; Fig.…”

Section: Gsh and Aa Affect Rhizogenesis In Opposite Mannermentioning

confidence: 99%

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Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Libik-Konieczny

Kozieradzka-Kiszkurno

Michalec-Warzecha

et al. 2017

Acta Physiol Plant

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The enrichment with antioxidants (glutathione or ascorbate) or prooxidants (alloxan, methylviologen, hydrogen peroxide) of root inducing medium significantly decreased rhizogenesis frequency (alloxan, hydrogen peroxide) or inhibited roots regeneration (ascorbate, methylviologen) during the in vitro culture of Mesembryanthemum crystallinum L. hypocotyls. The adventitious roots morphology, root hairs length and density, was also influenced. Changes in the rhizogenesis course were related to the differences in hydrogen peroxide concentration during following days of culture between explants exhibiting morphogenic potential and those without the ability to form adventitious roots. In explants with morphogenic potential, rhizogenesis induction was always accompanied by a high level of hydrogen peroxide followed by the decrease in H 2 O 2 content in following days. In contrast, in the explants without regeneration potential, the level of hydrogen peroxide was increasing during the culture period. Activity patterns of superoxide dismutase (SOD) and guaiacol peroxidase (POX) in the following days of culture were similar in the explants exhibiting regeneration potential cultured on different media. Total activity of SOD decreased during initial days of culture and then increased due to the activation of additional SOD isoform described as MnSODII. The activity of POX was low during the rhizogenesis induction, and then increased during following days of culture; the increase was correlated with the decrease in hydrogen peroxide content. In the explants without the ability to regenerate roots, the total activity of SOD was low throughout the whole culture period, whereas the POX activity was significantly higher than in hypocotyls with regeneration potential. It might be concluded that the increase in hydrogen peroxide during initial stages of rhizogenesis and the induction of MnSODII are prerequisites for adventitious roots formation from hypocotyls of M. crystallinum, independently in the presence of anti-or prooxidant in the culture medium.

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Section: Prooxidants Strongly Affect Adventitious Roots Formation Andmentioning

confidence: 61%

Section: Gsh and Aa Affect Rhizogenesis In Opposite Mannermentioning

confidence: 99%

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Libik-Konieczny

Kozieradzka-Kiszkurno

Michalec-Warzecha

et al. 2017

Acta Physiol Plant

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“…The accumulation of H 2 O 2 during root formation and its beneficial effects, at low concentrations, has been pointed out in several studies (Dunand et al, 2007;Li et al, 2009). Improvements in rooting were described when this substance was associated with auxins (Sebastiani and Tognetti, 2004;Franklin and Dias, 2011).…”

Section: Introductionmentioning

confidence: 93%

Can hydrogen peroxide and quercetin improve production of Eucalyptus grandis x Eucalyptus urophylla?

Prado¹,

Dionizio²,

Vianello³

et al. 2014

Afr. J. Biotechnol.

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Vegetative propagation is considered the best choice for the rapid multiplication of plant species, however, rooting may still present difficulties. Substances, such as auxins, phenolic compounds and hydrogen peroxide, are recognized as able to improve this process. The aim of the present work was to determine if hydrogen peroxide in combination with quercetin or indole butyric acid, can modify some characteristics related to rooting and development in cuttings of Eucalyptus grandis x Eucalyptus urophylla. Cuttings were periodically evaluated at 30, 60 and 90 days according to the following criteria: height, diameter and survival percentage. After planting (90 days), a destructive evaluation was performed to determine rooting percentage, average size and number of roots. Polyamines content and polyamine oxidase activity, as biochemical markers of plant development, were determined. No statistically significant differences in height, diameter, survival and rooting percentage, root length and number of roots per cuttings were found. Treatments induced a decrease in putrescine levels and polyamine oxidase activity in roots. For absence of positive responses, the use of these substances as a treatment to improve cutting production is economically unviable.

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“…Moreover, it has been reported that the increase of H 2 O 2 in the cells of some drought-resistant plants might be more related to a structural role than to oxidative damage (Munné-Bosch et al, 2001;Jubany-Marí et al, 2009). In addition, a number of studies focused on physiological function of exogenous H 2 O 2 which enabled plants to tolerate many kinds of abiotic stresses (Li et al, 2009;Gao et al, 2010;Ishibashi et al, 2011;Zhang et al, 2011). ROS is considered to be a part of stress-induced signal transduction, and H 2 O 2 regulates numerous expressions of genes and complex signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

Ma¹,

Wang²,

Sun³

et al. 2015

JAS

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Drought stress is a major challenge in agriculture, causing severe loss of crops throughout the world. Methyl jasmonate (MeJA) regulates a variety of plant developmental process and responds to many biotic and abiotic stresses. To explore the physiological mechanisms of drought stress mitigated by exogenous MeJA, we subjected callus of wheat (Triticum aestivum L.) to drought induced medium after pretreatment with 10 ml sterile solution that contained the following singly or in combinations: MeJA (0, 0.25, and 2.5µM) and the lipoxygenase (LOX) inhibitor salicylhydroxamic acid (SHAM; 0 and 0.5 mM) for 24 hours. The endogenous jasmonic acid (JA) content, activity of LOX, hydrogen peroxide (H 2 O 2 ) content, antioxidant enzymes activities, levels of reactive oxygen species (ROS), content of malondialdehyde (MDA), and cell viability were measured. The results showed that exogenous MeJA induced the activity of LOX and resulted in rapid increase in endogenous JA levels in a concentration-dependent manner; JA declined gradually after reaching its maximum. The level of H 2 O 2 increased with increases in LOX activity and endogenous JA level, which are involved in the octadecanoid signaling pathway. However, SHAM inhibited the LOX activity, the endogenous JA level, and the accumulation of H 2 O 2 in wheat callus. In addition, the activities of several antioxidant enzymes increased after MeJA-pretreatment, but this effect was also inhibited by SHAM. The cumulative results suggest that H 2 O 2 was generated through the octadecanoid signaling pathway, and some antioxidant defense genes were provoked by the accumulation of H 2 O 2 . Therefore, we inferred that H 2 O 2 might act as a signaling molecule for the MeJA-induced antioxidant defense. Drought stress can be improved by MeJA-pretreatment, leading to decrease in endogenous MDA and ROS contents, which are normally induced by drought conditions. Both drought stress and exogenous MeJA-pretreatment increased in LOX activity, endogenous JA level, and antioxidant enzymes. Combinatorial treatments showed an apparent synergistic effect on the activities of these antioxidant enzymes and resulted in improved cell viability. These results confirmed the hypothesis that H 2 O 2 acts as a signaling molecule for the MeJA-induced antioxidant defense and could alleviate the negative effects of drought stress on wheat callus.

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Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings

Cited by 121 publications

References 47 publications

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Can hydrogen peroxide and quercetin improve production of Eucalyptus grandis x Eucalyptus urophylla?

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

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