Hydrogen peroxide biosensor based on direct electrochemistry of soybean peroxidase immobilized on single-walled carbon nanohorn modified electrode

Shi, Lihong; Liu, Xiaoqing; Niu, Wenxin; Li, Haijuan; Han, Shuang; Chen, Jiuan; Xu, Guobao

doi:10.1016/j.bios.2008.07.001

Cited by 69 publications

(22 citation statements)

References 53 publications

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“…This shows the excellent application of SWCNTs matrices towards the direct determination of H 2 O 2 . Moreover, the exceptional properties of SWCNTs have made them extremely suitable for enzyme immobilization and thus they are exclusively used for enzyme based biosensor development [27,28]. Interestingly, Wang and co-workers reported the direct electrochemistry of CAT at SWCNTs-Au electrode surface [29].…”

Section: Nanomaterials Matrices Used For Cat Immobilizationmentioning

confidence: 99%

A Review on Direct Electrochemistry of Catalase for Electrochemical Sensors

Prakash

Umasankar

Chen

2009

Sensors

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Catalase (CAT) is a heme enzyme with a Fe(III/II) prosthetic group at its redox centre. CAT is present in almost all aerobic living organisms, where it catalyzes the disproportionation of H2O2 into oxygen and water without forming free radicals. In order to study this catalytic mechanism in detail, the direct electrochemistry of CAT has been investigated at various modified electrode surfaces with and without nanomaterials. The results show that CAT immobilized on nanomaterial modified electrodes shows excellent catalytic activity, high sensitivity and the lowest detection limit for H2O2 determination. In the presence of nanomaterials, the direct electron transfer between the heme group of the enzyme and the electrode surface improved significantly. Moreover, the immobilized CAT is highly biocompatible and remains extremely stable within the nanomaterial matrices. This review discusses about the versatile approaches carried out in CAT immobilization for direct electrochemistry and electrochemical sensor development aimed as efficient H2O2 determination. The benefits of immobilizing CAT in nanomaterial matrices have also been highlighted.

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Section: Nanomaterials Matrices Used For Cat Immobilizationmentioning

confidence: 99%

A Review on Direct Electrochemistry of Catalase for Electrochemical Sensors

Prakash

Umasankar

Chen

2009

Sensors

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“…However, the poor dispersibility of carbon nanotubes has traditionally been a major technical barrier to their application. Some researchers have shown that RTILs can assist in the dispersion of carbon nanotubes and impart useful properties in their application in bioelectronics [13][14][15][16][17][18]. In this communication, we report a simple method to immobilize enzymes in a cellulosemultiwalled carbon nanotube (MWCNT) matrix reconstituted by [EMIM][CH 3 COO], and on the subsequent use of this matrix to modify electrodes with glucose oxidase (GOx) as a model enzyme.…”

Section: Introductionmentioning

confidence: 99%

Direct electron transfer of glucose oxidase immobilized in an ionic liquid reconstituted cellulose–carbon nanotube matrix

Zhao

Varcoe

et al. 2009

Bioelectrochemistry

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“…Amperometric biosensors based on horseradish peroxidase (HRP) have emerged as the most convenient tools for H 2 O 2 detection due to their simplicity, high sensitivity, and selectivity (Li et al, 2009; Shi et al, 2009; Yao et al, 2006). To improve the performance and long-term stability of the enzyme electrode, effective immobilization of HRP to the transducer surface is of great importance (Cao et al, 2008; Wang et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Single bead-based electrochemical biosensor

Liu

Schrlau

Bau

2009

Biosensors and Bioelectronics

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A simple, robust, single bead-based electrochemical biosensor was fabricated and characterized. The sensor's working electrode consists of an electrochemically-etched platinum wire, with a nominal diameter of 25 μm, hermetically heat-fusion sealed in a pulled glass capillary (micropipette). The sealing process does not require any epoxy or glue. A commercially available, densely functionalized agarose bead was mounted on the tip of the etched platinum wire. The use of a pre-functionalized bead eliminates the tedious and complicated surface functionalization process that is often the bottleneck in the development of electrochemical biosensors. We report on the use of a biotin agarose bead-based, micropipette, electrochemical (Bio-BMP) biosensor to monitor H 2 O 2 concentration and the use of a streptavidin bead-based, micropipette, electrochemical (SA-BMP) biosensor to detect DNA amplicons. The Bio-BMP biosensor's response increased linearly as the H 2 O 2 concentration increased in the range from 1×10 −6 to 1.2×10 −4 M with a detection limit of 5×10 −7 M. The SA-BMP was able to detect the amplicons of 1 pg DNA template of B. Cereus bacteria, thus providing better detection sensitivity than conventional gel-based electropherograms.

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Hydrogen peroxide biosensor based on direct electrochemistry of soybean peroxidase immobilized on single-walled carbon nanohorn modified electrode

Cited by 69 publications

References 53 publications

A Review on Direct Electrochemistry of Catalase for Electrochemical Sensors

A Review on Direct Electrochemistry of Catalase for Electrochemical Sensors

Direct electron transfer of glucose oxidase immobilized in an ionic liquid reconstituted cellulose–carbon nanotube matrix

Single bead-based electrochemical biosensor

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