Hydrogen Peroxide Content and pH of Expired Breath Condensate from Patients with Asthma and COPD

Murata, Kazuya; Kitaguchi, Yoshiaki; Horiuchi, Toshimichi; Kubo, Keishi; Honda, Takayuki

doi:10.3109/15412555.2013.830094

Cited by 42 publications

(31 citation statements)

References 26 publications

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“…Although a nonsignificant trend was found, H 2 O 2 levels did not seem to be affected by asthma control [107]. In fact, another recent study found no significant correlations between H 2 O 2 levels and scores on the Asthma Control Test or FeNO level in stable asthma patients [108]. These last two findings, together with the wide variability that exists in the values of H 2 O 2 concentrations in healthy individuals, may affect the clinical applicability of these observations.…”

Section: Exhaled Breath Condensatementioning

confidence: 99%

Usefulness of Noninvasive Methods for the Study of Bronchial Inflammation in the Control of Patients with Asthma

Muñoz

Bustamante²,

López-Campos³

et al. 2015

Int Arch Allergy Immunol

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Bronchial asthma is one of the most prevalent respiratory conditions. Although it is defined as an inflammatory disease, the current guidelines for both diagnosis and follow-up of patients are based only on clinical and lung function parameters. Current research is focused on finding markers that can accurately predict future risk, and on assessing the ability of these markers to guide medical treatment and thus improve prognosis. The use of noninvasive methods to study airway inflammation is gaining increasing support. The study of eosinophils in induced sputum has proved useful for the diagnosis of asthma; however, its clinical implementation is complex. Some studies have shown that the measurement of exhaled nitric oxide (FeNO) may also be useful to establish disease phenotypes and improve control. Others have found that the measurement of pH and certain markers of oxidative stress, cytokines and prostanoids in exhaled breath condensate (EBC) may also be useful as well as the measurement of the temperature of exhaled breath and the analysis of volatile organic compounds (VOCs). In conclusion, since asthma is an inflammatory disease, it seems appropriate to try to control it through the study of airway inflammation using noninvasive methods. In this regard, the analysis of induced sputum cells has proved very useful, although the clinical implementation of this technique seems difficult. Other techniques such as temperature measurement, the analysis of FeNO, the analysis of the VOCs in exhaled breath, or the study of certain biomarkers in EBC require further study in order to determine their clinical applicability.

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Section: Exhaled Breath Condensatementioning

confidence: 99%

Usefulness of Noninvasive Methods for the Study of Bronchial Inflammation in the Control of Patients with Asthma

Muñoz

Bustamante²,

López-Campos³

et al. 2015

Int Arch Allergy Immunol

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“…Non-invasive monitoring of this physiological redox status using easily collected clinical samples such as exhaled breath condensate represents a promising approach for monitoring respiratory diseases such as asthma [2] and chronic obstructive pulmonary disease [3]. In the inflamed lung airways, activated eosinophils, macrophages, and neutrophils can produce superoxide (O 2 − ), which rapidly dismutates to form the relatively stable reactive oxygen species, H 2 O 2 [4,5].…”

Section: Introductionmentioning

confidence: 99%

A chemiluminescent platform for smartphone monitoring of H 2 O 2 in human exhaled breath condensates

Quimbar

Krenek

Lippert

2016

Methods

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Noninvasive measurement of oxidative markers in clinical samples has the potential to rapidly provide information for disease management, but is limited by the need for expensive analytical instrumentation that precludes home monitoring or point-of-care applications. We have developed a simple to use diagnostic platform for airway hydrogen peroxide (H2O2) that combines optimized reaction-based chemiluminescent designs with an inexpensive home-built darkbox and readily available smartphone cameras. Specialized photography software applications and analysis of pixel intensity enables quantification of sample concentrations. Using this platform, sample H2O2 concentrations as low as 264 nM can be detected. The platform has been used to measure H2O2 in the exhaled breath condensates of human subjects, showing good agreement with the standard Amplex Red assay.

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“…The following results suggest that EBC acidification may serve as a biomarker of the disease (asthma group vs. [19,27,29] or pediatric population [26,30,32]. The acidity of the sample is normalized when the patient's exacerbation period is properly treated [42].…”

Section: Asthmamentioning

confidence: 86%

A Review of the Usefulness of Non-invasive Exhaled Breath Condensate pH Analysis for Diseases Diagnosis with Elements of Meta-analysis: An Update from 2012

Muza¹,

Konieczna²,

Baczek³

2019

Pharm Anal Acta

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Exhaled breath condensate (EBC) sample analysis is an entirely non-invasive novel sample collection method that is fast, easy to perform, and effort-appeared independent. EBC samples can be very useful in identifying the biomarkers of many diseases. This review provides an updated overview of EBC pH disturbances in different disorders as well as physiological levels among healthy individuals since 2012. Our meta-analysis addresses some of the key questions related to sample processing before pH measurement and discusses various methods of condensate standardization that can be employed prior to conducting a pH assay. Given the recent widespread interest in research into the use of EBC to identify biomarkers, it is necessary to establish a pathway leading from analytical methods for biomarker evaluation using EBC pH to clinical applications of this technology. This review fills a gap in the literature and attempts to connect theory to practical analytical approaches to analyzing EBC samples and making critical treatmentrelated decisions next to the patient's bed.

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Hydrogen Peroxide Content and pH of Expired Breath Condensate from Patients with Asthma and COPD

Cited by 42 publications

References 26 publications

Usefulness of Noninvasive Methods for the Study of Bronchial Inflammation in the Control of Patients with Asthma

Usefulness of Noninvasive Methods for the Study of Bronchial Inflammation in the Control of Patients with Asthma

A chemiluminescent platform for smartphone monitoring of H 2 O 2 in human exhaled breath condensates

A Review of the Usefulness of Non-invasive Exhaled Breath Condensate pH Analysis for Diseases Diagnosis with Elements of Meta-analysis: An Update from 2012

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