Hydrogen Peroxide-induced Cell Death in Arabidopsis: Transcriptional and Mutant Analysis Reveals a Role of an Oxoglutarate-dependent Dioxygenase Gene in the Cell Death Process

Gechev, Tsanko; Minkov, Ivan; Hille, Jacques

doi:10.1080/15216540500090793

Cited by 98 publications

(71 citation statements)

References 33 publications

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“…A similar effect was observed by treating the roGFP2-seedlings with the catalase inhibitor 3-amino-1,2,4-triazole (3-AT; Fig. 1C; Gechev et al, 2005) or with exogenous H 2 O 2 (data not shown). In spite of the clear effect, it is difficult to assess whether the oxidation of the roGFP2 probe upon treatments with cadmium, 3-AT, or H 2 O 2 might be mediated by a partial oxidation of the glutathione pool or alternatively achieved by other means (e.g.…”

Section: Cadmium Induces Accumulation Of No and Cytosolic Oxidation Imentioning

confidence: 49%

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

et al. 2013

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NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous enzyme involved in the glycolytic pathway. It has been widely demonstrated that mammalian GAPDH, in addition to its role in glycolysis, fulfills alternative functions mainly linked to its susceptibility to oxidative posttranslational modifications. Here, we investigated the responses of Arabidopsis (Arabidopsis thaliana) cytosolic GAPDH isoenzymes GAPC1 and GAPC2 to cadmium-induced stress in seedlings roots. GAPC1 was more responsive to cadmium than GAPC2 at the transcriptional level. In vivo, cadmium treatments induced different concomitant effects, including (1) nitric oxide accumulation, (2) cytosolic oxidation (e.g. oxidation of the redox-sensitive Green fluorescent protein2 probe), (3) activation of the GAPC1 promoter, (4) GAPC1 protein accumulation in enzymatically inactive form, and (5) strong relocalization of GAPC1 to the nucleus. All these effects were detected in the same zone of the root tip. In vitro, GAPC1 was inactivated by either nitric oxide donors or hydrogen peroxide, but no inhibition was directly provided by cadmium. Interestingly, nuclear relocalization of GAPC1 under cadmium-induced oxidative stress was stimulated, rather than inhibited, by mutating into serine the catalytic cysteine of GAPC1 (C155S), excluding an essential role of GAPC1 nitrosylation in the mechanism of nuclear relocalization, as found in mammalian cells. Although the function of GAPC1 in the nucleus is unknown, our results suggest that glycolytic GAPC1, through its high sensitivity to the cellular redox state, may play a role in oxidative stress signaling or protection in plants.

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Section: Cadmium Induces Accumulation Of No and Cytosolic Oxidation Imentioning

confidence: 49%

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

et al. 2013

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“…WRKY75, together with the NPR1-dependent WRKY 46, are the only WRKYs detected here as early SAIGs, that have not been previously associated to pathogen-induced defense responses (Eulgem and Somssich 2007). WRKY75 has been reported to be involved in H 2 O 2 -induced cell death and as a modulator of phosphate acquisition and root development (Gechev et al 2005;Devaiah et al 2007). The function of ANAC102 and WRKY75 in the defense reaction in Arabidopsis remains to be characterized.…”

Section: Discussionmentioning

confidence: 96%

Early genomic responses to salicylic acid in Arabidopsis

et al. 2009

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Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early SAIGs); 193 by a NPR1-dependent and 24 by a NPR1-independent pathway. These two groups of genes also differed in their functional classification, expression profiles and over-representation of cis-elements, supporting differential pathways for their activation. Examination of the expression patterns for selected early SAIGs from both groups indicated that their activation by SA required TGA2/5/6 subclass of transcription factors. These genes were also activated by Pseudomonas syringae pv. tomato AvrRpm1, suggesting that they might play a role in defense against bacteria. This study gives a global idea of the early response to SA in Arabidopsis seedlings, expanding our knowledge about SA function in plant defense.

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“…There are also negative correlations to both antisense and insertional mutants of AOX1 linked to the production of O 2 .2 and H 2 O 2 , again in mitochondria (Umbach et al, 2005;Giraud et al, 2008), suggesting that mitochondria may play a role in ROS signaling during senescence in both tissues. Peroxisomal ROS also may act as a signal in petal senescence, as a good correlation was obtained to amidotriazole treatment (Gechev et al, 2005), thought to induce the production of H 2 O 2 in peroxisomes. Out of the 44 ROS-induced transcription factors analyzed by Rosenwasser et al (2011), approximately half (23) were up-regulated in one or more stages of dark-induced senescence in leaves.…”

Section: Ros Generation and Scavenging: Spatiotemporal Effectsmentioning

confidence: 98%

Production and Scavenging of Reactive Oxygen Species and Redox Signaling during Leaf and Flower Senescence: Similar But Different

2016

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ORCID IDs: 0000-0003-3830-5857 (H.R.); 0000-0001-6523-6848 (S.M.-B.).Reactive oxygen species (ROS) play a key role in the regulation of many developmental processes, including senescence, and in plant responses to biotic and abiotic stresses. Several mechanisms of ROS generation and scavenging are similar, but others differ between senescing leaves and petals, despite these organs sharing a common evolutionary origin. Photosynthesis-derived ROS, nutrient remobilization, and reversibility of senescence are necessarily distinct features of the progression of senescence in the two organs. Furthermore, recent studies have revealed specific redox signaling processes that act in concert with phytohormones and transcription factors to regulate senescence-associated genes in leaves and petals. Here, we review some of the recent advances in our understanding of the mechanisms underpinning the production and elimination of ROS in these two organs. We focus on unveiling common and differential aspects of redox signaling in leaf and petal senescence, with the aim of linking physiological, biochemical, and molecular processes. We conclude that the spatiotemporal impact of ROS in senescing tissues differs between leaves and flowers, mainly due to the specific functionalities of these organs.

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Hydrogen Peroxide-induced Cell Death in Arabidopsis: Transcriptional and Mutant Analysis Reveals a Role of an Oxoglutarate-dependent Dioxygenase Gene in the Cell Death Process

Cited by 98 publications

References 33 publications

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

Early genomic responses to salicylic acid in Arabidopsis

Production and Scavenging of Reactive Oxygen Species and Redox Signaling during Leaf and Flower Senescence: Similar But Different

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