Hydrogen peroxide-induced secondary necrosis in conidia of <i>Aspergillus fumigatus</i>

Oliveira, Manuela; Pereira, Clara; Bessa, Cláudia; Araújo, Ricardo

doi:10.1139/cjm-2015-0561

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…7A ). High concentration of H 2 O 2 can lead to excessive ROS accumulation and thus cell death ( 55 – 57 ), which might affect laccase production by fungal cells. We also found that an acceptable concentration of H 2 O 2 (5 mM) slightly reduced the abundance of GSH (by 4%), but significantly increased the abundance of GSSG (by 71%) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613

Zhang,

Qiu,

Qiu

et al. 2024

Microbiol Spectr

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The white rot fungus Cerrena unicolor 87613 has been previously shown to be a promising resource in laccase production, an enzyme with significant biotechnological applications. Conventional methods face technical challenges in improving laccase activity. Attempts are still being made to develop novel approaches for further enhancing laccase activity. This study aimed to understand the regulation of laccase activity in C. unicolor 87613 for a better exploration of the novel approach. Transcriptomic and metabolomic analyses were performed to identify key genes and metabolites involved in extracellular laccase activity. The findings indicated a strong correlation between the glutathione metabolism pathway and laccase activity. Subsequently, experimental verifications were conducted by manipulating the pathway using chemical approaches. The additive reduced glutathione (GSH) dose-dependently repressed laccase activity, while the GSH inhibitors (APR-246) and reactive oxygen species (ROS) inducer (H 2 O 2 ) enhanced laccase activity. Changes in GSH levels could determine the intracellular redox homeostasis in interaction with ROS and partially affect the expression level of laccase genes in C. unicolor 87613 in turn. In addition, GSH synthetase was found to mediate GSH abundance in a feedback loop. This study suggests that laccase activity is negatively influenced by GSH metabolism and provides a theoretical basis for a novel strategy to enhance laccase activity by reprogramming glutathione metabolism at a specific cultivation stage. IMPORTANCE The production of laccase activity is limited by various conventional approaches, such as heterologous expression, strain screening, and optimization of incubation conditions. There is an urgent need for a new strategy to meet industrial requirements more effectively. In this study, we conducted a comprehensive analysis of the transcriptome and metabolome of Cerrena unicolor 87613. For the first time, we discovered a negative role played by reduced glutathione (GSH) and its metabolic pathway in influencing extracellular laccase activity. Furthermore, we identified a feedback loop involving GSH, GSH synthetase gene, and GSH synthetase within this metabolic pathway. These deductions were confirmed through experimental investigations. These findings not only advanced our understanding of laccase activity regulation in its natural producer but also provide a theoretical foundation for a strategy to enhance laccase activity by reprogramming glutathione metabolism at a specific cultivation stage.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613

Zhang,

Qiu,

Qiu

et al. 2024

Microbiol Spectr

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“…Results of Angelova et al [53] indicate that exposure of fungal spores to H 2 O 2 promoted oxidative stress evidenced by inhibition of spore germination and biomass production. Oliveira et al [54] reported that H 2 O 2 induces conidia cell death in a dose-dependent manner and through a secondary necrosis mechanism.…”

Section: Resultsmentioning

confidence: 99%

Effects of Plant Extracts and Disinfectant Huva-San TR 50 on the Quality of Carrot (Daucus carota L.) Seeds

Gorski

Szopińska

Rosińska

et al. 2020

Ecological Chemistry and Engineering S

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The aim of the study was to determine the effect of Aloe vera (L.) Burm.f. (Aloe) and Morinda citrifolia L. (Indian mulberry) plant extracts and disinfectant Huva-San TR 50 (hydrogen peroxide stabilized with silver ions) on carrot seed quality. The seeds of two carrot cultivars Amsterdam (sample I) and Berlikumer 2 (sample II), were soaked in solutions of Aloe and Indian mulberry extracts and Huva-San TR 50 at concentrations of 0.025 %, 0.05 % and 0.1 % for 30 min. Untreated seeds and seeds soaked in distilled water for 30 min were used as controls. Seed germination was assessed after 7 and 14 days of incubation. The speed and uniformity of seed germination (vigour) were evaluated. Mycological analysis was performed using a deep-freeze blotter test. Sample I was characterized by lower germination at the first and the final counts than sample II and higher seed infestation with Alternaria radicina. Treating seeds of sample I with Morinda citrifolia plant extract at concentrations of 0.025 and 0.05 % reduced their infestation with Alternaria alternata and A. radicina, increased germination at the first and the final counts, and did not affect seed vigour. The effects of Aloe extract and disinfectant Huva-San TR 50 on seed quality parameters varied depending on their concentration. Hence, further studies are necessary to establish the optimal conditions for carrot seed treatment.

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Anti-Pythium activity of ZzAMP, a serine protease inhibitor (SPI) from Zingiber zerumbet rhizome, mediated by zoospore binding and oxidative stress

Raj

Nair²

2022

Eur J Plant Pathol

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Hydrogen peroxide-induced secondary necrosis in conidia of Aspergillus fumigatus

Cited by 5 publications

References 19 publications

Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613

Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613

Effects of Plant Extracts and Disinfectant Huva-San TR 50 on the Quality of Carrot (Daucus carota L.) Seeds

Anti-Pythium activity of ZzAMP, a serine protease inhibitor (SPI) from Zingiber zerumbet rhizome, mediated by zoospore binding and oxidative stress

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