Hydrogen Peroxide–Releasing Hydrogels for Enhanced Endothelial Cell Activities and Neovascularization

Lee, Yunki; Son, Joo Young; Kang, Jeon Il; Park, Kyung Min; Парк, Ки Донг

doi:10.1021/acsami.8b04522

Cited by 42 publications

(28 citation statements)

References 37 publications

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“…Low dosage H 2 O 2 could promote cell proliferation, while superfluous H 2 O 2 can lead to oxidative damage to cells. Therefore, after gelation, the releasing H 2 O 2 from HRP 1U ChOx 0.25U , HRP 1U ChOx 0.5U , and HRP 1U ChOx 1U hydrogels was quantified by Micro Hydrogen Peroxide (H 2 O 2 ) Assay Kit (Lee, Son, Kang, Park, & Park, 2018). From Figure 3a, higher ChOx activity resulted in more H 2 O 2 release, and the H 2 O 2 generation process could be sustained over 48 h and subsequently achieve a plateau.…”

Section: Resultsmentioning

confidence: 99%

Control the fate of human umbilical cord mesenchymal stem cells with dual‐enzymatically cross‐linked gelatin hydrogels for potential applications in nerve regeneration

Gao

et al. 2020

J Tissue Eng Regen Med

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Stem‐cell‐based therapy is a promising strategy to treat challenging neurological diseases, while its application is hindered primarily by the low viability and uncontrolled differentiation of stem cell. Hydrogel can be properly engineered to share similar characteristics with the target tissue, thus promoting cell viability and directing cell differentiation. In this study, we proposed a new dual‐enzymatically cross‐linked and injectable gelatin hydrogel for regulating survival, proliferation, and differentiation of human umbilical cord mesenchymal stem cells (hUC‐MSCs) in a three‐dimensional matrix. This injectable gelatin hydrogel was formed by oxidative coupling of gelatin–hydroxyphenyl acid conjugates catalyzed by hydrogen horseradish peroxidase (HRP) and choline oxidase (ChOx). Modulus and H2O2 release can be well controlled by ChOx activity. Results from calcein‐AM/PI staining and Ki67 immunofluorescence tests demonstrated that the survival and proliferation behavior of hUC‐MSCs were highly enhanced in HRP1UChOx0.25U hydrogel with lower modulus and less H2O2 release compared with other groups. Attractively, the expression of neuron‐specific markers β‐III tubulin, neurofilament light chain (NFL), and synapsin‐1 was significantly increased in HRP1UChOx0.25U hydrogel as well. Additionally, in vitro hemolysis test and in vivo HE staining data highlighted the good biocompatibility. Undoubtedly, this injectable gelatin hydrogel's ability to control hUC‐MSCs' fate holds enormous potentials in nervous disorders' therapy and nerve regeneration.

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Section: Resultsmentioning

confidence: 99%

Control the fate of human umbilical cord mesenchymal stem cells with dual‐enzymatically cross‐linked gelatin hydrogels for potential applications in nerve regeneration

Gao

et al. 2020

J Tissue Eng Regen Med

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“…Synthesis and Characterization of Gelatin−g−Hydroxyphenylpropionic Acid (GH) Hydrogels: GH polymer (phenolic content: 150 µm g −1 of polymer) was synthesized by using a EDC/NHS coupling reaction as previously described. [18,33] Briefly, the HPA solution (6 mmol) activated with EDC (6 mmol) and NHS (9 mmol) for 30 min was applied to the pre-heated gelatin solution (5 g dissolved in 150 mL of deionized water), and then reacted at 40 °C for 24 h. After the reaction, the resulting solution was dialyzed against distilled water, filtered, and lyophilized to obtain the GH polymer. The phenol conjugation and HPA content www.afm-journal.de www.advancedsciencenews.com…”

Section: Methodsmentioning

confidence: 99%

Engineered Heterochronic Parabiosis in 3D Microphysiological System for Identification of Muscle Rejuvenating Factors

Lee

Choi

Ahn

et al. 2020

Adv Funct Materials

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Exposure of a young systemic milieu reveals remarkable rejuvenation effects on aged tissues, including skeletal muscle. Although some candidate factors have been reported, the exact underlying mechanisms of putative rejuvenating factors remain elusive, mainly due to the experimental challenges of using in vivo parabiosis. An in vitro parabiosis system is reported here that integrates young-and old-muscle stem cell vascular niche on a 3D microfluidic platform designed to recapitulate key features of native muscle stem cell microenvironment. This 3D micro-physiological system enables mechanistic studies of cellular dynamics and molecular interactions within the muscle stem cell niche, especially in response to conditional extrinsic stimuli of local and systemic factors. Using engineered parabiosis system, it is demonstrated that vascular endothelial growth factor signaling from endothelial cells and myotubes synergistically contributes to the rejuvenation of the aged muscle stem cell function. Moreover, with the adjustable on-chip system, both blood transfusion and parabiosis can be mimicked and the time-varying effects of antigeronic and progeronic factors can be monitored in a single organ or multiorgan systems. The microfluidic system can be utilized as a complementary in vitro microphysiological model to aid the complex in vivo parabiosis studies.

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“…GH polymer (phenolic content: 150 µm/g of polymer) was synthesized by using a EDC/NHS coupling reaction as previously described (38,62). Briefly, the HPA solution (6 mmol) activated with EDC (6 mmol) and NHS (9 mmol) for 30 min was applied to the pre-heated gelatin solution (5 g dissolved in 150 mL of deionized water), and then reacted at 40 °C for 24 h. After the reaction, the resulting solution was dialyzed against distilled water, filtered, and lyophilized to obtain the GH polymer.…”

Section: Synthesis and Characterization Of Gelatin-g-hydroxyphenylpromentioning

confidence: 99%

Engineered Heterochronic Parabiosis in 3D Microphysiological System for Identification of Muscle Rejuvenating Factors

Lee

Choi

Ahn

et al. 2020

Preprint

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Exposure of aged mice to a young systemic milieu revealed remarkable rejuvenation effects on aged tissues, including skeletal muscle. Although some candidate factors have been identified, the exact identity and the underlying mechanisms of putative rejuvenating factors remain elusive, mainly due to the complexity of in vivo parabiosis. Here, we present an in vitro muscle parabiosis system that integrates young-and old-muscle stem cell vascular niche on a threedimensional microfluidic platform designed to recapitulate key features of native muscle stem cell microenvironment. This innovative system enables mechanistic studies of cellular dynamics and molecular interactions within the muscle stem cell niche, especially in response to conditional extrinsic stimuli of local and systemic factors. We demonstrate that vascular endothelial growth factor (VEGF) signaling from endothelial cells and myotubes synergistically contribute to the rejuvenation of the aged muscle stem cell function. Moreover, with the adjustable on-chip system, we can mimic both blood transfusion and parabiosis and detect the time-varying effects of anti-geronic and pro-geronic factors in a single organ or multi-organ systems. Our unique approach presents a complementary in vitro model to supplement in vivo parabiosis for identifying potential anti-geronic factors responsible for revitalizing aging organs.

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Hydrogen Peroxide–Releasing Hydrogels for Enhanced Endothelial Cell Activities and Neovascularization

Cited by 42 publications

References 37 publications

Control the fate of human umbilical cord mesenchymal stem cells with dual‐enzymatically cross‐linked gelatin hydrogels for potential applications in nerve regeneration

Control the fate of human umbilical cord mesenchymal stem cells with dual‐enzymatically cross‐linked gelatin hydrogels for potential applications in nerve regeneration

Engineered Heterochronic Parabiosis in 3D Microphysiological System for Identification of Muscle Rejuvenating Factors

Engineered Heterochronic Parabiosis in 3D Microphysiological System for Identification of Muscle Rejuvenating Factors

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